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Citation: Kaw Bing Chua, Qimei Ng, Tao Meng, Qiang Jia. Development of stable, cold-adapted, temperature-sensitive/conditional lethal chimeric enterovirus A71 and coxsackievirus A16 [J].VIROLOGICA SINICA, 2022, 37(5) : 769-773.  http://dx.doi.org/10.1016/j.virs.2022.08.001

Development of stable, cold-adapted, temperature-sensitive/conditional lethal chimeric enterovirus A71 and coxsackievirus A16

  • Corresponding author: Kaw Bing Chua, chuakb@tll.org.sg
  • Received Date: 17 August 2021
    Accepted Date: 02 August 2022
    Available online: 11 August 2022
  • Highlights
    1 A stable EV-A71 virus vector was created to generate chimeric enterovirus strains expressing capsid protein genes of EV-A71 subgenogroup C5 and CA16.
    2 Phenotypic and genetic stability of the generated chimeric EV-A71 and CA16 were analyzed.
    3 The amino acids at the cleavage site between VP1 and 2A is crucial for stability.

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  • 10.1016j.virs.2022.08.001-ESM.docx
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    7. Haller, A.A., Semler, B.L., 2005. Translation and host cell shutoff. In:Rotbart, H.A.(Ed.), Human Enterovirus Infections. ASM Press, Washington DC, pp. 113-133(Chapter 5).

    8. Hayward, J.C., Gillespie, S.M., Kaplan, K.M., Packer, R., Pallansch, M., Plotkin, S., Schonberger, L.B., 1989. Outbreak of poliomyelitis-like paralysis associated with enterovirus 71. Pediatr. Infect. Dis. 8, 611-616.

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    12. Liu, C.C., Tseng, H.W., Wang, S.M., Wang, J.R., Su, I.J., 2000. An outbreak of enteovirus 71 infection in Taiwan, 1998:epidemiologic and clinical manifestations. J. Clin. Virol. 17, 23-30.

    13. Liu, Y., Wang, C., Mueller, S., Paul, A.V., Wimmer, E., Jiang, P., 2010. Direct interaction between two viral proteins, the nonstructural protein 2CATPase and the capsid protein VP3, is required for enterovirus morphogenesis. PLoS Pathog. 6, e1001066, 1-14.

    14. Lum, L.C.S., Wong, K.T., Lam, S.K., Chua, K.B., Goh, A.Y.T., Lim, W.L., Ong, B.B., Paul, G., AbuBakar, S., Lambert, M., 1998. Fatal enterovirus 71 encephalitis. J. Pediatr. 133, 795-798.

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    16. Melnick, J.L., 1996. Enteroviruses:polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In:Fields, B.N., Knipe, D.M., Howley, P.M., et al. (Eds.), Fields Virology, third ed. Lippincott-Raven, Philadelphia, pp. 655-712.

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    Development of stable, cold-adapted, temperature-sensitive/conditional lethal chimeric enterovirus A71 and coxsackievirus A16

      Corresponding author: Kaw Bing Chua, chuakb@tll.org.sg
    • Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604, Singapore

    Abstract: Highlights
    1 A stable EV-A71 virus vector was created to generate chimeric enterovirus strains expressing capsid protein genes of EV-A71 subgenogroup C5 and CA16.
    2 Phenotypic and genetic stability of the generated chimeric EV-A71 and CA16 were analyzed.
    3 The amino acids at the cleavage site between VP1 and 2A is crucial for stability.

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