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1989 Vol.04(4)

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Review

Advance in Research of Barley Stripe Mosaic Virus

PENG Hua-Xian, QU Shi-Mian

1989, 4(4): 329

An Investigation on the Vertical Transmission of EHFV among the Mice in the Endemic Area

LIU Jiang-Qiu, LI Zhong-Xi, SHEN Bao-Jun, CA Zeng-Lin, JIN Xian-Tao, WEN Qing-Li, CUI Wei, CHEN Ting-Cai, HE Yi-Xiang, ZHANG Xin-Wang

1989, 4(4): 336

The vertical transmission of epidemic haemorrhagic fever virus (EHFV) in mice has not been confirmed. From 1985 to 1986, We conducted the studies in epidemic fiels for getting a reliable answer to the above problem on the basis of a serial sludies in the laboratory. In our study IFA was adopted to detect the EHFV antibody and antigen; tissue culture technique was adopted to isolate EHFV from fetuses which were dissected from the uteri of infected mice. Our results are as follows : In the field study, the sp...

The Observation on the Specific Type of Intranuclear Inclusionoid Body Induced by Adenovirus infection Under Electron Microscope

GAO Jun-Feng, LI Zhong-Qin, JING Guan-Hua, SONG Ai-Zhi

1989, 4(4): 341

A specific type of inclusionoid body is observed in the virus crystalline within the infection nucleus of a clone of type 4 virus. The inclusionoid body shows hollow-core shaped long tube. Its length may reach 1.5μ and its outer diameter is 70nm, inner diameter 20nm. This morphoiogic structure may be considered being related to formation and development of virus capsid.

The Study on Antiviral Effect of Methanol Extract of Rubia cordifolia against Herpes Simplex Virus 2 in vitro

JIN Yu-Huai, WANG Yu-Kun, GU Bao-Liang

1989, 4(4): 345

Rubia cordifolia is a traditional Chinese drug. In this paper, it was reported that the methanol extract of this drug showed significant antiviral effect against herpes simplex virus 2 in vitro. In Vero cell line, virus inhibiting index reached 3 log. Virus reproduction inhibiting test showed that the reproduction of infectious virus parlicles was inhibited. By comparing the antiviral effect of this agent in different cell line, it was found that the antiviral effect of this extract was cell line dependent.

The Purifications of Mouse Ascitic Tumor Virus(SRSV) and Viral Ribonucleic Acid(SRSV RNA)and Investigation of Some Properties

CHAN Yi-Fei, JI Hong, WU Zheng-Quan, LV Mei-Zhen, ZHENG Bao-Fen, CHENG Li, KONG Xian-Shou

1989, 4(4): 350

SRSV was obtained from the supernatant of infected NIH 3T3 mouse fiblasts and purified by sucrose density gradient centrif ugation.SRSV RNA was prepared from fresh viral pellets and extracted with chloroform-phen(?)l and fractionated on sucrose gradients. The RNAs of various fractions were determined by sedimeptation coefficient(s) and followed by gel electrophoresis. The component of SRSV RNA contains two main fractions, one sedimenting at about 60-70s(after treatment with heat at about 35S), plus small am...

Molecular Epidemiology of Human Rotavirus in Ten Cities of Shandong Province in China

WANG Ying-Cai, SHAO Ji-Jun, ZANG Yun-Juan

1989, 4(4): 359

Using the PAGE method, we analysed the electrophoretic types of RNA rotavirus obtained from 170 stool samples with infantile diarrhea from ten cities in Shandao from 1985—1986, In 3 cities subgroup Ⅰ rotavirus was found to be the main etiologic agent, subgroup Ⅱ in other 3 cities and both subgroups were found prevailing in the rest cites. Among 34 different electrophoretic typs observed, 14 belong to subgroup Ⅰ and 20 to subgroup Ⅱ。In the cities with two subgroups co-prevailing, there were far more electrop...

Detection of Dengue Virus RNA by DNA-RNA Dot-spot Hybridization

CHEN Huo-Sheng, GUO Hui-Yu, LUO Jin-Xian, HU Jin-Xin

1989, 4(4): 365

Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA. Using the [a-~(32)p] dCTP labelled pEH 920 DNA as the probe, dengue virus RNA in the supernatants of infected C6/36 mosquito cells have been detected by DNA-RNA dot-spot hybridization. The results show that the probe strongly reacted with dengue 2 virus RNA though it also cross-hybridized with dengue 1, 3 and 4 virus RNA. The preliminary experiments show that the probe can at least de...

