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Articles list with the same subject [fusion] of Virologica Sinica
Entry of severe fever with thrombocytopenia syndrome virus
Fei Yuan, Aihua Zheng
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a globe-shaped virus covered by a dense icosahedral array of glycoproteins Gn/Gc that mediate the attachment of the virus to host cells and the fusion of viral and cellular membranes. Several membrane factors are involved in virus entry ..., Abstract
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2017 Vol. 32 (1): 44
Characterization of two monoclonal antibodies, 38F10 and 44D11, against the major envelope fusion protein of Helicoverpa armigera nucleopolyhedrovirus
Zijiao Zou, Jinliang Liu, Zhiying Wang, Fei Deng, Hualin Wang, Zhihong Hu, Manli Wang, Tao Zhang
The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor (F0) and then cleaved into a disulfide-linked F1 and F2 subunits during the ..., Abstract
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2016 Vol. 31 (6): 490
Structure-function relationship of the mammarenavirus envelope glycoprotein
Wei Wang, Zheng Zhou, Leike Zhang, Shaobo Wang, Gengfu Xiao
Mammarenaviruses, including lethal pathogens such as Lassa virus and Junín virus, can cause severe hemorrhagic fever in humans. Entry is a key step for virus infection, which starts with binding of the envelope glycoprotein (GP) to receptors on target cells and subsequent fusion of the virus ..., Abstract
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2016 Vol. 31 (5): 380
Immunogenicity and protective efficacy of recombinant M2e. Hsp70c (Hsp70359-610) fusion protein against influenza virus infection in mice
Hamidreza Attaran, Hassan Nili, Majid Tebianian
New strategies in vaccine development are urgently needed to combat emerging influenza viruses and to reduce the risk of pandemic disease surfacing. Being conserved, the M2e protein, is a potential candidate for universal vaccine development against influenza A viruses. Mycobacterium tuberculosis ..., Abstract
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2014 Vol. 29 (4): 218-227
Surface Display of Rice Stripe Virus NSvc2 and Analysis of Its Membrane Fusion Activity*
ZHAO Shu-Ling, DAI Xue-Juan, LIANG Jian-Sheng, LIANG Chang-Yong
Rice stripe virus (RSV) infects rice and is transmitted in a propagative manner by the small brown planthopper. How RSV enters an insect cell to initiate the infection cycle is poorly understood. Sequence analysis revealed that the RSV NSvc2 protein was similar to the membrane glycoproteins of ..., Abstract
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2012 Vol. 27 (2): 100-108
Prokaryotic Expression and Potential Application of the Truncated PCV-2 Capsid Protein
LOU Zhong-Zi, LI Zhi-Yong, WANG Gang, LI Jian-Jiang, LAN Xi, LI Xue-Rui
Three pairs of specific primers were designed to amplify the F2-1, F2-2 and XF2-2 truncated sequences of ORF2 which encodes the capsid protein of porcine circovirus type 2 (PCV-2). The F2-1 sequence had most of the NLS region of ORF2, but the F2-2 and XF2-2 genes had the NLS region deleted. ..., Abstract
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2010 Vol. 25 (2): 86
Prevention and Treatment of KSHV-associated Diseases with Antiviral Drugs*
Ren-rong TIAN, Qing-jiao LIAO, Xulin CHEN
Kaposi’s sarcoma-associated herpesvirus (KSHV) was first identified as the etiologic agent of Kaposi’s sarcoma (KS) in 1994. KSHV infection is necessary, but not sufficient for the development of Kaposi sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman disease (MCD). ..., Abstract
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2008 Vol. 23 (6): 486-495
Expression and Characterization of the Fusion Protein of Newcastle Disease Virus Strain F48E8 in Insect Cells
WANG Gui-jun, QIN Ai-jian, SHAO Hong-xia, CUI Ping-fu, JIN Wen-jie
The fusion protein gene of strain F48E8 of Newcastle disease virus was amplified with RT-PCR, and cloned into the pGEM-T vector and named pGEM-NDF. Then, F gene was released from pGEM-NDF by digestion with BamHI and XbaI, and ligated to baculovirus transfer vector, pFastBac1, which was digested ..., Abstract
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2006 Vol. 21 (6): 571-574
Influence of Intramolecular Disulfide Bond on Fusion Core Structure of Envelope Protein of HERV
FENG Hui-xing, HUANG Li-qin, GONG Rui, PENG Xiao-xue, KANG Shu-li, TIEN Po, XIAO Geng-fu
Syncytin, a captive retroviral envelope protein, is possibly involved in the morphogenesis of the placental syncytiotrophoblast layer generated by trophoblast cell fusion. It is found that syncytin and HIV-1 envelope proteins share similar structural profiles and membrane fusion mechanisms. In this ..., Abstract
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2006 Vol. 21 (5): 437-442
JDV Tat has Strong Transactivate Ability Because of its RNA Binding Domain
DENG Gang, SU Yang, MU Jun-jie, LI Yue, QIAO Wen-tao, GENG Yun-qi, CHEN Qi-min
In order to find the cause of different transactivate abilities among JDV, BIV and HIV-1 Tat, four chimeric Tat cDNA expression constructs, JH, HJ, JB and BJ, were generated with the crossover points at the boundary of activation and binding domains based on functional domain division of HIV-1 Tat ..., Abstract
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2006 Vol. 21 (2): 142-147
Construction of a Recombinant HaSNPV Expressing GP64
