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1992 Vol.07(2)

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Review

Advances of Polymerase Chain Reaction Applied in Virology

PAN Chun-Gang, TIAN Bo

1992, 7(2): 113

Present Status of Research on Molecular Biology of hepatitis D Virus

TANG Hong, ZHAO Lian-San, CAO Zhong-Liang

1992, 7(2): 118

A Survey on the Natural Infection of Murine Cytomegalovirus in Laboratory Mouse Colonies in China

SHANG Jia-Ming, YUAN Yun-Hua, CHENG Hong

1992, 7(2): 124

Up to present, few was reported about the natural infection of murineeytomegalovirus(MCMV) in laboratory mice. Based on the experimental infection of MCMV, we made a survey on the natural infection of MCMV in 10 common used strains of mice It was found that of 334 samples of submaxllary gland homogenate, none were positive for viral isolation, whereas specific anti-MCMV antibodies were found in 10.9-87.50% of different strains of mice. There were tendencies of increase in antibody positive rates as the mice...

Effect of Astragalus Membranaceus on T Lymphocyte Mediated Immunity in Mice with Coxsackie B3 Viral Myocarditis

WU Wei-Zhong, YANG Ying-Zhen, JIN Pei-Ying

1992, 7(2): 129

Effect of Astragalus Membranaceus(AM) in lymphocyte subsets in an animal model of viral myocarditis were investigated by antigen specific cytotoxicity assay, The percentage of Thyl+cell in the peripheral blood and spleen was significantly higher in AM treated mice than in normal saline(NS) treated mice on 7th, 9th, 14th day(P<0.05) after Coxsackie B3 viral(CB 3V) inoculation, while it was statistically lower in myocardium in AM treated mice on 14th day after infection The percentage of L3T4+cell was mark...

Preliminary Report of Cell Culture of Hepatitis E Virus

YIN Shu-Rong, LIU Yu-Tang, TIAN Xin, BAO Zuo-Yi, JIANG Yu-Tu, ZHANG Xi-Tan

1992, 7(2): 134

Hepatitis E virus(HEV) was isolated in human embryomic lung cell inoculated with liver tissue suspension from an HEV-infected rhesus monkey, Slight cytopathic effect(CPE) appeared at the 27th day after 4 blind passages of viruses in cell culturs to at 17th day after 7 passages. Low amounts of 27—34nm virus-like particles(VLPs) in cell cultures were visualized by immunoelectronmicroseopy(IEM). 2 of 3 rhesus monkeys inoculated with 20ml of pool of cell cultures from 4 to 7 passages developed significant eleva...

A Method for Detection of Anti-HBc by ABC-ELISA and Its Epidemiological and Clinical Application

JIANG Ke-Qiang, PENG Qin-Lin, E Hai-Chun

1992, 7(2): 138

The method of using ABC-ELISA to measure anti-HBC in the serum of patients with hepatitis B was developed. The experimental results indicated that the sensitivity of ABC-ELISA was five times higher than that of the conventional ELISA. On the average, the positive detection rate of anti-HBc by means of ABC-ELISA increased by 42.42% over the one by using conventional ELISA. Repeated experiments showed good reproductivity. Alternative tests using various antigens and antibodies of HBV and blockage test using p...

Change of the Antibody Level among Inhabitants after the First Epidemic of AHC Caused by CA24v

TUN Jia-Ju, LIU Yan-Ming, ZHANG Qi-Liang, HU Bi-Yong, CHEN Mao-Yi

1992, 7(2): 144

Half and one year after the epidemic of acute hemorrhagic conjunctivitis(AHC) caused by eoxsackievirus A24 variant(CA24v) in the autumn of 1988, 423 sera were collected and the neutralization antibody(NA) and specific IgM against CA24v were tested in microplate with Hela cells. The positive rate of NA was 37.9% in the spring and 48.8% in the autumn of 1989. The specific IgM was 3.3% in the spring and 6.7% in the autumn of 1989 and one strain of CA24v was isolated from a cold-like patient at this time. The r...

Estimation of the Rates of Haemagglutinin Mutation of Influenza A Viruses Isolated in Recent years Using the 125 I-Iabeled Peptide Mapping

YAN Jia-Xin, HUANG He, BO Nan-Sheng, YAO Jie

1992, 7(2): 149

A sensitive and simplified method of ~(125)I-labeled peptide mapping was used to estimate the rates of the haemagglutinin(HA) mutation of 5 strains of influenza Al viruses and 5 strains of influenza A3 viruses. It could be calculated that the average rates of HA mutation for these two subtypes of viruses are 0.47% and 0.44% per year respectively. The strains isolated in 1986 showed some specialities as compared with the average rates of HA mutation The relation between the rates of HA mutation and the epide...

