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1989 Vol.04(3)

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Review

Virus Infections and Virus Receptors

LI Ming-Yuan

1989, 4(3): 219

The Advances of Researches in Bee Paralysis Virus

FENG Feng, CHEN Shu-Jing, YIN Ming-Biao

1989, 4(3): 227

Experimental Acute Coxsackle B-3 Viral Myocarditis in Mice

YANG Ying-Zhen, JIN Pei-Ying, GUO Qi, YANG Xue-Yi, PU Shou-Yue, CHEN Hao-Zhu, YANG Jun-Hua, WANG Ke-Jiang, SHI Jian-Ying, GONG Zu-Xun, CHEN Ju-Yang, PENG Bao-Zhen

1989, 4(3): 233

An experimental acute myocarditis caused hy Coxsaekie B-3 virus(CB_3V)was developed in male BALB/c mice 4 weeks of age. The animals were szc-rificed at the 7th day after infection, After gross examination, the heart was cut transversely into 3 pieces for light, electron microscopic examinations andvirus assay. In addition, myocardial lesions were also quantitatively measuredby image analysis. White spots, streaks or patches were seen on the surface of ventrieles ofhearts in the infected group (14 mice) .The...

Study on Vector Homology in Clinical Specimens with Cloned Probes

WU Xin-Xing, YANG Ping, ZHAO Wen-Xian, LIU Wei-Qian, TAN Yun

1989, 4(3): 239

Nucleic acid hybridization techniques using cloned probes (recombinantplasmids composed of viral fragment and bacterial plasmid vector, such as pBR322)are applied in assay of clinical specimen in research and diagnostic laborato-ries. They have simple, quick and sensitive adventages, and help to detectthose virus gene that are difficult or impossible to culture. However, it should be noted that there may be a homology problem between cloued probe and cli-nical specimens. We detected 213 specimens from 173 p...

Cloning and ldentification of the Type-specific DNA Fragment of Herpes Simplex Virus Type 2

CHU Yong-Lie, FANG Yi-Lan, LIU Yan-Na, ZHOU Yu-Ling

1989, 4(3): 245

The DNA of Herpes Simplex Virus type 2 (HSV-2)digested with restrictionenzyme BamHI and the BamHI G fragment was isolated, which locates in thereverse repeat sequence and spans the junction site of L and S component.Then the BamHI G was inserted on the BamHI cut-site of the vector plasmidpUC 8. In order to modify this recombinant plasmid, the EcoRI--KPN I fra-gment of the recombinant plasmid was removed by restriction enzyme digestionwith EcoRI and KPN 1. After modification of the ends and ligation of them,...

A Rapid lgM Antibody Capture ELISA and its Employ in the Early Diagnosis of Japanese Encephalitis

ZHANG Ming-Jie, HONG Mei-Xian, JIANG Chao-Zhun, MA Wen-Yu, YU Bi-Yun

1989, 4(3): 251

A rapid IgM antibody capture (RMAC) ELISA using enzyme labeled one ofthe species specific McAb 2H_4 as tracer was studied for the early diagnosis.The antibody thus obtained was confirmed specific, because it was destructableby 2-ME, could be blocked by JEV antigen, and had no cross reactivity with HFRS virus or adenovirus IgM antibody positive sera, as well as was notinterfered by RF. 10 serum specimens were tested for JEV-IgM by conventionalMAC-ELISA and RMAC-ELISA simultaneously, and the Iatter one was mo...

observation on the Efficacy of Hepatstis B Vaccine Combined lmmune with BCG and DPT

JU Hai, ZHAO Wen-BIn, HE Tian-Sheng, GUO Ren-You, YANG Hou-Xian, GU Xiang-Yun, ZHANG Xiu-Hui, LI Hua, LIU Shu-Hua, HU Cai-Ru, WEI Ai-Lian

1989, 4(3): 256

In order to study the immune response of HB vaccine combined with BCGand DTP, 150 newborns of HBsAg negative. mothers were divided into threegroups and observed. Babies in groups 1 and 2 were given injection of 10μg,20μg HB vaccine respectively, group 3 had no HB vaccination. But all thethree group were given BCG and DTP by immune plan.The babies in group 1 and 2 whose anti-HBs reached the level of P/N≥5were 93.18% and 89.58% respectively. There is no statistical difference forthe two groups (P>0. 05). M...

