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Citation: Mengyuan Chen, Shuizhen He, Hualong Xiong, Dongxu Zhang, Shaojuan Wang, Wangheng Hou, Xiaomei Zhu, Jin Wang, Yang Huang, Congming Hong, Yubin Wu, Ruoyao Qi, Tianying Zhang, Quan Yuan, Tingdong Li, Yixin Chen, Shiyin Zhang, Shengxiang Ge, Jun Zhang, Ningshao Xia. New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq [J].VIROLOGICA SINICA, 2022, 37(5) : 758-761.  http://dx.doi.org/10.1016/j.virs.2022.07.001

New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

  • Highlights
    1 A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq.
    2 Spep-1 showed nanomolar affinity and high specificity to spike protein.
    3 S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples.
    4 Spep-1 can be used in future on S2 recognition, virus tracing and drug delivery.

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  • 10.1016j.virs.2022.07.001-ESM.docx
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    2. Gavor, E., Choong, Y., Er, S., Sivaraman, H., Sivaraman, J., 2020. Structural basis of SARSCoV-2 and SARS-CoV antibody interactions. Trends Immunol. 41, 1006-1022.

    3. Hao, J., Wang, F., Xing, G., Liu, Y., Deng, R., Zhang, H., Cheng, A., Zhang, G., 2019. Design and preliminary application of affinity peptide based on the structure of the porcine circovirus type II capsid (PCV2 cap). PeerJ 7, e8132.

    4. Harvey, W., Carabelli, A., Jackson, B., Gupta, R., Thomson, E., Harrison, E., Ludden, C., Reeve, R., Rambaut, R.,, COVID-19 Genomics UK (COG-UK) Consortium, Peacock, S., Robertson, D., 2021. SARS-CoV-2 variants, spike mutations and immune escape. Nat. Rev. Microbiol. 19, 409-424.

    5. Mendoza-Figueroa, J., Kvarnheden, A., Méndez-Lozano, J., Rodríguez-Negrete, E., Monteros, R., Soriano-García, M., 2018. A peptide derived from enzymatic digestion of globulins from amaranth shows strong affinity binding to the replication origin of tomato yellow leaf curl virus reducing viral replication in nicotiana benthamiana. Pestic. Biochem. Physiol. 145, 56-65.

    6. Ng, K., Mohd-Ismail, N., Tan, Y., 2021. Spike S2 subunit:the dark horse in the race for prophylactic and therapeutic interventions against SARS-CoV-2. Vaccines (Basel) 9, 178.

    7. Norman, A., Franck, C., Christie, M., Hawkins, H., Patel, K., Ashhurst, A., Aggarwal, A., Low, J., Siddiquee, R., Ashley, C., Steain, M., Triccas, J., Turville, S., Mackay, J., Passioura, T., Payne, R., 2021. Discovery of cyclic peptide ligands to the SARS-CoV-2 spike protein using mrna display. ACS Cent. Sci. 7, 1001-1008.

    8. Pei, P., Qin, H., Chen, J., Wang, F., He, C., He, S., Hong, B., Liu, K., Qiao, R., Fan, H., Tong, Y., Chen, L., Luo, S., 2021. Computational design of ultrashort peptide inhibitors of the receptor-binding domain of the SARS-CoV-2 S protein. Briefings Bioinf. 22, bbab243.

    9. Pomplun, S., Jbara, M., Quartararo, A., Zhang, G., Brown, J., Lee, Y., Ye, X., Hanna, S., Pentelute, B., 2021. De novo discovery of high-affinity peptide binders for the SARSCoV-2 spike protein. ACS Cent. Sci. 7, 156-163.

    10. Rosestedt, M., Velikyan, I., Rosenström, U., Estrada, S., Åberg, O., Weis, J., Westerlund, C., Ingvast, S., Korsgren, O., Nordeman, P., Eriksson, O., 2021. Radiolabelling and positron emission tomography imaging of a high-affinity peptide binder to collagen type 1. Nucl. Med. Biol. 93, 54-62.

    11. Singhal, S., Khanna, P., Khanna, L., 2021. Synthesis, comparative in vitro antibacterial, antioxidant and UV fluorescence studies of bis indole schiff bases and molecular docking with ct-DNA and SARS-CoV-2 Mpro. Luminescence 36, 1531-1543.

    12. Ucar, B., Acar, T., Arayici, P., Derman, S., 2021. A nanotechnological approach in the current therapy of COVID-19:model drug oseltamivir-phosphate loaded PLGA nanoparticles targeted with spike protein binder peptide of SARS-CoV-2.Nanotechnology. https://doi.org/10.1088/1361-6528/ac1c22.

    13. Xiong, H., Wu, Y., Cao, J., Yang, R., Liu, Y., Ma, J., Qiao, X., Yao, X., Zhang, B., Zhang, Y., Hou, W., Shi, Y., Xu, J., Zhang, L., Wang, S., Fu, B., Yang, T., Ge, S., Zhang, J., Yuan, Q., Huang, B., Li, Z., Zhang, T., Xia, N., 2020. Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells. Emerg. Microb. Infect. 9, 2105-2113.

    14. Zhao, H., To, K., Sze, K., Yung, T., Bian, M., Lam, H., Yeung, M., Li, C., Chu, H., Yuen, K., 2020. A broad-spectrum virus- and host-targeting peptide against respiratory viruses including influenza virus and SARS-CoV-2. Nat. Commun. 11, 4252.

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    New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq

      Corresponding author: Shiyin Zhang, zhangshiyin@xmu.edu.cn
      Corresponding author: Shengxiang Ge, sxge@xmu.edu.cn
    • a State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen University, South Xiang'an Road, Xiamen, 361102, China;
    • b Xiamen Haicang Hospital, Haiyu Road, Xiamen, 361026, China;
    • c School of Life Sciences, Xiamen University, South Xiang'an Road, Xiamen, 361102, China

    Abstract: Highlights
    1 A peptide Spep-1 targeting S2 of SARS-CoV-2 spike protein was selected by PhIP-Seq.
    2 Spep-1 showed nanomolar affinity and high specificity to spike protein.
    3 S-1 based immunoassay can detect femtomolar spike antigen in spiked serum samples.
    4 Spep-1 can be used in future on S2 recognition, virus tracing and drug delivery.

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