Further Studies on Serotyping and Antigenic Analysis of Hemorrhagic Fever with Rendal Syndrome Viruses by Plaque Reduction Neutralization Test

YU Yong-Xin, YAO Zhi-Hui, AN Qi

1992, 7(2): 156

Serotypic classification of 14 HFRS virus isolates from different sources were studied using plaque reduction tests(PRNT) sera of rabbits inoculated with different HFRS viruses were tested against reference type-1 Hantaan virus and type-2 Seoul virus. The results indicated that except one strain(K_(24)), all the 13 isolates could be classified into either serotype 1 or serotype 2 according to the differences(≥4 fold) of the neutralizing titers between the sera reacted against reference type 1 and type 2 vir...

Use of Digoxigenin Labelled RNA Probe in Hepatitis D Virus RNA Hybridization

NIU Jian-Zhang, GUO Ge-Jian, Edwards P., Bowden D.S.

1992, 7(2): 160

A RNA hyhridization assay for hepatitis D virus in serum and liver specimens, from human and infected chimpanzee experimentally, was described using a nonradioactive Digoxigenin labelled RNA probe, and was compared with the ~(32)P labelled RNA probe. Findings show that the sensitivity and specificity of the two probes are the same(about 0.2pg of homologous DNA). The results indicated that Digoxigenin labelled RNA probe can be used for efficacious detection of HDV RNA in serum and liver tissue from human or ...

Biotinylated Lectin Blotting Analysis of Dengue-2 Virus Glycoproteins

CHEN Sheng-Min, GUO Hui-Yu

1992, 7(2): 166

In this study we developed a method for characterization of the leetin binding properties of D2V by a panal of lectins with different sugar specificity. The dengue-2 virus(D2V) (New Guinea C strain) was cultured in Aedes alhopietus C6/36 cells. The virus-speeific and cellular proteins of the infected and mock-infected cell lysates were separated by diseontinous SDS-PAGE and transferred electrophoretically onto NC sheet. The blots were then probed with 12 kinds of biotinylated leetius(Con A, PSA, LCA, SBA, D...

Neutralizing Antibody Responses in Human Immunized with M. Gerbil Kidney Tissue Culture Inactivated HFRS Vaccine Assessed by Two Methods

SHU Yong-Xin, LIU Wen-Xue, NIE Zi-Lin

1992, 7(2): 176

In this study, expanded human trial with M, Gerbil kidney tissue culture inactivated HFRS vaccine was carried out and neutralizing antibody response was assessed by plaque reduction neutralization(PRNT) and CPE neutralization(CPENT) methods. According to the data of all 74 person immune sera assayed by the two methods, the rates of seroconversion and GMT tesed by CPENT were significantly higher than that by PRNT. Several vaccinating groups were studied and the neutralizing antibody levels were as follows: v...

Monoclonal Antibodies to Respiratory Syncytial Virus and Their Application in Identificating the Virus Protein Specificity and Comparing Their Antigenic Difference between Virus Isolates

TIAN Mu-Zhen, DONG Ji-Hua, TU Fu-Xun, XIE Gong-Bin, CHAI Rui-Zhen

1992, 7(2): 181

The BALB/C mice were immunized with HeLa cells infected with respiratory syncytial virus(RSV, Wuhan strain). The immunized spleen cells were fused with SP2/0 myeloma cells which were obtained from the SP2/0 solid tumor grown in BALB/C mice. Thirteen hybridoma cell lines secreting antiRSV monoclonal antibody(MeAb) were established. The chromosome number of these cell lines was ranged from 94—104 and the seerated MeAbs belonged to routine IgG1, IgG2a, IgG2b subclasses. The ascites antibody titer was from 1: 1...

Isolation and Some Properties of Periplaneta fuliginosa Virus

YANG Ming-Hui, CHEN Tao, LIU Yong, LIANG Shi-Ping, WANG Chun-Sheng, XIA Ke-Xiang, SUN Fu-Lin

1992, 7(2): 186

Recently, we isolated a non-occluded virus from spontaneous mortal Periplaneta fuliginosa(Serville). The virus suspension was mixed in sterile food to feed healthy P. fuliginosa nymphs(of 154—169 days). The percent mortality of the nymphs which died with infected virus was over 98%. Under electronmicroscope the virus was isometric particle about 23nm in diameter. The ultraviolet absorption of the virus was as character as nucleoprotein Purified virus suspension was used to distinguish the nucleic acid kinds...

