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1986 Vol.01(2)

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Review

ON THE SDS-PAGE ANALYSIS OF 4 TYPES OF CELL CULTURE INFECTED WITH 4 STRAINS OF HERPES SIMPLEX VIRUS

ZHANG Tian-Ming, XIANG Jin-Min, FENG Jue-Sun

1986, 1(2): 1

Four types of cell culture,namely,HEP-2,HeLa,human embryo lung fibroblast and chicken embryo fibroblast were infected with 4 st- rains of herpes simplex virus,i.e.CC21,F,W,and G,the formal 2 of type 1 and W and G of type 2.The SDS-PAGE maps showed dif- ference between the maps form at 4h after infection and that at twenty four hours after virus infection,the electrophoresis maps showed a dif- ference between infected and uninfected cells.In the chicken embryo fi- broblast culture,there were difference betwe...

EFFECT OF SMALL DOSAGE OF INTERFERON ON VIRAL MARKERS OF CHRONIC HEPATITIS B

XIONG Kai-Jun, WANG Xin-He, JIN Wei-E, HUANG Hua-Fang, TAO Meng-Lin, ZENG Lian-Lan, HAN Cai-Yang, FAN Hong-Mei, WU Zhang-Qi, SUN Ji-Shan

1986, 1(2): 9

40 cases including chronic hepatitis B(30cases)and viral carriers(10 cases)were treated with interferon 32,ooo iu im,q.o.d,in a period of six months.The geometric average of HBsAg titer fall from 1:100.4 to 1:17.2 at the sixth months(P<0.001).21 of them(52、5%)became negat- ive.After six months 13 of 20 HBeAg positive cases turned into negative, anti-HBe appeared in 11 cases.With the four HBcAg positive cases,two of them became negative.DNAP were negative in 88.9% cases.73.1% pati- ents are negative for H...

THE STUDY OF DETERMINATION OF HBCAG IN PERIPHRAI BLOOD WITH RIA AND ELISA

HUANG Hua-Fang, JIN Wei-E, YAO Meng-Ling, XIONG Kai-Jun, WANG Xin-He, DAI Jia-Zhu, FAN Hong-Mei, ZENG Lian-Lan

1986, 1(2): 13

HBcAg is the constructional protein antigen of core component of HBV.It s presence in peripheral blood has been used as an important mar? ker of replication of HBV.We had studied the detection of HBcAg in 94 HBsAg positive cases of viral hepatitis with the methods of RIA and ELISA,and compared with the survey of HBeAg,anti-HBc and HBV- DNAp.HBcAg was found to be positive in sera of 28 cases(29.8%).The results suggested that the determination of HBcAg with the method of ELISA and RIA is comparatively sensiti...

PROPAGATION OF EHF VIRUS IN CELL CULTURES OF MERIONES UNGUICULATUS LUNG AND KIDNEY TISSUES

ZHU Zhi-Yong, TANG Han-Ying, WENG Jing-Qing, FU Gui-Ming

1986, 1(2): 19

3 strains of epidemic hemorrhagic fever(EHF)virus from lung tissues of Apodemus agrarius and Rattus norvegicus and blood of patient with EHF were inoculated in primary cell culture of Meriones unguiculatus lung and kidney tissues.In the first passage,a good multiplicaton was observed and TCID 50/ml of the primary cell culture of kidney tissues were 10~(6?0) (for R_3 strain)and 10~(6?5)(for B_5 strain).After passage 3,on the 2nd day after inoculation,specific immunofluorescence to EHF virus antigen was detec...

A STUDY ON THE NPV OF ARGROGRAMMA AGNATA STGR AND ANALYSIS OF ITS NUCLEAR ACID MAP WITH RESTRICTION ENDONUCLEASES

LUO Jing, WANG Han-Zhong, YANG Xue-Lou, WU Xi-Ying, HUANG Wen-Lin

1986, 1(2): 24

In this paper the morphology of NPV isolated from A.a.Stgr.is des- cribed and the map of A.a.NPV-DNA extracted in a simple method is obtained through restriction endonuclease digest such as EcoRI,BglⅠ, BglⅡ,BamHI,HindⅢ,and double digest BglⅠ+BglⅡ,BamHI+EcoRI.By using relationship between the different size of λ-DNA cleaved by EcoRI and it s mobility in 1% agarose electrophosis,standard curve of molec- ular weight is made.The molecular weight of each A.a.NPV-DNA fragments generated by restriction endonucleas...

ANALYSIS BY MONOCLONAL ANTIBODY OF TOBACCO MOSIAC VIRUS DETERMINANT AND ITS ANTIGEN TYPE

ZHANG Cheng-Liang, ZHANG Zuo-Fang, JUE Xiao-Feng, CHEN Jing, HU Wei-Zhen, SONG Shu-Min, LIU Jia-Jue, SUN Yue-Ying, CHEN Bo-Quan, TIAN Bo, JI Liang

1986, 1(2): 32

Fourteen isolates of Tobacco Mosiac Virus(TMV)were collected from 9 different regions of China.They were multiplied under isolating con- ditions and purified and measured by the same method.The concentra- tion series were started from 20μg/ml and then were diluted into nine dilutions from 10~(-1).10~(-2)…to 10~(-9)(contrasted with healthy host).Each was reacted individually with 10 TMV monoclonal antibodies of optimal dilution(selected through detecting titers)in ELISA(contrasted with poly- clonal antibody ...

SEROTYPE A STAPHYLOCOCCAL BACTERIOPHAGE CONVERTING STAPHYLOKINASE

WANG Jia-Xun, DAI Xiao-Mi, CI Ti-Dong, REN Jing

1986, 1(2): 38

Two serotype A temperate phages were isolated from staphylococcus aureus strain 120.One of them has the capacity of lysogenic conversion for production of staphylokinase,it has not been reported previously.

STUDIES ON THE NUCLEAR POLYHEDROSIS VIRUS DISEASE OF SUCRA JUJUBA CHU

SUN Fa-Ren

1986, 1(2): 42

STUDEIS ON THE ETIOLOGY OF CANKEROUS TUBER OF LIRIOPE SPICATA

WANG Ji-Tao, ZHAO Yi-Wen, WANG Zong-Kai

1986, 1(2): 46

ISOLATION AND IDENTIFICATION OF A VIRUSES INFECTING CYCLAMEN PLANTS

ZHOU Lv-Qian, Sun Fu-Lin, CHEN Li-Li, XIA Xiu-Qin, XIONG Ke-Juan, XIAN Zhi-Hui, FU Xin-Sheng

1986, 1(2): 50

BIOASSAY OF AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS AGAINST PECTINOPHORA GOSSYPIELLA LARVAE

ZHANG Zhong-Xin, ZHANG You-Qing, WANG Xiao-Rong, YUAN Yan-Xiu

1986, 1(2): 54

BRIGHT AND DARK FIELD ELECTRON MICROSCOPY OF NUCLEAR ACID MOLECULES OF SOME VIRUS

YUAN Li, WANG Xue-Lan, DENG Hai-Fan

1986, 1(2): 58