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Articles list with the same subject [Real-time PCR] of Virologica Sinica
Porcine circoviruses (PCV) include PCV1, PCV2, and the new-emerging PCV3. PCV2 is pathogenic to pigs, but the pathogenicity of PCV3 in pigs is debatable. Recently, there have been frequent reports of PCV2 and PCV3 co-infections in clinical samples. Thus, it would be practical to develop a duplex ..., Abstract
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Tobacco mosaic virus (TMV) causes significant yield loss in susceptible crops irrigated with contaminated water. However, detection of TMV in water is difficult owing to extremely low concentrations of the virus. Here, we developed a simple method for the detection and quantifi cation of TMV in ..., Abstract
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Endogenous beta retroviruses (enJSRV) are highly homologous with Jaagsiekte sheep retrovirus (exJSRV),this exogenous retrovirus is the aetiological agent of ovine pulmonary adenocarcinoma (OPA). The aim of this study was to clarify the function of enJSRV and the immunological mechanisms of its ..., Abstract
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Chicken embryo fibroblasts (CEFs) are among the most commonly used cells for the study of interactions between chicken hosts and H5N1 avian influenza virus (AIV). In this study, the expression of eleven housekeeping genes typically used for the normalization of quantitative real-time PCR (QPCR) ..., Abstract
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One set of primers and a TaqMan probe were designed based on the nucleotide sequence within ORF2 of Porcine circovirus type 2 (PCV2). A quantitative TaqMan real-time PCR was developed for detecting PCV2 with highly specificity, reproducibility and more sensitivity than conventional PCR. Standard ..., Abstract
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