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1987 Vol.02(2)

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Review

THE RELATIONSHIP OF THE EPSTEIN-BARR VIRUS-HOST BALANCE

YAO Qing-Yun, HUANG Mei-Ling

1987, 2(2): 1

RECENT DEVELOPMENT IN RESEARCH OF SATELLITE VIRUS AND SATELLITE RNA

KANG Liang-Yi

1987, 2(2): 7

COMPARATIVE STUDIES OF INFLUENZA A (H_3N_2) ISOLATED FROM PIG AND HUMAN Ⅱ CHARACTERIZATION OF BIOLOGICAL PROPERTIES

TIAN Mu-Zhen, LUO Qun-Ming, CHEN Shun-Lan, FENG Ling-Ling

1987, 2(2): 12

The influenza A viruses(H_3N_2)isolated from pigs each year were antigenically indistinguishable from contemporary human isolates in 1979-1982,the characteristics of biological properties were also similar.The results showed that haemagglutinins of pig strains were similar to human strains by being heat sentively,agglutining to the rang of animals red blood cells,sentively of pH etc. There were some differences between the strains in various years by agglutinated various animals red blood cells.The dove red...

PRELIMINARY STUDIES ON THE VIRUS ETIOLOGY OR CHILDREN S ACUTE MYOCARDITIS IN WUHAN

DONG Ji-Hua, TIAN Mu-Zhen

1987, 2(2): 20

This study was carried out from 1970-1980. Throat and feces samples for viral isolation were taken from acute children s myocarditis patients and antibodies to Coxsackie B_(1-6)viruses were mesured by a microneuralization test.Seven strains were isolated.They were 3 Adenoviruses,1. Coxsackie B_3 virus,1 Echo virus and 2 cnteroviruses.Moderate order associations were present for four patients because 3 Adonoviruses and Coxsackic B_3 virus were isolated from the feces and four fold increase in type specific n...

THE INHIBITION OF SODIUM HEXAMETAPHOSPHATE ON PHAGE INFECTION OF S. KANAMYCETICUS

CUI Han-Jun

1987, 2(2): 24

In order to produce an inhibitory effect on the infection of the strain by phage, though the phage was not inactivated,0.05-0.1% sodium hexametaphosphate,(NaPO_3)_6,was added to the fermentation medium of S kanamyceticus.Sodium hexametaphosphate did not affect the growth of S. kanamyceticus and the production of kanamycin. It did not interfere with the resin extraction of kanamycin either.

THE INTERFERON OF MICE WITH EXPERIMENTAL INFECTION OF MURINE CYTOMEGALOVIRUS

HU Chun-Da, WEN Yu-Mei

1987, 2(2): 29

C57BL/6 mice were infected intraperitoneally by murine cytomegalovirus (MCMV).Secretion of virus from salivary glands was positive up to 102 days.From 6 to 32 days after infection, no interferon(IFN)activity could be detected in all collectcd serum samples. However, spleen cells from these infected mice still responded to IFN inducers in vitro. All spleen cells produced comparable IFN to NDV and Con A induction as that of the control group,except that there was a transient decrease of IFN production to NDV ...

THE MORPHOLOGICAL MANIFASTATIONS OF THE CERVICAL TISSUE AND EXFOLIATED CELLS OF MICE INFECTED INTRAVAGINALLY WITH LIVING AND INACTIVED TYPE 2 HERPESVIRUSES.

MAN Yan-Gao, CHEN Min-Hui, HU Li-Ping, LEI Sen-Lin, CHEN Hong-Bin

1987, 2(2): 37

The living and inactived by U.V.type 2 herpes simplex viruses wer inoculated into the cervix of mice.The sequential cytological manifastations of vagina and cervix can be summarized as follows.1. The first visible light-microscopic change is the hypertrophy of the cytoplasm and nucleus of infected cells,this increase the cell in size.2. Many cells were fused to form a multinuclear cell3. The single. coarsely granular,acidophilic intranuclear inclusion was found in the infected cells4. Nucleoli and nuclear c...

EFFICACY OF HUMAN LEUKOCYTESINTERFERON IN CHRONIC HEPATITIS B

WU Zhen-Ou, GUAN Mei-Ying, LIN Sheng-Qing, WU Zhang-Qi

1987, 2(2): 45

Seventy-three cases with chronic hepatitis B were randamly divided into two groups,among which 35 cases were treated with interferon of human leukocytes,and 38 cases were treated with poly I:C.With several cases as the control group,the study group received interferon of human leukocytes 40000μ3/week I. M.for 3 months;and the control group was treated with poly I:C 2ml 3/week I.M.for 3 monthsThe side effect in the study group was of same manifestation:two cases with slight fever.The results showed that the ...