Studies on Infection of Epidemic Hemorrhagic Fever Virus (EHFV) on Suckling-young Pigs

ZENG Gui-Jin, XIA Tie-Guo, ZHAO Yun-Shi, ZHAO Dong-Yan, FU Zhan-Guo

1989, 4(4): 370

This study confirmed that suckling young pig is a laboratory animal susceptible to EHFV strains R22 and HB55 isolated from wild rodents and patients with EHF. The study showed that EHFV was able to propagate in many tissues of pigs, Viremia appeared in suckling young pigs on 6—9th days after inoculation of EHFV. Specific fluorescence for EHFV antigen was detected in many tissues, especially in spleen and lung on 7—11th and 7—20th day of experimental infection. Pigs infected with EHFV developed high titre an...

The Contrast Observation of Antiviral Effect of Interferon with Two Different Dosages in Chronic Hepatitis B

HUANG Hua-Fang, XIONG Kai-Jun, WANG Xin-He, CENG Lin-Lan, LI Yuan, HE Yong-Wen, YANG Ze-Chuan, LING Mei-Xia, FAN Jia-Zhen, TUN Zhang-Qi

1989, 4(4): 383

In this paper, the recent and followed-up contrast observation was taken on 44 cases with chronic hepatitis B, who had been treated with two different dosages of interferon. There were 23 cases in group Ⅰ and 21 cases in group Ⅱ. The dosage of group Ⅰ was 1×10~6 iu im 3/w x 4 weeks, then returned to 1×10~6 iu im 2/w x 6 weeks. The total course was 10 weeks. The dosage of group Ⅱ was 6×10~4 iu im qod. The total course was 6 months. When the course of treatment was finished, the recent effect which reflected ...

Ultrastructure of Fat Body Cells of Leucoma selicis Infected with a Nuclear Polyhedrosis Virus

LI Wei-Qi, WU Er-Ni-Sha, TUN Zu-Yin, CHEN Lu

1989, 4(4): 390

The assembly processes of the Leucoma selicis NPV are observed by electron microscopy of sections of infected larval fat body cells. The synthesis and envelopment of the virus nucleocapsid and stages in the formation of the polyhedral inclusion bodies are described. Bundles of fibrous material were seen in the nuclei and cytoplasm of cells contaning polyhedral bodies. We also found that in the same polyhedron there are single and multiply embedded virus rods.

Electromicroscopic Observation of Bombyx mori Densonucleosis Virus Infecting Insect Cell Lines

ZOU Shuai-Zhou, XIE Tian-En

1989, 4(4): 396

There have not been formally reports of Bombyx mori densonucleosis virus infccting insect cell lines. In the present paper three insect overy cells of B.suppressaria, S. frugiperda and M.brassicae infected with Bombyx mori densonucleosis virus.The result is that cytopathic effect was only observed in the overy cells of B.suppressaria. The ultrastructural changes of the infected cells have been observed both in the cytoplasm and nucleus. At an early stage of infection, the infected nucleus hypertrophied grad...

Characterization of Clonal Strains from the Buzura suppressaria Ovary Cell Line Bs-484

LIANG Bu-Feng, LIANG Li, JU Shuai-Zhou

1989, 4(4): 402

Four clones (Bs-484 B,E, F, G) were successfully isolated from the established cell line of Bs-484 by the method of finite dilution. The growth characteristics of the clonal strains were different from the parent line. The cloned cell strains were different in their predominant morphology, cell doubling times and the relative ability to support replication of the nuclear polyhedrosis virus of Buzura suppressaria (BsNPV). The origin of the cloned cell strains was confirmed by comparing their isozyme profiles...

Studies on the Isolation of Carnation Ringspot Virus by cDNA Hybridization Analysis and Serology

AN De-Rong, WEI Ning-Sheng, R.Koeni

1989, 4(4): 408

A virus isolate (w) has been obtained from the Oker river in W.Germany. The isolate was identified as Carnation ringspot virus (CarRSV) by means of inoculation on the test plant, electron microscopy, dot blot hybridization and serology.The molecular weight of the coat protein is 3.8×10~4. Northern blot analysis revealed that the two RNA species of the virus which consisted of c. 3.7 and 1.5kb, respectively, but without base sequence homology.