Preliminary Study on Yellow Dwarf of Corn infected by BYDV

ZHANG Qin-Feng, ZHAO Yu-Xia, YANG Ying, JIN Xin-Zao, SHU Xiang-San

1992, 7(2): 192

It had been manifested that corn was readily infected by wheat BYDV The symptoms were similar to those of the wheat The most effective veetor aphid species was R. padi, while M. avena, S. graminnm and R. maidis were also among the vectors. The isolates belongs to the main strain of BYDV, eg. S. graminum/R. padi(GPV) strain. Mutual transmission in the fields between wheat and corn was evident.

Diagnosis of Virus on Gadic Virus Disease of Zhejiang

LU Guan-Cheng, HONG Jian, XU Zheng, HU Shi-Meng

1992, 7(2): 197

In Zhejiang, Jiangsu and other provinces, 5 varieties of the garlic crops are totally diseased. The virus disease frequently causes degeneration of viruliferousness, loss of yields and reduction of tolerant to store. Symptoms due to the infection of virus are chlorotic mottling between the veins, yellow streaking and mosaic, scarcely visible and leaf distortion or deformation. The virus can be artificially inoculated on Vicia faba, then produces brown local lesions, slight spot on Chenopodium murale, necrot...

Localization of Barley Mild Mosaic Virus in Infected Barley Cells by Immuno-Colloidal Gold Labelling

CHEN Jian-Beng, M J Adams

1992, 7(2): 207

An immuno-colloidal gold labelling procedure was developed to localize barley mild mosaic virus(BaMMV) in thin sections of barley leaf and root tissues embedded in L. R. White resin, The optimal procedure for specific labelling of virus with a minimum of background labelling and economical use of reagents was to treat sections with buffer containing bovine serum albumen to block non-specific binding sites, treat with BaMMV-IgG(1/1000; 15 rain at room temperature), wash and label with goat anti-rabbit IgG-go...

Studies on Strains of Cucumber Mosaic Virus on Tomato

LIU Huan-Ting, SHU Han-Cheng, YAN Dun-Yu, GUO Xin-Qi

1992, 7(2): 216

During 1986—1989, 111 isolates of cucumber mosaic virus were obtained from tomato fields in Shandong province According to the host ranges and symptoms on different hosts, three pathogen strains of CMV were established as tomato fern leaf strain(CMV-ToF), tomato mosaic strain(CMV-ToM) and tomato latent(light mosaic) strain(CMV-ToL). There were some difference not only in bio-charaeteristics, but also in electrophoretic mobility and morphology of purified virus particle, molecular weight of capsid polypeptid...

Three Isolates of Retrovirus from Chinese Rhesus Monkeys

ZHANG Xin-Sheng, NI Yan-Ping, WU Chi-Ping, XU Jian, CHEN De-Hui, SHU Zi-Zheng, Nicholas.W.Lercbe

1992, 7(2): 224

Three strains of retrovius, which can give positive results both by ELISA and immunofluoreseenee assay(IFA) with SRV-1 newly have been isolated from Rhesus monkeys(Macaca mulatta) catehed frim three areas of China. It have been confirmed by Western Blot that their seroantibodies response with SRV-1 positively. One of them occurs cross-response with of SRV-1 and STLV. Lots of the virus particles having typical ultrastuctures of retrovirus have been observed by electron microscope.

The Study of Methodology for Testing the Survival of Viral Aerosol

TONG Yong-Yi, HUANG Zi-Cai, FU Xiao-Jun, CHEN Zhen-Sheng, ZHANG Chao-Long, CHE Feng-Xiang

1992, 7(2): 229

With serotype B271 of Corynebacterium crenatum phage as a model, we established a method for studying the survival of aerosol of fine biological particles such as virus. The paper discussed some important techniques for the study of survival of viral aerosols, including the viral resistance to aerosolization, the size distribution of the particles of viral aerosols, caculation of physical decay with aerosol tracer and recovery technique for viral aerosol. It provided a general guide for the study of the sur...

Derivation of the Formulas for Triangulation Number and Capsomer Number

WANG Ji-Ke

1992, 7(2): 236

This paper reports the derivation and calculation of several formulas for triangulation number and eapsomer number, The formulas are P=h~2+hk+k~2, T=pf~2, C=10T+2 and C=10p(n-1)~2+2. And the correlations among the formulas and the meaning of the parameters are given.