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Articles list with the same subject [T cell] of Virologica Sinica
Dengue virus (DENV) is a mosquito-borne virus belonging to the Flaviviridae family. There are 4 serotypes of DENV that cause human disease through transmission by mosquito vectors. DENV infection results in a broad spectrum of clinical symptoms, ranging from mild fever to dengue hemorrhagic ..., Abstract
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IκB kinase ε (IKKε) is a non-canonical IκB kinase that is extensively studied in the context of innate immune response. Recently, significant progress has been made in understanding the role of IKKεin interferon (IFN) signaling. In addition to its roles in innate immunity, recent studies also ..., Abstract
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HEp-2 cells persistently infected with respiratory syncytial virus (RSV) are a heterogeneous mixture of viral antigen-positive and -negative variants; however, the mechanism through which viral replication becomes latent remains unclear. In this study, we investigated the potential mechanism by ..., Abstract
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HIV-1 envelope glycoprotein is reported to interact with α4β7, an integrin mediating the homing of lymphocytes to gut-associated lymphoid tissue, but the signifi cance of α4β7 in HIV-1 infection remains controversial. Here, using HIV-1 strain BaL, the ..., Abstract
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A novel avian influenza A (H7N9) virus recently emerged in the Yangtze River delta and caused diseases, often severe, in over 130 people. This H7N9 virus appeared to infect humans with greater ease than previous avian infl uenza virus subtypes such as H5N1 and H9N2. While there are other potential ..., Abstract
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The suppressive capacity of regulatory T cells (Tregs) has been extensively studied and is well established for many diseases. The expansion, accumulation, and activation of Tregs in viral infections are of major interest in order to find ways to alter Treg functions for therapeutic benefit. Tregs ..., Abstract
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Plasmid DNA, an effective vaccine vector, can induce both cellular and humoral immune responses. However, plasmid DNA raises issues concerning potential genomic integration after injection. This issue should be considered in preclinical studies. Tiantan vaccinia virus (TV) has been most widely ..., Abstract
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Lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several Bombyx cells. They have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cell lines. In this paper, we report the development of ht cell lines from other ..., Abstract
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To obtain the P8 protein of Rice gall dwarf virus (RGDV) with biological activity, its outer coat protein gene S8 was expressed in Spodoptera frugiperda (Sf9) insect cells using the baculovirus expression system. The S8 gene was subcloned into the pFastBacTM1 vector, to produce the recombinant ..., Abstract
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The occurrence of massive CD4+ T cell depletion is one of the most prominent characteristics of human immunodeficiency virus type 1 (HIV-1) infection during acute phase, resulting in unrestorable destruction to the immune system. The infected host undergoes an asymptomatic period ..., Abstract

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Herpes simplex viruses (HSV-1 and HSV-2) cause global morbidity and synergistically correlate with HIV infection. HSV exists life-long in a latent form in sensory neurons with intermittent reactivation, in despite of host immune surveillance. While abundant evidence for HSV interfering with innate ..., Abstract
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A recombination transposing vector pBlueBacHis2一CagA was constructed by inserting Heli—
cobacter pylori f口gA gene into pBlueBacHis2A vector of baculovirus expession system .Co—transfecting
Sf9 cells with Bac—N—blue DNA and pBlueBacHis2一CagA,recombinant viral DNA was obtained by ..., Abstract
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H antaan virus glycoprotein G1 and nucleoprotein partial fragment w ere connected in differ—
ent ways.The constructed chim eric gene G 1 SO .7 or SO.7G 1 was inserted into baculovirus expression
vector pFBD.The recombinant shuttle plasmids(Bacmids)containing chimeric genes were ..., Abstract
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Apoptosis of PK—t5 ceils induced by Foot—and-Mouth Disease Virus(FMDV)in vitro was
reported in this paper.Typical cell apoptosis was detected hy ti.se of Hoechst 33258 fluorescence probe.
agarose gel electrophoresis and in situ end-labeling(TUNEL).After PK一15 cells were infected by ..., Abstract
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The genes of C terminal of HCV NS3 region of two chronic patients in two different time were sequenced to analyse the conservation of a helper T cell epitope in HCV NS3 protein (amino acids 1248 to 1261). The epitope was synthesized and the immune response to it in a self limited patient and two ..., Abstract
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The genes of C terminal of HCV NS3 region of two chronic patients in two different time were sequenced to analyse the conservation of a helper T cell epitope in HCV NS3 protein (amino acids 1248 to 1261). The epitope was synthesized and the immune response to it in a self limited patient and two ..., Abstract
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The baculovirus insect cell expression system was used to prepare the HPV16 E 6 recombinant protein. The HPV16 E 6 complete ORF amplified by PCR from HPV16 genome was T A cloned into the baculovirus transfer vector pVL1393 T under AcMNPV Polh. This recombinant transfer plasmid pVL1393 E 6 DNA and ..., Abstract
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Distribution of silkworm Bm N cell attachment to Cytodex 3 microcarrier was observed and the influence of cell inoculation concentration and microcarrier concentration on cell growth was studied. The minimal cell inoculation concentration was 1.0×10 5 cells/mL when Cytodex 3 microcarrier ..., Abstract
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Full-length cDNA coding HCV core protein was isolated from human HCV positiveserum by RT-PCR and inserted into baculovirus transfer vector.Sf9 insect cells were co-transfect-ed with the recombinant transfer vector plasmid and wild type baculovirus DNA.The recombi-nant baculoviruses containing gene ..., Abstract
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The oil tree moth (B. suppressaria larvae) was grown to 4 instar using a seml-synthetic artificial diet. then infected with (BsNPV) inoculum propagated in the insect cell cultures. 7 days p.i., the virus-infected larvae were collected and virus (PIB) was purified by differential centrifugation. ..., Abstract
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