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1986 Vol.01(4)

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Review

THE FUTURE OF VIROLOGY

Fred Rapp, LIU Gang

1986, 1(4): 1

CLASSIFICATION AND ITS SIGNIFICANCE OF INSECT VIRUSES

XIE Tian-En

1986, 1(4): 3

ETIOLOGICAL AND EPIDEMOLOGICAL STUDIES OF EPIDEMIC HEMORRHAGIC FEVER(EHF) IN SICHUAN ENDEMIC AREA

CHEN Li-Li, XIE Yun-Ju, CHEN Shang-Zhi, ZHANG Shang-Gui, YAN Ci-You, ZHENG Guo-Ying, QIN Guang-Ming, XU Feng-Qin, TANG Wen-Ting, ZHANG Jian, WU Ying-Chao, LIU Cheng-Jin, ZHANG Chuan-An, TAO Guo-Fang

1986, 1(4): 18

It is the first time to isolate 10 strains of EHF viruses from Rattus norvegicus, Rattus flavipetus, A. Sguamipes, C. russula and EHF Patients in Sichuan endemic area. Serological identification of these strains was made by IFAT and ELISA techinques. Preliminary antigenicity analysis of the viruses was carried ou with monoclonal antibodies of EHFV. The resuhs showed that all the strains tested are antigenically closely related to A_9 and Hantaan virus 76—118 strains that belong to Apodemus type. It was not ...

AN EXPERIMENTAL STUDY ON VERTICAL TRANSMISSION OF EHFV

LIU Jiang-Qiu, LI Zhong-Yi, SHEN Bao-Jun, WEN Qing-Li, LIU Yu-Qiu, JIN Xian-Tao, HE Yi-Xiang

1986, 1(4): 25

This paper is a study on vertical transmission of EHFV. Balb/c mice were used as tne experimental animals of EHFV infection and IF technique was adopted The experiment result showed that the EHFV could be transmitted from the pregnant mice to their fetuses through the placenta. The EHFV could be found in many organs of the fetuses and isolated from them. At the same time, the specific IgG antibodies could be detected in the fetal blood, The EHFV isolated from the fetuses was confirmed by specific blocking t...

ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO

LI Cheng-Ming, CHEN Li-Li, YIN Da-Chang, CHEN Dan-Lin, LIU Li-Hua, WANG Hong-Xi, HU Ping

1986, 1(4): 30

A Strain of hepatitis A virus(S_(84-1))was directly isolated from a fecal specimen of acute patient with hepatitis A and serially passaged in human diploid fibroblast(2BS). Some conditions of cultivation of this virus were also studied. Following serial propagation, the incubation time of S_(84-1) virus was reduced from one and half months(passage 2) to 3 days(passage 12). S_(84-1) virus, without cytopathie effect, can t be detected in supernatant fluid by ELISA and was harvested by freezing-throwing the ce...

MORPHOLOGICAL COMPARISON OF HEMORRHAGIC FEVER WITH RENAL SYNDROME(HFRS) AND XINJIANG HEMORRHAGIC FEVER(XHF) VIRUSES

LI De-Rong, HE Jin-Fang, SONG Guang-Chang, LI Zhong-Duo, WANG Hong-Xia, LI Yu-Chuan, ZHU Guan-Fu

1986, 1(4): 37

Seven strains of HFRS virus were studied by means of thin-section electron microscopy. The XHF virus defined asa member of Bunyarividae was compared. The results showed that HFRS viruses including Hantaan virus strain 76-118 were different in morphology and maturity from the XHF virus. The HFRS viruses may be classified as a member of the new genus of Bunyavividae.

THE PRODUCTION OF AN ANTI-NSV-1 LYMPHOCYTE HYBRIDOMA CELL LINE AND TYPING OF HSV ISOLATES WITH MONOCLONAL AHTIBODIES

GAO Qian, JIANG Chao-Zhun, MA Wen-Yu, ZHONG Fang-Yang, CA Xiao-Ning, HONG Mei-Xian

1986, 1(4): 43

A hybridoma cell line(Mad-2) was produced by fusing mouse myloma cell line Sp2/O with the spleen cells immunized by HSV-1 SM44 strain infected BHK-21 cells and membrane protein antigen extracted from the HSV-1 infected ceils, the positive clone Mad-2 secreating monoclonal antibody(McAb) against HSV-1 was selected by ELISA. the supernatant and ascites producet by Mad-2 all reacted with HSV-1, hut not with HSV-2. the titer of McAb in ascites mesured by ELISA was 10~(-7) and the subclass of the Mad-2 was IgG1....

