1987 Vol.02(4)
A 30,000-dalton structural protein(VP30)on herpes simplex virus type-2 (HSV-2) -infected BHK cell membranes was purified with an immunoadsorbent column consisting of the HSV-2 type-specific monoclonal antibody(McAb) CH-A9 coupled to Sepharose 4B. The Evidence shows that the VP30 has some properties as follows: First, its molecular weight determined by sodium dodecyl sufate-polyacrylamide gel electrophoresis(SDS-PAGE)is 30,000. Second, the structural protein is HSV-2 type-specific antigen showed by indirect ...
We have modified and improved the method of immune adherence hemagglutination (IAHA) test and used it to detect virus antigen and antibody of epidemic hemorrhagic fever (EHF). We compared the IAHA titers of different strains infected in Vero-E_6 cells The IAHA titer of A_9 strain showed to be the highest (1: 64) among the three strains. 9 EHF patients paired sera, 3 con valecant sera and 6 immunized rabbits sera were detected with IAHA and compared with IFA. The positive rates detected by the two tests we...
Ten patients with high titer HBsAg of chronic hepatitis B and carriers were treated with aeyclovir and compared with inosine and so on. After one year s follow-up, six cases were treated with intermittent therapy, three patients HBsAg and DNA-P became negative at the sixth month, two began to rise after nine months, the viral markers in 5 cases were similar to pretreatment, HBV-DNA were positive in four cases, however, all viral markers (including HBV-DNA)were negative in one case, suggesting that spontane...
The variables which affect the qualitative and quantitative aspects of rubella virus hemagglutination were defined. Large amount of high titered hemagglutinin was obtained by replacing the supernatant of rubella virus infected BHK_21 cells with fresh medium daily. The optimal result wasobtaitied by using Auletta s buffer, pH6.2. Kaolin and Heparin-MnCl_2 were employed to the treatment of serum samples in hemagglutination inhibition test. Among the 107 serum samples tested, the agreement was 98％. Kaolin trea...
The anti-JEV McAb is tried out to treat the mice and goats which have infected by JEV,Tbc results show that the JEV-McAbs have an excellent curative effect to several JEV-infeeted mice.It is evidently better than that of the PeAb. After 120 hours of infection, most of the mice have already had the signs of JE,some were nearly dead. If McAb or their F(ab )_2 were given even at this time, approximately half of the mice could be saved from JE. M-MeAb has an evident protective effect in the goats too. After 48 ...
In this paper, followed-up observation during 6-20 months was taken on 40 cases with chronic hepatitis B, who have been treated with small dose interferon in a period of 6 months. Observation from the further effect of the negative percentage of HBV marker showed the results as follows: 12 of 19 HBeAg positive cases turned into negative(63.1％), anti-HBe positive appeared in6cases; 2 of 3 cases with DNA-P positive became negative(66.6％),2 cases with HBcAg positive became negative 1 of 3 cases with HBV-DNA po...
The brains and lungs of mice Balb/c infected by standard virus H8278 are examined by electronmicroscopy. There are viruses in the cells of the lungs and brains. The viruses show round and ellipse in shape and the structure of cell membrance is clear, in which loase threadlike or granule structures are included. Foot process could be seen outside of the cell membrance. The sizes of viruses are between —120nm. Inclusion bodies are found in the cell plasma.
With MC-1 type microcarrier at concentration of 5mg/ml, the mouse L929 cell line could attach and proliferate on them very well if the stirring speed was lower than 50r/m.When the cell density was inoculated about 30×10~4/ml,it could proliferate to 1×10~6/ml after 3 days generally. At this time,priming with 25 IU/ml mouse IFN about 12-24 h and then induced with NDV and superinduced with cycloheximide(20μg/ml) and actinomycin D(2μg/ml),the titer of IFN in the media would be 10~5IU/ml approximately, or 10~5IU...
The present paper deseribes the bacteriology detection of the different preparations and standard samples of the insecticide of Buzura suppressaria nuclear polyhedrosis virus. Pathogenic bacteria, such as Shigella, Salmonella,Yersinia, Bacillus, Vibric, Escherichia coli and Micrococcus were cultured without isolation. Mice infected by different preparations via oral cavity and injection revealed symptoms on the epithelial surface and the pathologic changes in the viscera. The results show that the insectici...
A virus isolate-Wc has been obtained from diseased white clover which grows in the campus of Northwestern Agricultural University. The Wc isolate only gives necrotic local lesions on bean and cowpea without systematic spread, but systematic on tobacco. The thermal death point is between 50—55℃. The life in vitro is 12—24 hours and the dilution end point is 10~(-2)—10~(-3).Under EM, partially purified virus preparation presents five components with uniform width(18—20nm) but different in length (58, 52, 41, ...
For the purpose of obtaining attenuated strain of TMV, pure virus isolated from tomato plants with mild symptom were treated with nitrous acid for 30, 40, 50, 60 minutes, as a result, 30 symptomless mutants had been obtained. In cross protection tests on tomato, the attenuated virus DN60-3 interfered more strongly with the severe mosaic strain, the streak strain and the necrosis strain occurred in Jinan (Shandong), whereas interfered weakly with the yellow mosaic strain and the yellow ringspot strain, both ...
The substrate, p-nitrophenolphosphate disodium (PNPP), of alkaline phosphatase has been commonly used in enzyme linked immunosorbent assay (ELISA).The fluorescent substrate, 4-methylumbelliferal phosphate, usedin the indirect ELISA procedure(FS-ELISA)was compared with the substrate PNPP used in same indirect ELISA procedure. The fluorescent reaction could be observed under the UV-light at 253.7nm emission wavelength when coating was done with 0.1ml suspension of cucumber mosaic virus at the minimum concentr...
The present paper deals with the phaga technique to quantitative determinate the survival of causing bacteria of ginger wilt(Pseudomonas solanacearum)in soil. Results show that the bacteria in inoculated soil could recover to 57.4％ at 10~3 CFU/g soil. The living bacterial cells,even at 10~2CFU/g soil,could be detected. The phage technique is a sensitive, rapid and simple method.
An outbreak of acute nonbacterial diarrhea ocurred among the newborn calves of a dairy in Hefei. A strain of bovine rotavirus was isolated using MA104 cells. The isolated virus appeared as typical rotavirus by electronmicroscopy, From the third passage it showed granulated fluorescence in the cytoplasm of MA104 cells and produced cytopathogenic effects(CPE).The RNA PAGE patterns of new isolated virus were indistinguishable from that of reference NCDV strain of bovine rotavirus. The new virus was identified ...
The ELISA method was applied to assay HSV antibody in vaginal secretions from women with and without cervical cancer, The prevalence (85.7％)and geometric titers (57.98) of HSV-2 antibody in vaginal secretions from those with cervical cancer were significantly higher. The results indicated that the cervical cancer might be related to a high incidence of HSV-2 infection.