DETECTION OF VIRUSES IN RAW WATER OF WATER PLANT AND TAP WATER

JIANG Hui-Hui, YAN Hui-Qin, LI Jie, HONG Dao, YUE Shun-Lin, MEI Ye-Xin

1987, 2(2): 50

This paper reports the results of detecting viruses in the raw water at the inlet of water plant and in the output tap water by modified Farrha′s Method. The amount of viruses in the raw water reaches 4-50 pfu/10L in different seasons. Most of the viruses are enteroviruses, mainly polio viruses. Test of ret/40 shows that 3 strains might be of wild type,whereas the others are not.Since no virus has been found in 40L of tap water, the virus standard in drinking water(no virus in 10L of samples),designed by WH...

THE INACTIVATION OF POLIO VIRUS BY CHLORINATION IN WATER PLANT

YAN Hui-Qin, JIANG Hui-Hui, LI Jie, HONG Tao, YUE Shun-Lin, TUN Jin-Wu, HE Wei-Qin, WU Mei

1987, 2(2): 55

In this experiment attempt was made to determine the efficiency of inactivation of enterovirus by the chlorination in water plantCertain amount of polio virus I(LSc) was added to the chlorinated water samples taken from the plant. The total residual chlorine, free chlorine,and the polio virus in suspension was determined every 30 minutes.The first one of two chlorination procedures is the major step for inactivating virus in water plant.After 60 minutes contact with chlorine,no survival virus can be found i...

BIOASSAY OF A NUCLEAR POLYHEDROSIS VIRUS OF THE TEA LOOPER, BUZURA SUPPRESSARIA, PROPAGATED IN CELLS GROWN IN VITRO

ZHANG Zhong-Xin, BANG Hui-Yin, JIN Feng, ZHANG Yang-Lian, WANG Lu-Ming, ZENG Yun-Tian

1987, 2(2): 60

Bioassay of a Nuclear Polyhedrosis Virus propagated either in vivo or vitro was conducted on third-instar Buzura suppressaria larvae which were reared on an artificial diet contaminated with virus, The regression equation between dosage and mortality were:Y=3.1815+0.697XY=3.2000+0.665XThe LD_(50) value and 95% fiducial limits were 4.0753×10~2 (9.0675×10~2)(1.7361×10~2)PIB/g of diet and 5.0898×10~2(1.2411×10~3)(2.0874×10~2) PIB/g of diet,respectively.The LT_(50) value for 4×10~5PIB/g of diet were 4.7168 days...

RELATIONSHIP BETWEEN THE CONCENTRATION OF SOYBEAN MOSAIC VIRUS IN SOYBEAN AND SEED TRANSMISSION RATE OF THE VIRUS

GE Shen, ZHANG Ming-Hou

1987, 2(2): 66

The concentration of soybean mosaic virus in soybean leaves,immature seeds,flowers and non-coated mature seeds of 6 soybean cnltivars were tested by local lesion assay or ELISA,and the relationships between virus concentrations in these parts and seed transmission rates of the virus were analysed.Results showed that the concentration of SMV in soybean leaves and immature seeds varied significantly with soybean cultivars and the days after inoculation. No difference of SMV concentrations in flowers among six...

A PRELIMINARY IDENTIFICATION OF THE CAUSAL VIRUSES OF GARLIC MOSAIC DISEASE IN GUANGDONG PROVINCE

ZHAO Shun-Qiang, FAN Huai-Zhong, GAO Jiao-Wan, TANG Wei-Wen

1987, 2(2): 75

Seven garlic cultivars in Guangdong province were found 100% infected by mosaic disease. The infected garlics showed typical symptoms of mosaic,yellowstreak, curly-top and dwarf. The viruses were transmissible from garlic to garlic by juice, but other 35 tested plant species were found insusceptibleTwo kinds of flexible virus particles with the sizes of 12-13×500-700 nm and 12×900-2000nm respectively,one kind of rod-shaped particle with the size of 14×200-300nm, and a kind of pinwheel inclusion existing in ...

A NEW STATISTIC METHOD USED TO ANALYSIS SERIAL COUNT DATA IN MEDICAL AND BIOLOGICAL EXPERIMENT

CHEN Dong-E, DONG Chang-Yuan

1987, 2(2): 87