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Articles list with the same subject [replication] of Virologica Sinica
Dengue virus (DENV) belongs to the genus Flavivirus of the family Flaviviridae and it is primarily transmitted via Aedes aegypti and Aedes albopictus mosquitoes. The life cycle of DENV includes attachment, endocytosis, protein translation, RNA synthesis, assembly ..., Abstract
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Though efficient vaccines against hepatitis B virus (HBV) and antiviral therapies are available,chronic HBV infection is still a global health problem. The process of HBV infection and HBV life cycle are extensively studied in last decades, however, the mechanisms of HBV-induced alterations of host ..., Abstract
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Here, we introduce an additional method that we believe has merits with respect to reducing labor, decreasing wait times, improving objectivity, and decreasing long-term costs. This new method, called fluorescently labeled infected cell inoculum titration (FLICIT), utilizes high-throughput ..., Abstract
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Bovine foamy virus (BFV) is a complex retrovirus that infects cattle. Like all retroviruses, BFV encodes a transactivator Tas protein (BTas) that increases gene transcription from viral promoters. BFV encodes two promoters that can interact with BTas, a conserved promoter in the 5' long terminal ..., Abstract
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An amino acid mutation (R127→I) in the 3A non-structural protein of an FMDV serotype Asia1 rabbit-attenuated ZB strain was previously found after attenuation of the virus. To explore the effects of this mutation on viral replication and infection, the amino acid residue isoleucine (I) was changed ..., Abstract
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Dengue virus (DENV) nonstructural protein 1 (NS1) is a highly conserved 46-kDa protein that contains 2 glycosylation sites (Asn-130 and Asn-207) and 12 conserved cysteine (Cys) residues. Here, we performed site-directed mutagenesis to generate systematic mutants of viral strain TSV01. The results ..., Abstract
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Herpes simplex virus-1 (HSV-1) is a major pathogen that causes various central nervous system (CNS) diseases, including herpes simplex encephalitis and meningitis. According to recent studies, PNKP significantly affects the proliferation of HSV-1 in astrocytes. Here, we used viral proliferation ..., Abstract
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Flaviviral NS2B is a required cofactor for NS3 serine protease activity and plays an important role in promoting functional NS2B-NS3 protease configuration and maintaining critical interactions with protease catalysis substrates. The residues D80DDG in West Nile virus (WNV) NS2B are ..., Abstract
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Chilo iridescent virus (CIV) is the type species for genus Iridovirus, and belongs to the family Iridoviridae. Since the discovery of CIV in 1966, many attempts were made to elucidate the viral genome structure. The virions contain a single linear ds DNA molecule that is circularly permuted and ..., Abstract
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Baculoviruses were first identified as insect-specific pathogens, and it was this specificity that lead to their use as safe, target specific biological pesticides. For the past 30 years, AcMNPV has served as the subject of intense basic molecular research into the baculovirus infectious cycle ..., Abstract
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MicroRNAs (miRNAs) play important roles in eukaryotes, plants and some viruses. It is increasingly clear that miRNAs-encoded by viruses can affect the viral life cycle and host physiology. Viral miRNAs could repress the innate and adaptive host immunity, modulate cellular signaling pathways, and ..., Abstract
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Lentiviral vectors have drawn considerable attention recently and show great promise to become important delivery vehicles for future gene transfer manipulation. In the present study we have optimized a protocol for preparation of human immunodeficiency virus type-1 (HIV-1)-based defective ..., Abstract
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Baculoviruses are the only nuclear replicating DNA-containing viruses that encode their own DNA-directed RNA polymerase (RNAP). The baculovirus RNAP is specific for the transcription of genes expressed after virus DNA replication. It is composed of four subunits, making it the simplest multisubunit ..., Abstract
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he RdRp gene of Foot-and-mouth disease virus was amplified by reverse transcription polymerase chain reaction (RT-PCR) using pfuultraTM High-Fidelity DNA polymerase, and was then cloned into the prokaryotic expression vector pET-28a (+). E.coli BL-21 cells were transformed with the recombinant ..., Abstract
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Small interference RNA mixtures (siRNA Cocktails ) of Rabies virus (RV) N gene, P gene and G gene were prepared by digesting long double-stranded RNA (dsRNA) with RNase Ⅲ. These siRNA cocktails were transfecteing into BSR or MNA cell monolayers and the direct immunofluores- cence assay (FA) was ..., Abstract
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Entire ORF of Hepsin was isolated from adjacent live tissue of HCC with RT-PCR. Hepsin gene was inserted into expressing vector pCMV-tag-HS. The recombinant vector pCMV-tag-HS was co-transfected into liver tumor line Hep G2.2.1.5 with plasmid encoding HBx protein. The results indicated that ..., Abstract
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The effect of oligodeoxynuclcotldes(ODN)OI1 replication of CSFV(Shimen strain)WSIS investigated in
this papor.Ph~phorthimte 01igedeox.vnuel·eoddes(PS-ODN)targeted to the~gion of the 3’,5._nonceding
re,on(NCR)as well SIS PS-ODNtargetedto NS3 gone region㈨maIke叭inhibitedthereplication ..., Abstract
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The structure of nuclear matrix in Tn5B1 cell infected with AcMNPV was observed by means of sequential cell fractionation and whole mount technique of electron microscopy. Whereas early infection of AcMNPV resulted in no dramatic change in the nuclear matrix morphologically, polyhedra formed in the ..., Abstract
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The growth characteristics of Human herpesvirus 7 local strain YY5 were investigated in cord blood mononuclear cells (CBMCs) and CD4 + lymphoblastic cell line SUPT1. Virus growth was demonstrated by cytopathic effect (CPE), percentage of dead cells count, indirect immunofluorescence test with viral ..., Abstract
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