RESEARCHES ON THE HEMAGGLNTININ(HAN) OF HEMORRHAGIC FEVER WITH RENAL SYNDROME(HFRSV) Ⅱ. An Investigation of Factors Involving HA and HI test

XIA Dong-Xiang, WANG Mei-Xian, NI Da-Shi, JIANG Shao-Chun, MA Wen-Yu

1986, 1(4): 49

In this paper, the factors involving HA and HI test were reported It was observed that the preparation of HFRSV-HAN and the conditions for HA test were strict. The HAN was preserved well at 4℃ and seemed tolerabe to heat to some degree. The optimal pH ranged from 5.7 to 6.1, and the goose erythrocyte was most sensitive to the HAN among 4 sorts of red blood cells. The chloroform method for the removal of non-specific inhibitors from serum was proved both effective and convenient. It is concluded that with th...

OBSERVATIONS ON THE DISPERSION OF INCLUSIONS BODIES OF INSECT VIRUS BY BIRDS IN FOREST

DAI Guan-Qun, Chen Qiang-Xiong, XI Bing-Cai, QIAN Guo-Cai

1986, 1(4): 56

In this paper, 109 species of birds belonging to 31 families and 436 samples were detected, pH of all birds alimentary tracts were 5.0—7.0. The feces of birds remained infective in bioassay and PIB of virus could be detected in birds feces within 6 hr. after feeding. In natural forest, Number of birds with PIB was nearly 1%.

STUDIES ON A CYTOPLASMIC POLYHEROSIS VIRUS FOR CONTROLLING MASSON—PINE CATERPILLAR DENDROLIMUS PUNCTATUS WALKER

LIU Qing-Lang, WU Ruo-Guang, ZENG Chen-Xiang

1986, 1(4): 65

The Masson-pine caterpillar is the most serious pest of pinus spp. in southern China. we obtained a CPV of the masson-pine caterpillar (DCPV)in the Guang-zhou district in 1974. The polyhedra of DCPV are hexagonal in shape but vary in size from 0.5 to 6 μm in diameter. The virus particles are hexagonal in shape and range from 42 to 56 nm in diameter, we have been engaged in this research for ten years. The high infeciivity of DCPV has been shown: the Lc50 is 2.5 million polyhedra/ml for third to fourth insta...

MORPHOGENESIS OF PHiLOSAMIA CYNTHIA RICINI D.NPV IN THE CELL CULTURE

LIANG Bu-Feng, XIE Tian-En

1986, 1(4): 73

The assembly processes of the philosamia cynthia ricini D. NPV are observed by electron microscopy of sections of infected cell cultures. The synthesis and envelopment of the virus nucleoeapsid and stage in the formation of the polyhedral inclusion bodies are described, Studies of the Philosamia cynthia ricini D. NPV morphogenesis indicate that is typical events of baculovirus replications.

INFLUENCE OF DIFFERENT TEMPERATURES ON THE REPLICATION OF BUZURA SUPPRESSARIA NPV IN OVARY CELL LINE

WANG Lu-Ming, Zhang-Yang-Lian, XIE Tian-En

1986, 1(4): 80

This experiment is basad on the infection of Buzura Suppressaria ovary cell line by Buzura Suppressaria NPV. After static incubation of the infected cell line at different temperatures. 0℃, 15℃, 20℃, 26℃, 28℃,30℃, and 37℃, the cytopathologieal effect have been observed by the determination of thepercentage of the infected cells and the yield of polyhedra. The result has been shown that the infection rate of the virus during their replication in cells is obviously influence at different temperature. The opti...

THE PRIMARY INVESTIGATION OF EPIDEMIOLOGY ON THE BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS(NPV)

PENG Hui-Yin, ZENG Yun-Tian, XIE Tian-En

1986, 1(4): 87

Buzura suppressria NPV has been the most widely used virus by artificial dissemination of NPV in the tea plantation with trial conducted in Hubei, Guangdong. Jiangxi go on. In puqi, in 1979, 1981, 1983. dosages of 2×10~6PIB/ml, were tested and Tea garden were sprayed to 750 liter/ha. At 10 days postspray these dosages gave 97.8% mortality, with 12% mortality, respectively, with 15% mortality on untreated Any way, BsNPV had maintained in a viable state on foliage for more days or several years. The polyhedra...

SYNTHESIS OF CITRUS EXOCORTIS VIROID AND CHRYSANTHEMUM STUNT VIROID DNA ~(32)P-cDNA PROBES AND DETECTION BY THE PROBES

ZHANG Qi-Ya, LIU Yong, YANG Ping, LIN Mu-Lan, WENG Zhao-Kui, MI Ke-Yong, ZHUO Zhong-Qi, DING Da-Ming, YANG Xi-Cai, CHEN Wei, TIAN Bo

1986, 1(4): 93

The ~(32)P-cDNA Probes of Citrus Exocortis Viroid (CEV) and Chrysanthemum Stunt Viroid (CSV)RNA has been synthesied respectively and usedfor detecting some samples that may contain relative viroids. This is a sensitive and rapid method for diagnosing early viroid disease as compared with biology assays and polyacrylamide gel electrophoresis,

A METHOD IN RECOVERY OF DNA FROM GELS

LIU Hai-Lin, Yang Ji-Gong, ZHENG Yi, SHI Jun, XIANG Jin-Min

1986, 1(4): 99