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Citation: Lifang Song, Bopei Cui, Jinghuan Yang, Xiaotian Hao, Xujia Yan, Jialu Zhang, Dong Liu, Ziyang Song, Qian Wang, Qunying Mao, Zhenglun Liang. Construction and verification of an infectious cDNA clone of coxsackievirus B5 [J].VIROLOGICA SINICA, 2022, 37(3) : 469-471.  http://dx.doi.org/10.1016/j.virs.2022.03.005

Construction and verification of an infectious cDNA clone of coxsackievirus B5

  • Corresponding author: Qunying Mao, maoqunying@126.com
    Zhenglun Liang, lzhenglun@126.com
  • Received Date: 07 September 2021
    Accepted Date: 07 March 2022
    Available online: 11 March 2022
  • Highlights:
    · An infectious cDNA clone of CV-B5 was constructed.
    · The rescued and parental virus possessed similar biological characteristics.
    · The virulence of the rescued virus was similiar to that of the parental virus.
    · Viral distribution and tissue tropism of those two viruses were in agreement.

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  • 10.1016j.virs.2022.03.005-ESM.docx
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    7. Hao, X., Song, S., Zhong, Q., Jamal-U-Ddin, H., Guo, J., Wu, Y., 2021. Rescue of an infection clone of barley yellow dwarf virus -GAV. Phytopathology 111, 2383-2391.

    8. Li, P., Yao, C., Wang, T., Wu, T., Yi, W., Zheng, Y., Miao, Y., Sun, J., Tan, Z., Liu, Y., Zhang, X., Wang, H., Zheng, Z., 2021. Recovery of a far-eastern strain of tick-borne encephalitis virus with a full-length infectious cDNA clone. Virol. Sin. 36, 1375-1386.

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    11. Mao, Q., Hao, X., Hu, Y., Du, R., Lang, S., Bian, L., Gao, F., Yang, C., Cui, B., Zhu, F., Shen, L., Liang, Z., 2018. A neonatal mouse model of central nervous system infections caused by Coxsackievirus B5. Emerg. Microb. Infect. 7, 185.

    12. Pan, M., Gao, S., Zhou, Z., Zhang, K., Liu, S., Wang, Z., Wang, T., 2018. A reverse genetics system for enterovirus D68 using human RNA polymerase I. Virus Gene. 54, 484-492.

    13. Sciandra, I., Falasca, F., Maida, P., 2020. Seroprevalence of group B coxsackieviruses:retrospective study in an Italian population. J. Med. Virol. 92, 3138-3143.

    14. Wang, M., Yan, J., Zhu, L., Wang, M., Liu, L., Yu, R., Chen, M., Xun, J., Zhang, Y., Yi, Z., Zhang, S., 2020. The establishment of infectious clone and single round infectious particles for coxsackievirus A10. Virol. Sin. 35, 426-435.

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    16. Zaoutis, T., Klein, J., 1998. Enterovirus infections. Pediatr. Rev. 19, 183-191.

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    Construction and verification of an infectious cDNA clone of coxsackievirus B5

      Corresponding author: Qunying Mao, maoqunying@126.com
      Corresponding author: Zhenglun Liang, lzhenglun@126.com
    • a National Institute for Food and Drug Control, Beijing 102629, China;

    Abstract: Highlights:
    · An infectious cDNA clone of CV-B5 was constructed.
    · The rescued and parental virus possessed similar biological characteristics.
    · The virulence of the rescued virus was similiar to that of the parental virus.
    · Viral distribution and tissue tropism of those two viruses were in agreement.

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