Preliminary observation of the Inhibitory Effect of (s) DHPA on the Infection of EHFV in Suckling Mice

YAN Xiao, XIONG Hong-En

1989, 4(3): 262

The inhibitory effect of (s)-9-(2, 3-dihydroxypropyl) adenine ((s)-DHPA)on infection of Epidemic Hemorrhagic Fever Virus (EHFV) J10 strain in su-ckling mice was observed. The experimental results showed that the morbidityrate (44.44 % and 55.17%) and infection rate (44.44% and 55.17%) of two(s)-DHPA treatment groups were markedly lower than that of the control group(83.33% and 86.96% respectively). Their differences were statistically signi-ficant. These results suggested that EHFV replication in suckling m...

Replication of HFRS Virus in Cultured Human Lymphoblastoid Cells with B Cell Characteristics

YAO Zhi-Qiang, YANG Wei-Song, LIU Jian, ZHANG Wen-Bin, BAI Xue-Fan, LI Xue-Rong, HUANG Mei

1989, 4(3): 266

The susceptibility of human B lymphoblastoid cells to HFRS virus infectionwas studied. The results showed that the cells were productively infected byHFRS virus. The persistently infected cells had no obvious cytopathic effects andwere indistinguishable morphologically from uninfected cells. It was difficult toidentify complete virions in infected lymphoblasts by electron microscopy con-trasted with strong immunofluorescence staining for intracellular HFRS virusantigens observed in the majority of these cel...

Studies on the Small Round Virus--like Agent of Causing Neonatal Acute Gastroenteritis

LI Xiao-Mian, REN Zhong-Yuan, ZI Zi-Jiang, ZHOU Hong

1989, 4(3): 271

Out of 33 neonates with acute gastroenteritis, 12(36.4%) were detected tohave virus-like particles by electron microscopy once agian. The small roundvirus-like agent(SRV)was found in 11(33.3%)samples. In one sample other vi-rus-like particle was found. The SRV was confirmed to cause this neonatalacute gastroenteritis by ultrathin section and immune electron microscopy (IEM).The SRV was measured 26 nm in diameter and showed fine spiky structureon the surface. The buoyant density of SRV was determined to be 1...

In Vitro Induction of SRBC-sgecific Antihody by Human Tonsillar Lymphocytes and the Effects of HSV-1 Infection on it

WANG Ming-Lun, ZHOU Yao-Xi, JIN Jin-Ren

1989, 4(3): 278

We established a stable human tonsillar cell culture-immunization systemin vitro, in which sheep red blood cells (SRBC), a virus-irrelevant antigen,induced efficiently specific antibody production. In contrast to traditional con-cepts, we demonstrated that adhering cells (mainly macrophages) in tonsilsplayed a suppressive role in induction of specific antibody response in vitro.Depletion of adhering cells increased significantly the production of this anti-body. We also found the SRBC co-stimulated with sui...

The Detection of Potato Leaf--roll Virus by the Microslide Gel Double Diffusion Test

HOU Yan-Jun, ZHANG He-Ling

1989, 4(3): 292

The microslide gel double diffusion test was used to detect potato leaf-rollvirus (PLRV). It was found to be sufficiently sensitive for the detection ofPLRV in extracts of infected plants. A 1: 4 dilution of extract of PLRV infectedpotato stem could be detected unambiguously. The sensitivity of microslidewas compared with that of the Ouchterlony plate technique. The microslide te-chnique was capable of detecting 1 μg/ml of purified PLRV. This sensitivitywas sixfold as much as that of the Ouchterlony plate t...

Some Properties of Cell Suspension Cultures Containinq Citrus Exocortis Viroid

LIU Yu, DIAO Hong-Yan, ZHOU Yong-Zhi, CHEN Ya-Li, DING Da-Ming

1989, 4(3): 297

Gynura aurantiaca D. C. i the sensitive indicator plant of citrus exoc-ortis viroid (CEV). Cell suspension cultures from healthy and CEV-infectedGynura aurantiaca D. C. have been established. Some properties of the cellsuspension cultures have been described by means of growth, pH and temperaturecurves respectively. CEV cln replicate in cell suspension cultures. The cons-istent detection of CEV and host nucleic acids, through successive subcultures,suggest that the amplification of CEV inhibit the replicati...