Effects of Temperature on ToMV Multiplication and Changes of Soluble Leaf Proteins in Tomato

XU Ren-Lin, YI Qiong-Hua

1989, 4(4): 415

ToMV concentrations in virus inoculated tomato plants were analyzed by local lesion test. The results showed that virus concentrations in resistan-t line GCR-267 was 1/40—1/50 that in susceptible line GCR-26. ToMV multiplication was strongly inhibited by gene Tm-2~a, which was not influenced by temperature. ToMV multiplication in GCR-26 was dependent on temperature, the higher the temperatature, the less the virus concentration. The changes of the soluble leaf proteins of tomato plants grown at constant or ...

The cDNA of Assembly Origin Sequence in TMV Genome Inhibiting TMV Assembly in Vitro

PENG Hai, SHEN Xue-Ren, WU Jian-Hua, GONG Zu-Xun

1989, 4(4): 427

A 20 nucleotides DNA fragment which is complementary with the assembly origin of TMV-RNA was synthesized. Instead of antisense RNA, the DNA fragment could hybridize with TMV-RNA in nitrocellulose dot hybridization and in mini-volume solution hybridization assays. Using RNA-cDNA complex and 20S aggregates of TMV coat protein, the TMV in vitro assembly reaction experiment, which was monitored by 310nm absorption spectrum and electron microscopy, showed that TMV in vitro assembly could not proceed in results o...

Detection of Pararotavirus ( Group C ) in Piglets

WANG Zheng-Dang, WANG Lin-Qing, ZHANG Yan, BANG Ke-Gao, WANG Jia-Fu, JIANG Shan-Tian, HONG Deng-Rong, ZHANG Wu-Bei

1989, 4(4): 433

20 diarrheal samples from piglets of a pig farm were examined with PAGE, and 11 of them manifested the pararotavirus (group C) RNA electrophoretic profile. An acute watery diarrhea occurred after inoculating newborn colostrumdeprived piglets.Rotavirus (group A) ELISA and pararotavirus (group B) counter immunoelectrophoresis showed that the virus has no serologic relations with group A and B rotaviruses.

Analysis of Influenza Viruses by Pyrolysis Gas Chromatography

MENG Lu-Ying, XIAO Jian, WANG Ya-Dong

1989, 4(4): 438

Five strains of influenza and one strain of newcastle disease virus were analysed by pyrolysis gas chromatography (PGC). The different patterns between the normal and the infected allantois fluid samples which included influenza viruses type A, B and newcastle disease virus were clearly shown in the pyrograms. But the differences among the influenza subtype A_1, A_2, and A_3 were not clear. Their pyrograms showed similar characters.From the above findings it was concluded that PGC can be used as a tool for ...
Research Article

Isolation of Enterovirus Type 71 from the Vesicle Fluid of an Adult Patient with Hand-footmouth Disease in China

ZHENG Zhi-Ming (郑志明), ZHANG Jiang-Hong (张江虹), CHU Wei-Ping (褚卫平), HE Pei-Jun (何佩君)

1989, 4(4): 375

Isolation and characterization of a H isolate of enterovirus type 71 (E71) from the vesicle fluid of hand-foot-mouth disease have been reported in present study.The H isolate can grow at primary human embryo lung cells, MA104 cells, and BSC cells and results in a typical enterovirus cytopathic effect. 1-day-old mice inoculated ip with H isolate showed limb paralysis and died at 6th day postinfection. Numerous mature virus particles with a diameter of 25 nm in crystal arrays in the cytoplasm have been visualized under microscope. The paired sera of the patient have been tested and showed a 4-fold increase of neutralizing antibody to H isolate. Using 100 antibody units of anti-Cox A5, 7, 9, 16, E70, E71, and 50 antibody units of enterovirus LBM typing pools A-H as well as McAb p27 of E71 BrCr strain, H isolate could be only neutralized by both anti-E71 polyclone and monoclone antibodies. The minimal effective antibody units to H isolate are required respectively 1.6 for anti-E71 polyclone antibody and 64 for McAb p27. Furthermore, high titers of anti-Cox A16 have also showed some cross reaction to H isolate shares a common antigen with Cox A16.