Figure 8个  Table 1个
    • Virus Mean virus titera (log10 pfu/ml ± SD) at the indicated temperature (℃) Mean titer in micec
      32 35 36 37 38 39 40 Nasal wash (log10 total pfu ± SD) Lung (log10 pfu/g tissue ± SD)
      wtRSV 6.9 ± 0.2 6.9 ± 0.1 6.8 ± 0.1 7.1 ± 0.1 7.1 ± 0.1 6.8 ± 0.1 6.8 ± 0.1 3.1 ± 0.1 4.4 ± 0.2
      rRSV-Long/A2cp 6.1 ± 0.1 6.1 ± 0.1 6.0 ± 0.1 6.0 ± 0.1 5.9 ± 0.1 5.8 ± 0.1 5.7 ± 0.1 2.6 ± 0.1 3.1 ± 0.1
      rRSV-Long/A2cpts 6.0 ± 0.1 5.2 ± 0.1 4.7 ± 0.1 < 1.0b < 1.0 < 1.0 < 1.0 2.3 ± 0.1 2.0 ± 0.1**
      rRSV-Long/A2cptsΔSH 5.4 ± 0.1 4.3 ± 0.2 4.2 ± 0.1 < 1.0b < 1.0 < 1.0 < 1.0 2.1 ± 0.1 1.9 ± 0.1*
      an = 3 replicates (at each temperature).
      bShut-off temperature is defined as the restrictive temperature at which a 100-fold or greater reduction compared to the titer observed at the permissive temperature of 32 ℃ and the lowest shut-off temperatures for each virus are italic.
      cGroups of five mice were administered 1 × 106 pfu of the indicated virus intranasally under light anesthesia on day 0 and sacrificed on day 4. Virus titer was determined in the nasal washes (log10 total pfu) and lung tissues (log10 pfu/g tissue).
      *The significant difference of RSV replication titers between nasal wash and lung tissue. All the results were shown as the representive of three independent experiments. Data were shown as mean ± SD. *P < 0.05, **P < 0.01.

      Table 1.  Characterization of the temperature sensitivity and attenuation (att) phenotypes of recombinant RSVs (rRSVs) in vitro and in vivo.