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Citation: Chengfeng Lei,  Jian Yang,  Jia Hu,  Xiulian Sun. On the Calculation of TCID50 for Quantitation of Virus Infectivity [J].VIROLOGICA SINICA.  http://dx.doi.org/10.1007/s12250-020-00230-5

On the Calculation of TCID50 for Quantitation of Virus Infectivity

  • Corresponding author: Xiulian Sun, sunxl@wh.iov.cn
  • Received Date: 10 March 2020
    Accepted Date: 15 April 2020
    Published Date: 26 May 2020
  • The most important property of a virus is its infectivity. To measure infectivity, one can assay viral replication in cells to obtain a titer for a given virus stock. A titer is defined as a given number of infectious viral units per unit volume, and an infectious unit is the smallest amount of virus that produces recognizable effects [e.g., cytopathic effect (CPE), dot blot immunoreactivity]. The median tissue culture infectious dose (TCID50) is defined as the dilution of a virus required to infect 50% of a given cell culture.

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    1. Bliss CI (1938) The determination of the dose-the proportion responding curve from small numbers. Q J Pharma Pharmacol 11:192–216

    2. Burleson FG, Chambers TM, Wiedbrauk DL (1992) Virology: a laboratory manual. Academic Press, London, pp 244–246

    3. Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, Cambridge Hierholzer JC, Killington RA (1996) Virus isolation and quantitation.In: Mahy BWJ, Kangro HO (eds) Virology methods manual.Academic Press, London, pp 25–46

    4. Kärber G (1931) Beitrag zur kollektiven behandlung pharmakologiseher reihenversuche. Arch Exp Path Pharmacol 162:480–484

    5. LaBarre DD, Lowy RJ (2001) Improvements in methods for calculating virus titer estimates from TCID50 and plaque assays.J Virol Methods 96:107–126

    6. Lei CF, Sun XL (2018) Comparing lethal dose ratios using probit regression with arbitrary slopes. BMC Pharmacol Toxicol 19:61

    7. LeOra Software (2007) Polo-plus, POLO for windows. LeOra Software, Petaluma Meynell GG, Meynell E (1970) Theory and practice in experimental bacteriology, 2nd edn. Cambridge University Press, London, pp 205–255

    8. Reed LJ, Muench H (1938) A simple method of estimating fiftypercent endpoints. Am J Hyg 27:493–497

    9. Robertson JL, Jones MM, Olguin E, Alberts B (2017) Bioassays with arthropods, 3rd edn. CRC Press, Taylor & Francis Group, Boca Raton Spearman CI (1908) The method of ‘right and wrong cases’ (‘constant stimuli’) without Gauss’s formulae. Br J Psychol 2:227–242

    10. SPSS Inc. IBM Corp (2011) IBM SPSS statistics 20.0. SPSS Inc., Chicago Sun RY (1963) A practical combined method for computing the median lethal dose (LD50). Acta Pharm Sin 10:65–74

    11. Svensson L, Hjalmarsson A, Everitt E (1999) TCID50 determination by an immuno dot blot assay as exemplified in a study of storage conditions of infectious pancreatic necrosis virus. J Virol Methods 80:17–24

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    On the Calculation of TCID50 for Quantitation of Virus Infectivity

      Corresponding author: Xiulian Sun, sunxl@wh.iov.cn
    • 1 Wuhan Institute of Virology, Center for Biosafety MegaScience, Chinese Academy of Sciences, Wuhan 430071, China
    • 2 University of Chinese Academy of Sciences, Beijing 100049, China

    Abstract: The most important property of a virus is its infectivity. To measure infectivity, one can assay viral replication in cells to obtain a titer for a given virus stock. A titer is defined as a given number of infectious viral units per unit volume, and an infectious unit is the smallest amount of virus that produces recognizable effects [e.g., cytopathic effect (CPE), dot blot immunoreactivity]. The median tissue culture infectious dose (TCID50) is defined as the dilution of a virus required to infect 50% of a given cell culture.

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