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Citation: Yuanzhi Chen, Chenguang Shen, Jing Chen, Junyu Chen, Fentian Chen, Limin Zhang, Xue Liu, Siyuan Chen, Sen Xue, Yongliang Liu, Jixian Tang, Quan Yuan, Yixin Chen, Wenxin Luo, Ningshao Xia. Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells [J].VIROLOGICA SINICA, 2022, 37(4) : 619-622.  http://dx.doi.org/10.1016/j.virs.2022.03.009

Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

  • Highlights
    1. Class-switch recombination was mimicked in hybridomas through a controllable expression system of activation-induced cytidine deaminase.
    2. IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1.
    3. IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo.

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  • 10.1016j.virs.2022.03.009-ESM.docx
    1. Caini, S., Huang, Q.S., Ciblak, M.A., Kusznierz, G., Owen, R., Wangchuk, S., Henriques, C.M., Njouom, R., Fasce, R.A., Yu, H., Feng, L., Zambon, M., Clara, A.W., Kosasih, H., Puzelli, S., Kadjo, H.A., Emukule, G., Heraud, J.M., Ang, L.W., Venter, M., Mironenko, A., Brammer,L., Maile, T.Q., Schellevis,F., Plotkin,S., Paget,J., Global Influenza,B.S., 2015. Epidemiological and virological characteristics of influenza b: results of the global influenza b study. Influenza Other Respir. Viruses 9 (Suppl. 1), 3–12.

    2. Corti, D., Lanzavecchia, A., 2013. Broadly neutralizing antiviral antibodies. Annu. Rev. Immunol. 31, 705–742.

    3. Iglesias-Ussel, M.D., Fan, M., Li, Z., Martin, A., Scharff, M.D., 2006. Forced expression of aid facilitates the isolation of class switch variants from hybridoma cells. J. Immunol. Methods 316, 59–66.

    4. Ku, Z., Xie, X., Hinton, P.R., Liu, X., Ye, X., Muruato, A.E., Ng, D.C., Biswas, S., Zou, J., Liu, Y., Pandya, D., Menachery, V.D., Rahman, S., Cao, Y.A., Deng, H., Xiong, W., Carlin, K.B., Liu, J., Su, H., Haanes, E.J., Keyt, B.A., Zhang, N., Carroll, S.F., Shi, P.Y., An, Z., 2021. Nasal delivery of an igm offers broad protection from sars-cov-2 variants. Nature 595, 718–723.

    5. Pan, Y., Zhang, Y., Yang, P., Qian, H., Shi, W., Wu, S., Cui, S., Zhang, D., Wang, Q., 2015. Epidemiological and phylogenetic characteristics of influenza b infection in severe acute respiratory infection cases in beijing, 2014 to 2015. Medicine (Baltim.) 94, e2399.

    6. Shen, C., Zhang, M., Chen, Y., Zhang, L., Wang, G., Chen, J., Chen, S., Li, Z., Wei, F., Chen, J., Yang, K., Guo, S., Wang, Y., Zheng, Q., Yu, H., Luo, W., Zhang, J., Chen, H., Chen, Y., Xia, N., 2019. An igm antibody targeting the receptor binding site of influenza b blocks viral infection with great breadth and potency. Theranostics 9, 210–231.

    7. Stavnezer, J., Guikema, J.E., Schrader, C.E., 2008. Mechanism and regulation of class switch recombination. Annu. Rev. Immunol. 26, 261–292.

    8. Su, Y.C., Al-Qaisi, T.S., Tung, H.Y., Cheng, T.L., Chuang, K.H., Chen, B.M., Roffler, S.R., 2014. Mimicking the germinal center reaction in hybridoma cells to isolate temperature-selective anti-peg antibodies. mAbs 6, 1069–1083.

    9. Subbarao, K., Matsuoka, Y., 2013. The prospects and challenges of universal vaccines for influenza. Trends Microbiol 21, 350–358.

    10. van de Sandt, C.E., Bodewes, R., Rimmelzwaan, G.F., de Vries, R.D., 2015. Influenza b viruses: not to be discounted. Future Microbiol 10, 1447–1465.

    11. Walker, L.M., Burton, D.R., 2018. Passive immunotherapy of viral infections: ‘Superantibodies’ enter the fray. Nat. Rev. Immunol. 18, 297–308.

    12. Wilson, P.C., Andrews, S.F., 2012. Tools to therapeutically harness the human antibody response. Nat. Rev. Immunol. 12, 709–719.

    13. Yerabham, A., Ho, M., 2021. A novel igm intranasal intervention against sars-cov-2. Antib. Ther. 4, 171–174.

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    Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

      Corresponding author: Chenguang Shen, a124965468@smu.edu.cn
      Corresponding author: Yixin Chen, yxchen2008@xmu.edu.cn
      Corresponding author: Wenxin Luo, wxluo@xmu.edu.cn
    • a National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health & School of Life Science, Xiamen University, Xiamen 361102, China;

    Abstract: Highlights
    1. Class-switch recombination was mimicked in hybridomas through a controllable expression system of activation-induced cytidine deaminase.
    2. IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1.
    3. IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo.

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