Citation: Changjiang Weng, Li Huang, Guangqiang Ye. Joint deletion of multifunctional MGF505-7R and H240R genes generates a safe and effective African swine fever virus attenuated live vaccine candidate .VIROLOGICA SINICA, 2024, 39(3) : 355-357.  http://dx.doi.org/10.1016/j.virs.2024.04.007

Joint deletion of multifunctional MGF505-7R and H240R genes generates a safe and effective African swine fever virus attenuated live vaccine candidate

  • Corresponding author: Changjiang Weng, wengchangjiang@caas.cn
  • Received Date: 11 December 2023
    Accepted Date: 11 April 2024
    Available online: 30 April 2024
  • Highlights1. MGF505-7R and H240R are virulence-related genes.2. ASFV-ΔMGF505-7R-ΔH240R is attenuated.3. The candidate vaccine is safe and protective.

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    1. Huang L, Liu H, Ye G, Liu X, Chen W, Wang Z, Zhao D, Zhang Z, Feng C, Hu L, Yu H, Zhou S, Zhang X, He X, Zheng J, Bu Z, Li J, Weng C. 2023. Deletion of African Swine Fever vVirus (ASFV) H240R gene attenuates the virulence of ASFV by enhancing NLRP3-mediated inflammatory responses. J. Virol., 97: e0122722.

    2. Li D, Yang W, Li L, Li P, Ma Z, Zhang J, Qi X, Ren J, Ru Y, Niu Q, Liu Z, Liu X, Zheng H. 2021a. African swine fever virus MGF-505-7R negatively regulates cGAS-STING-mediated signaling pathway. J. Immunol., 206: 1844-1857.

    3. Li D, Zhang J, Yang W, Li P, Ru Y, Kang W, Li L, Ran Y, Zheng H. 2021b. African swine fever virus protein MGF-505-7R promotes virulence and pathogenesis by inhibiting JAK1- and JAK2-mediated signaling. J. Biol. Chem., 297: 101190.

    4. Li J, Song J, Kang L, Huang L, Zhou S, Hu L, Zheng J, Li C, Zhang X, He X, Zhao D, Bu Z, Weng C. 2021. pMGF505-7R determines pathogenicity of African swine fever virus infection by inhibiting IL-1beta and type I IFN production. PLoS Pathog., 17: e1009733.

    5. Li J, Song J, Zhou S, Li S, Liu J, Li T, Zhang Z, Zhang X, He X, Chen W, Zheng J, Zhao D, Bu Z, Huang L, Weng C. 2023. Development of a new effective African swine fever virus vaccine candidate by deletion of the H240R and MGF505-7R genes results in protective immunity against the Eurasia strain. J. Virol.: e0070423.

    6. Ramirez-Medina E, Rai A, Espinoza N, Valladares A, Silva E, Velazquez-Salinas L, Borca MV, Gladue DP. 2023. Deletion of the H240R gene in African swine fever virus partially reduces virus virulence in swine. Viruses, 15.

    7. Yang K, Xue Y, Niu T, Li X, Cheng M, Bao M, Zou B, Shi C, Wang J, Yang W, Wang N, Jiang Y, Yang G, Zeng Y, Cao X, Wang C. 2022. African swine fever virus MGF505-7R protein interacted with IRF7and TBK1 to inhibit type I interferon production. Virus Res., 322: 198931.

    8. Zhao D, Sun E, Huang L, Ding L, Zhu Y, Zhang J, Shen D, Zhang X, Zhang Z, Ren T, Wang W, Li F, He X, Bu Z. 2023. Highly lethal genotype I and II recombinant African swine fever viruses detected in pigs. Nat. Commun., 14: 3096.

    9. Zhou P, Dai J, Zhang K, Wang T, Li LF, Luo Y, Sun Y, Qiu HJ, Li S. 2022. The H240R protein of African swine fever virus inhibits interleukin 1beta production by inhibiting NEMO expression and NLRP3 oligomerization. J. Virol., 96: e0095422.

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    Joint deletion of multifunctional MGF505-7R and H240R genes generates a safe and effective African swine fever virus attenuated live vaccine candidate

      Corresponding author: Changjiang Weng, wengchangjiang@caas.cn
    • a. Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China;
    • b. Heilongjiang Provincial Key Laboratory of Veterinary Immunology, Harbin 150069, China

    Abstract: Highlights1. MGF505-7R and H240R are virulence-related genes.2. ASFV-ΔMGF505-7R-ΔH240R is attenuated.3. The candidate vaccine is safe and protective.

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