1987 Vol.02(1)
Comparision of influenza A (H_3N_2) viruses isolated from pig and human in 19791932 in respect to antigenic analysis, SDS-polyacrylamide gel electrophoreses and haemagglutinin maps has been studied. The isolates from pigs each year were very closely related to contemporary human isolates. The surface antigens, HA and NA, as well as the matrix proteins (MP) and nucleoproteins (NP) were very similar, The present findings provided some evidences for the very frequent transmission of influenza (H_3N_2) from hu...
Mouse lymphocyte hybridomas were prepared by polyethylene glycolmediated fusion of ceils from a mouse plasmaeytoma line (X63-Ag8-653) with lymphoeytes from Balb/c mice hyperimmunized with dengue virus type Ⅰ (Hawaii). Seven mouse lymphocyte hybridoma clones(C4, F5, F1, A2, B4, H7,D6) which can steadily produce antibodies against dengue-1 antigen were established after selecting and cloning for at least 3 times and have been kept in the laboratory for more than 5 months. Cross-immunofluorescent staining test...
This study showed that the treatment of pppA2 P5 A2 P5 A(2 -5 P_3A_3) on Lpa cells of mice in vitro makcs them possible to resist the challenge of Newcastle disease virus and vesicular stomatitis virus, giving further evidence for the general antiviral effect of 2′-5′P_3A_3.Conditions for getting positive antiviral effect of 2′-5′ P_3 A_3 were the quantity of challenging virus and the time for observing the results. 2′-5′P_3A_3 showed antiviral effect even if 2′-5′P_3A_3 was administrated 3-6 hr prior to vi...
Smith strain of murine cytomegalovirus (MCMV) was used for intraperitoneal infection of C57BL/6 inbred mice. in all three groups of high(10~(5.33)TCID_(50)), medium (2×10~(4.33)TCID_(50))and low (2×10~(3.33)TCID_(50)) dosage infected mice, MCMV infection was successfully established During 5-14 days after infection, in all dosage groups MCMV could be isolated from the spleens Starting from the 14th day after infection, salivary glands from all mice of the high and medium dosage groups were MCMV positive, wh...
Sc. inoculation of neonatal guinea pigs with guinea pig cytomegalovirus (GPCMV)resulted in acute infection of thymus. Infectious virus could be recovered from the thymus of infected animals at 5 days postinfection and the highest titers ef infections virus appeared at 10 days postinfection. The weights, total numbers of cells and T-rosette ferming cells were significantly lower in the thymus of GPCMV-infected animals tested on days 7 to 16, specially on day 10 postinfection as compared to control animals Al...
It had been carried out the detection of EHF-specific IgM and IgG for 265 continuous seral samples from 39 patients with EHF by means of reverse indirect ELISA test assay and IFAT. The results showed that the titer of IgM of severe patients was obviously higher than that of mild and moderate; whereas the titer of IgG showed on obvious difference. All of the cases together with2 IgM-negative were IgG-positive. And in olygouric phase the decrease of IgM was greater than IgG. As early as the 2nd and 3rd day of...
A study of viruses in domestic sewage of Shanghai County is reported. Adsorbtion-elution procedure is applied to concentrate the sewage sample, plaque assay is applied to count the virus number, microneutralization method is applied to identify the isolated viruses, TS test is applied to test the virus virulence. Viruses in the sewage range from 320 to 176000 pfuper liter, the maximum amout appears in fall They are mainly enteroviruses, most of them are vaccine strain.
150 stool samples were collected from acute infantile gastroenteritis patients in two hospitals in Shanghai from 1982 to 1984. The migration patterns of rotavirus RNA genome segments in polyacrylamide gel electrophoresis(PAGE)were compared by silver-staining. RNA PAGE showed that 74 samples were positive, among which 72 samples were of long electropherotype (2L), and 2 samples could not be categorized into 2S or 2L. Significant differences could be detected in some of the 2. 3. 7. 8 and 9 segments. Accordin...
Heliothis armigera polyhedrosis virus DNA was cleaved into 11 fragments with BamH1 which were isolated by using agarose gel electrophoresis. The resulting fragments and BamH1 digestion products of PBR_(322) DNA were used to recombine in vitro and to transform E. coli strain LE_(392). Based on the identification of colony hybridization and molecular weights of inserted fragments, we have obtained 11 recombinant plasmids inserting viral DNA, but two of molecular weights for the largest fragments are less than...
Enzyme-linked Immunosorbent Assay(ELISA) has a higher sensitivity, speciality and simplicity, in particular, it is convenient in large-scale observation. The water and soil in the environment which produced and used Heliothis armigera NPV inssecticide were detected by this assay. The contents of NaNPV were about 4-9PIB/g, 6-58PIB/g, 630PIB/ml water, above 830PIB/g, respectively in the farmland largely using the insecticide, the spread area, the heavily polluted pool and the product workshop.
A strain of bovine viral diarrhea virus (BVDV) was isolated from epidemic area of lamb in Inner Mongol in recent years During 1983-1985, we have deteted 229 shares of the fecal samples suffering from this illness in Inner Mongol, the shares of the viral samples account for 59 pereent.These virions appeared spherical and measured 40-80nm in diameter. Their surfaces have envelope structures. The virus can infect the lambs.22 passages virus have been acquired. The results of the complementary fixation test of ...