Protection Effecf of CMV--satellite RNA Biological Control Agent on Productive Tobacco in the Field

CUI Yong-Han, HE Ming-Hua, HAN Zhen-Shu, KANG Liang-Yi, MOU Gu-Sui, ZHANG Xiu-Hua, TIAN Bo

1989, 4(3): 304

Satellite contained CMV, the biological control agent, was applied on pro-ductive tobacco variety in field to control diseases caused by CMV. Incidenceof the treated plants decreased 84.9--86.4%, harvest time showed 5 daysearly, high quality tobacco incresed 151.3--173.5%, and income in a unitarea increased 43.3--65.7%. Moreover, the biological control agent was foundto improve tobacco resistance to fungal infection.

Studies on Pathogens of Sesame Virus Disease I. Identification of Sesame Dwarf Necrosis Disease

YANG Shu-Jun, YU Zi-Lin, XU Ze-Yong

1989, 4(3): 308

A virus was isolated from diseased sesame (Sesamum indicum) plantwith symptom of dwarf crinkle necrosis both on leaves and stems in Wuchang,Hubei in 1986.The plants of 43 species (cultivars) among 8 families were inoculated withthe virus isolate which can infect those of 14 species involved in 7 families,for instance, infecting Chenopodium amaranticolor, C. oinoa, Gomphrenaglobosa locally and Brassica campestris, Zinnia elegans systemically. My- zus persicae and Aphis craccivora transmitted the isolate in n...

Growth Characteristics and High Titer Culture of Grass Carp Hemorrhage Virus (GCHV)-873 in Vitro

FANG Qin, KE Li-Hua, CAI Yi-Quan

1989, 4(3): 315

Some biological properties of Grass Carp Hemorrhage Virus (GCHV)-873have been studied in this report. This virus could make grass carp kidney cells(CIK) to produce a unique cytopathic effect. At 28℃ its logarithmic growthphase occured 12 to 48 hr after infection. The virus titer reached 1.78×10~(11)PFU/mlwhen the MOI was approximately 0.05PFU/cell. There were slight differencesin virus titer under incubation of 25℃, 28℃and 30℃ respectively, and thesimilar results in 5% CO_2 or in normal atmosphere.

Study on Small Dosages of HulFN--α (Le) Accompanied with Poly 1: C in the Treatment of Chronic Active Hepatitis

GUO Tong-Xiang, DUAN Fa-Yu, WANG Ya-Zhen, TUN Chang-Jing, LIANG Jing-Wen, GENG Wen-Ju, XUAN Xiang-Chan

1989, 4(3): 320

The effectiveness of small dosages of interferon-α accompanied with PolyI: C in the treatment of chronic active hepatitis B is reported in this paper.40 cases of chronic active hepatitis B were rondomly divided into study groupand control group, 20 cases in each. The study group was treated with interfe-Ton-α(30,000 Uqd ) and poly I: C (2mg q 3 days) for 3 months, while control group was given symptomatic and supportive therapy only. The statusof HBsAg, HBeAg, Anti-HBs, Anti-HBe, Anti-HBc, HBV-DNA, TTT andS...

Studies on the Nuclear Polyhedrosis Virus lsolated from the Mulberry Caterpillar Sarcopolia illoba (Butler)

WANG Yi, ZHANG Lv-Hong

1989, 4(3): 325

This paper reports the results of studies on a Nuclear Polyhedrosis Virus(SiNPV) isolated from the mulberry caterpillar [Sacopolia illoba (Butler)]in Harbin. In most cases there were 4--5 nucleooapsids enveloped within eachvirus bundle of SiNPV. The polyhedra of SiNPV were 2. 06±0. 27μm in diameter,and nucleocapsids were measured about (0.36±0.01)×(0.058±0. 004)μm. SiNPVwas also infectious to the neonate larvae of several other noctuids includingDiscestra stigmosa, Mamestra brassicae, Scotia segetum, S. ips...