WANG Shu, WANG Hua-lin, DENG Fei, WU Dong, LONG Gang, YUAN Li, HU Zhi-hong
There are two kinds of membrane fusion proteins encoded by baculovirus: GP64 and F protein. The membrane fusion protein of group I NPV is GP64, while group II NPV is F protein. In this paper. we tried to answer whether GP64 can be expressed and assembled into the BV of Group II NPV. By using ..., Abstract
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2005 Vol. 20 (1): 79-83
Preparation of Rabies Vaccine by Perfusion Culture of Vero Cells on Microcarriers
YUE Wei, YE Lin-bai, ZHANG Jie, LIU Jing
To establish a technique for preparing rabies vaccine by perfusion culture on microcarriers in bioreactor, a 5 litre bioreactor was filled with 199 cell culture medium which contains 10g/L of microcarriers. Then Vero cells were inoculated with a final cell density of 1×105/mL . After 7 days of ..., Abstract
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2004 Vol. 19 (4): 373-375
Construction of an Eukaryotic Expression Vector for SARS-CoV Sars7a and EGFP Fusion Protein Expression
HE Xian-hui, XU Li-hui, LIU Yi, CAI Xiao-chang, ZENG Yao-ying
Partial overlapping primers were designed based on the severe acute respiratory syndrome coronavirus (SARS-CoV) sars7a gene and were chemically synthesized. The sars7a gene fragment was obtained by two-round of PCR and this fragment was used as template for a further round of PCR by using a pair of ..., Abstract
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2004 Vol. 19 (3): 209-213
Expression of the Mature Protein Gene of Chicken γ-Interferon and the Detection for the Antiviral Activity of the Purified Expression Product
CAI Mei-hong, CAO Rui-bing, ZHOU Bin, TAN Guo-lei, YANG Song, CHEN Pu-yan
Using the total mRNA in the lymphocyte in chicken blood as the template, the mature protein gene of interferon γ-interferon was cloned and amplified through the reverse transcription polymerase chain reaction. The gene was then ligated to pRLC to establish the gene’s non-fusion expressing vector. ..., Abstract
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2004 Vol. 19 (1): 32-35
Construction and Expression of Eukaryotic Expression Vector Bearing Fusion Gene Of HBV PreS2+S Gene and IFN— Gene
CHEN Hongmei BAI Xue—fan, PAN Lei, LI Guang—yu, WEI San—hua, HUANG Chang—xing
To construct a recombinant eukaryotic expression vector bearing fusion gene Of Hepatitis B
viruS(HBV)S2+S gene and IFN-a gene，technique of splicing by overlapping extension and two
times PCR were used．Fusion gene fragment was obtained and directly cloned into pcDNA3．1 V5／His
TOPO TA ..., Abstract
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2002 Vol. 17 (4): 312-314
Fusion Expression of Hepatitis C Virus Core Region and NS3 Region in E ．coli
LI Wei-Dong, LIANG Bu-Feng, QI Zi-Bai
The gene encoding the core and NS3 proteins of hepatitis C virus was amplified by PCR, respectirely. Two genes were fused and formed the recombinant plamid pHCV CN (Core+NS3) and pHCV NC (NS3+Core). The fused plasmid were expressed in E.coli 06. The pHC CN plasmid expressed fussion protein was ..., Abstract
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2000 Vol. 15 (2): 204-207
Cloning and Expression in E ．coli of Nucleocapsid Protein Gene of Rice Grassy Stunt Virus
ZHANG Chun-Mei, TUN Jie-Jian, LIN Ai-Yang, XIE Lian-Hui
Based on the known RNA sequence of rice grassy stunt virus , IRRI isolate (RGSV IR), the cDNA of nucleocapsid protein (NCP) gene was obtained by RT PCR, with genomic RNAs of Shaxian isolate (RGSV SX) as template. The cDNA was then cloned into pGEM T vector and sequenced. The results showed that the ..., Abstract
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2000 Vol. 15 (2): 200-203
Solubilization Analysis of GST Fusion Protein Expressed in Baculovirus System
YANG Lin, LI Guo-Qing, HUANG Bao-Yang, WANG Quan-Zhong, LONG Qi-Xin, WANG Xun-Zhang
Insect Sf 9 cells were infected with constructed recombinant virus AcMNPV OCC - GST 6xHis Etp 28, which could produce GST fusion protein and the supernatant of 72 h pi cell lysate was examined by SDS PAGE. The results showed that GST 6xHis Etp 28 fusion protein of 53 kDa was expressed in insoluble ..., Abstract
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2000 Vol. 15 (2): 143-148
Identification and primary application of fusion-inhibiting active monoclonal antibody of respira- tory syncytial virus
HUANG Sheng-Hai, SHI Bai-Fen, WANG Meng-Li, ZHANG Yong-Na, HU Yong, BI Ke-Ju
Monoclonal antibody of F 3 hybridoma cell strain against RSV specific antigen was prepared by hybridoma and tested by indirect immunofluorescence and ELISA. It has neutralizing and fusion inhibiting activity. The neutralizing titres and fusion inhibiting titres of ascites of mice are 1∶128 and ..., Abstract
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2000 Vol. 15 (1): 34
Serological Relationships of Several Kinds of Peanut Viruses
LIU Wen-Bin, CHEN Kun-Rong, ZHANG Zong-Xi, HU Ze-Yong-
The serological relationships of several kinds of peanut viruses were analyzed by usingtechniques of agar diffusion test and ELISA. The results show that, among four main kinds ofviruses that infect peanut (PSV, PStV, TS and CMV), PSV, TS and PStV have strong infectingabilities, but CMV has poor ..., Abstract
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1999 Vol. 14 (4): 368-370
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