1987 Vol.02(3)
This paper describes an improved fluorescent antibody technique for the detection of HAV. The assay is unique in its use of the characteristic of a highaffinity complex between fluorescence-conjugated avidin and biotin-linked anti-HAV to increase sensitivity. We have made a comparison between direct-fluorescent and fluorescentantibody technique with biotin-avidin with same lot IgG antibody and same lot ACMK inoculated HAV. Because of the high binding affinity of this system due to multiple binding of biotin...
This paper reported 42 cases with chronic hepatitis B and the virus carriers, whose ages range from 16 to 44,and the course of disease varies from half a year to 11 years. The patients were divided arbitrarily into 3 groups. There were 10 patients in the first group, who were treated by interferon alone, 60000u intramuseularly, 3 times per week and 3 months course of treatment. There were 3 cases with HBeAg-negative accompanied by anti-HBepositive,1 case of HBcAg,DNAP and HBV-DNA negative respectively. In ...
In the present study we have first identified the factors regulating the formation of plaques by herpes simplex virus (type 1) infecting the primary muscle and skin monolayer cells of human embryo. The numbers of plaques formed in the two varieties of viral diluted solutions(e.g., Hanks solution containing 2％ fetal bovine serum and/or 199 solution)are parellel to each other. In the diluted soluticn 0.5ml/bottle with a pH 7.0 appear more plaques, whereas plaques are most abundant in the case of adhered viru...
A new immunoenzymatic assay was established with avidin labelled peroxidase(A-P) and biotin labelled anti-HBc(B-Anti-HBc)for deteeting IgM antibody to hepatitis B core antigen. A-P, B-Anti-HBc and antiserum to human μ chain for the assay were prepared by our laboratory, Aggregated normal rabbit IgG was added to the buffer solution employed as a dilution to overcome the influence of rheumatoid factor of the blood specimens The specificity of the method was proved by SPA absorption method and anti-HBc inhibit...
Human cytomegalovirus(HCMV)latency model in human embryonic foreskin cells(HEF)was established with 500ng/ml of Tunicamycin(TM) which is a glucosamine-containing antibiotic that specifically inhibits glycosylation of proteins. Toxic effects in confluent monolayer cultures occurred when more than 500ng/ml of TM was used. The different concentrations of TM reduced the yield of HCMV to varying degrees, however, HCMV was not totally inhibited. At 5 days postinfection HCMV was not detectable at a TM concentratio...
Using a micro-ELISA technique, the serum specific IgG and IgM antibody of the EHF patients were measured sequencially as a part of a doubleblind, placebo-controlled clinical trial of ribavirin therapeutic efficacy in the treatment of EHF. The results showed a significant decrease of antibody levels in the ribavirin-treated group (p<0.001 in IgG, p<0.05 in IgM). It suggests that ribavirin has an inhibition effect on the EHF patients antibody responses. Therefore it may reduce the formation of the circ...
Eight stool samples of acute diarrhea obtained from the new-born baby wards, the Shanghai First people s Hospital were investigated by ELISA and RNA PAGE silver-staining method. Three samples are rotavirus specific-antigen positive, and three samples could offer enough RNA to analyse. Antibody titer of convalescent serum of six patients were higher than 1280, four times as high as that of control groups The result showed that the epidemic of acute new-born baby diarrhea was caused by rotavirus, and the migr...
Potentiation of interferon s antiviral activity was determined by replication inhibition of vesicular stomatitis virus in human lung cancer calls(A549). Combined preparations of human IFN-α and IFN-γ interacted with cells synergistically to cause a much grearer level of protection than that expected on the basis of their additive or multiplicative effect. The potentiation was not the artifact of an accelerated development of IFN-γ-induced antiviral resistance in the presence of IFN-α. The rest components in...
The paper confirmed the presence of alkaline protease in the nuclear polyhedra of the Attacus ricini and the characteristics of the protease. When the nuclear polyhedra was exposed to alkaline solution, a proteolytic enzyme in the polyhedra degraded the major structural proteins of polyhedra into proteins with the lower molecular weight, The proteolytic activity showed an optimum temperature of 40℃ and an optimum pH of 11 to 12. The activity was inhibited by metallic ions such as Hg~(2+) and Cu~(2+). The en...
The AsGV(Agrotis segetum granulosis virus)insecticide has been used to control Agrolis segetum schiffermuller on the Chinese Winter Cabbage and corn. Comparing with contrast areas, the number of the insect pests reduced more than 90% in control areas with AsCV insecticide 40g(virus content 660mg)per mu; the Chinese Winter Cabbage yield increased 1200kg per mu; the corn yield increased 150kg per mu.
In this study the local lesion host(Top Crop, a variety of bean)was used to test the percentage of soybean mosaic virus(SMV) infection. The results showed that the infection rate(%) in mature seeds is as high as that in the seedlings having resulted in those infected seeds. High percentage of SMV infection was detected in all parts of immature seeds and green pods. While in mature seeds, the infectious SMV was only detected in the parts of cotyledons and embryos,However,this high percentage of SMV infection...
Four varieties of citrus infected with Citrus Infectious Variegation Virus(CVV) were freed from the virus by shoot-tip grafting in vitro. The tests carried out by enzyme-linked immunosorbent assay(ELISA) were faster and more sensitive than those made by graft-inoculation to indicator plants. The survival rate of grafting was up to 85-90% if shoot-tip and cut of rootstock were treated with 6-benylaminopurine(BAP) or 2, 4-D before grafting.
Four strains of actinophages φSK1, φSK2, φSK3 and φSK4 were isolated from aberrant fermentation fluids of S. kanamyceticus obtained from the Fourth Pharmaceutical Factory in Chengdu. It was demonstrated that they differed from each other. but belonged to the same serological properties. They also showed some different characteristics from some other strains isolated in different areas in China and Japan.
The present paper deals with the phage technique to determine the Pseudomonas solanacearum bacteria quantitatively, Results show that mean recovery of pure bacteria cells was 81% at concentration of 10~3 colony formation unit(CFU)per ml of phage Zp-2 isolate. The optimun adsorption temperature of Zp-2 is 30℃. and optimun adsorption time is 30min. It is a high sensitive method for detecting Pseudomonas solanacearum.
Chemical properties of newly isolated Bombyx densonucleosis virus(BmDNV), Yamanashi isolate(Y-DNV), were found to be different from those of first isolated BmDNV, Ina isolate. The viral DNA of Y-DNV was resolved in an agarose gel electropherogram into two closely migrating bands, DNA Ⅰ and Ⅱ. The digests of these DNAs treated with some restriction enzymes differed clearly from each other. In order to clarify this phenomenon, the structure of these DNA molecules was investigated by electron microscopy. Both ...
