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Citation: Ying Tang, Yiqin Wang, Yuchang Li, Huai Zhao, Sen Zhang, Ying Zhang, Jing Li, Yuehong Chen, Xiaoyan Wu, Chengfeng Qin, Tao Jiang, Xiaoping Kang. An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2 [J].VIROLOGICA SINICA, 2022, 37(1) : 138-141.  http://dx.doi.org/10.1016/j.virs.2022.01.006

An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2

  • Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a novel coronavirus that causes the outbreak of coronavirus disease 2019 (COVID-19) (Li et al., 2020a). Viral nucleic acid testing is the standard method for the laboratory diagnosis of COVID-19 (Wu et al., 2020a; Zhu et al., 2020). Currently, a variety of qPCR-based detection kits are used for laboratory-based detection and confirmation of SARS-CoV-2 infection (Corman et al., 2020; Hussein et al., 2020; Ruhan et al., 2020; Veyer et al., 2020). Conventional qPCR involves virus inactivation, nucleic acid extraction, and qPCR amplification procedures. Therefore, the process is complicated, which usually takes longer than 2 h, and requires biosafety laboratories and professional staff. Thus, qPCR is not suitable for use in field or medical units. To reduce the operation steps, automatic integrated qPCR detection systems that combine nucleic acid extraction and qPCR amplification in a sealed cartridge were developed to detect viruses in clinical samples (Li et al., 2020b). However, the detection time is still longer than 1 h. Therefore, rapid nucleic acid detection systems are needed to further improve the detection efficiency.

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  • 10.1016j.virs.2022.01.006-ESM.docx
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    2. Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, Bleicker T, Brunink S, Schneider J, Schmidt ML, Mulders DG, Haagmans BL, van der Veer B, van den Brink S, Wijsman L, Goderski G, Romette JL, Ellis J, Zambon M, Peiris M, Goossens H, Reusken C, Koopmans MP, Drosten C (2020) Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill 25:2000045.

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    6. Li Y, Li J, Zhang Y, Dai L, Li L, Liu J, Zhang S, Wu X, Hu Y, Qin C, Jiang T, Kang X (2020b) Development of an automatic integrated gene detection system for novel severe acute respiratory syndrome-related coronavirus (SARS-CoV2). Emerg Microbes Infect 9:1489-1496.

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    10. Wang J, Liu L, Wang J, Sun X, Yuan W (2017) Recombinase polymerase amplification assay-A simple, fast and cost-effective alternative to real time PCR for specific detection of feline herpesvirus-1. PLoS One 12:e0166903.

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    An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2

      Corresponding author: Tao Jiang, jiangtao@bmi.ac.cn
      Corresponding author: Xiaoping Kang, kangxiaoping@163.com
    • a Department of Virology, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing 100071, China
    • b State Key Laboratory of Pathogen and Biosecurity, AMMS, Beijing 100071, China
    • c Beijing University of Chemical Technology, Beijing 100029, China
    • d Technology Center of Erenhot Customs, Erenhot 011100, China
    • e Lifereal Biotech. Inc., Hangzhou 311100, China
    • f Anhui Medical University, Hefei 230032, China

    Abstract: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a novel coronavirus that causes the outbreak of coronavirus disease 2019 (COVID-19) (Li et al., 2020a). Viral nucleic acid testing is the standard method for the laboratory diagnosis of COVID-19 (Wu et al., 2020a; Zhu et al., 2020). Currently, a variety of qPCR-based detection kits are used for laboratory-based detection and confirmation of SARS-CoV-2 infection (Corman et al., 2020; Hussein et al., 2020; Ruhan et al., 2020; Veyer et al., 2020). Conventional qPCR involves virus inactivation, nucleic acid extraction, and qPCR amplification procedures. Therefore, the process is complicated, which usually takes longer than 2 h, and requires biosafety laboratories and professional staff. Thus, qPCR is not suitable for use in field or medical units. To reduce the operation steps, automatic integrated qPCR detection systems that combine nucleic acid extraction and qPCR amplification in a sealed cartridge were developed to detect viruses in clinical samples (Li et al., 2020b). However, the detection time is still longer than 1 h. Therefore, rapid nucleic acid detection systems are needed to further improve the detection efficiency.

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