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PLOS Pathog: 浙大王晓伟教授团队揭示昆虫传播植物病毒新机制
at :2018/2/2 11:16:16    

近日,国际知名病原学期刊PLoS Pathogens在线发表了浙江大学农业与生物技术学院王晓伟教授课题组的最新成果。该研究发现双生病毒在烟粉虱中肠上皮细胞内,利用管状网络结构直接由早期内体被分泌至上皮细胞的基底面,完成跨细胞运输。原文题为“Intracellular trafficking of begomoviruses in the midgut cells of their insect vector”。浙江大学昆虫科学研究所王晓伟教授为本论文通讯作者,昆虫科学研究所博士研究生夏文强为第一作者,硕士生梁燕迟瑶,博士生李隆赵静,及浙大刘树生教授参与了该项研究。该研究得到国家自然科学基金重大项目和973计划项目的资助。

 

 

在植物病毒的扩散过程中,由于植物本身几乎不动,病毒很难直接从一株植物侵染下一株植物。目前已知的近千种植物病毒中,约三分之二主要依赖媒介昆虫进行传播;双生病毒占目前已记录的全部植物病毒的三分之一,主要通过媒介昆虫烟粉虱进行传播。烟粉虱以循环型方式传播双生病毒,传播过程中病毒需要经过烟粉虱的肠道、血淋巴以及唾液腺,最终随唾液一起被分泌至植物中,这对病毒的传播至关重要。

 

王晓伟教授课题组研究发现植物双生病毒在穿过其昆虫媒介烟粉虱的肠道的过程中,可以利用上皮细胞的胞吞胞吐以及囊泡结构进行跨细胞运输,从而越过肠道上皮细胞对病毒的阻碍。双生病毒进入烟粉虱肠腔后,首先在肠道上皮细胞的尖端面利用网格蛋白介导的细胞內吞进入细胞内,形成由磷脂双分子层包裹的內吞小泡。然后內吞小泡与早期内体融合,病毒也随之进入早期内体。研究表明双生病毒在烟粉虱体内的运输依赖于早期内体及其管状网络结构,但不依赖于晚期内体、溶酶体、循环内体、高尔基体、内质网等其他结构。说明双生病毒可能在进入烟粉虱上皮细胞后直接由早期内体被运输至细胞基底面质膜并由此被分泌至血淋巴中图一
 

图一 双生病毒运输模式图。 双生病毒从肠腔内起始,经过细胞内吞、早期内体和细胞外排最终被运输到昆虫血淋巴中。这个过程中可能有部分病毒会被运往晚期内体以及溶酶体内进行降解。

 

本研究首次探究了双生病毒在烟粉虱中肠细胞内的运输过程,对各类囊泡结构在双生病毒运输过程中的作用进行了研究,揭示了双生病毒与动物病原性病毒截然不同的细胞内运输过程。这一发现进一步阐明了烟粉虱传播双生病毒的机理,为阻断烟粉虱在田间传播双生病毒提供了新的防控思路。

 

文章链接:

http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006866

 

Abstract:Begomoviruses are exclusively transmitted by whiteflies in a persistent circulative manner and cause considerable economic losses to crop production worldwide. Previous studies have shown that begomoviruses accumulate in vesicle-like structures in whitefly midgut cells and that clathrin-mediated endocytosis is responsible for their internalization. However, the process by which begomoviruses are trafficked within whitefly midgut cells remains largely unknown. In this study, we investigated the roles of vesicle trafficking in the transport of Tomato yellow leaf curl virus (TYLCV), a begomovirus that has spread to over 50 countries and caused extensive damage to a range of important crops, within midgut cells of whitefly (Bemisia tabaci). By disrupting vesicle trafficking using RNA silencing and inhibitors, we demonstrated that the early steps of endosomal trafficking are important for the intracellular transport of TYLCV in the whitefly midgut. In addition, our data show that, unlike many animal viruses, TYCLV is trafficked within cells in a manner independent of recycling endosomes, late endosomes, lysosomes, the Golgi apparatus and the endoplasmic reticulum. Instead, our results suggest that TYLCV might be transported directly from early endosomes to the basal plasma membrane and released into the hemolymph. Silencing of the sorting nexin Snx12, which may be involved in membrane tubulation, resulted in fewer viral particles in hemolymph; this suggests that the tubular endosomal network may be involved in the transport of TYLCV. Our results also support a role for the endo-lysosomal system in viral degradation. We further showed that the functions of vector early endosomes and sorting nexin Snx12 are conserved in the transmission of several other begomoviruses. Overall, our data indicate the importance of early endosomes and the tubular endosomal network in begomovirus transmission.

 

浙江大学昆虫科学研究所王晓伟教授介绍:

 

王晓伟教授主要从事重大农业害虫烟粉虱及其所传病毒病防控的基础研究。近年来在入侵烟粉虱特异性传播植物病毒的分子机制等方面取得了重要进展,以通讯作者在PNAS, Autophagy, New Phytologist, PLoS Pathogens, Journal of Virology等期刊发表论文40余篇,目前担任Bulletin of Entomological Research, Journal of Insect Science等4个SCI期刊编委。
 

 
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