Ungulate bocaparvovirus 4 and rodent bocavirus are different genotypes of the same species of virus

Wenqiao He; Yuhan Gao; Yuqi Wen; Xuemei Ke; Zejin Ou; Jiaqi Fu; Mingji Cheng; Yun Mo; Qing Chen

doi:10.1016/j.virs.2022.02.002

April 2022

Wenqiao He, Yuhan Gao, Yuqi Wen, Xuemei Ke, Zejin Ou, Jiaqi Fu, Mingji Cheng, Yun Mo and Qing Chen. Ungulate bocaparvovirus 4 and rodent bocavirus are different genotypes of the same species of virus[J]. Virologica Sinica, 2022, 37(2): 215-222. doi: 10.1016/j.virs.2022.02.002

Citation: Wenqiao He, Yuhan Gao, Yuqi Wen, Xuemei Ke, Zejin Ou, Jiaqi Fu, Mingji Cheng, Yun Mo, Qing Chen. Ungulate bocaparvovirus 4 and rodent bocavirus are different genotypes of the same species of virus .VIROLOGICA SINICA, 2022, 37(2) : 215-222. http://dx.doi.org/10.1016/j.virs.2022.02.002

Ungulate bocaparvovirus 4和Rodent bocavirus是同一种病毒的不同基因型

何文巧 ^a ,
高钰涵 ^a ,
文羽祺 ^a ,
柯雪梅 ^b ,
欧泽金 ^a ,
傅嘉琪 ^a ,
程明基 ^a ,
莫云 ^a ,
陈清 ^a,,

a 南方医科大学公共卫生学院流行病学系
b 厦门市疾病预防控制中心

通讯作者： 陈清, 18002270308@163.com
收稿日期： 2021-04-07
录用日期： 2022-02-10

摘要

博卡病毒与许多人类传染病有关，例如呼吸道感染、肠胃炎和肝炎。已知鼠类是博卡病毒的宿主，可以携带啮齿动物博卡病毒和鼠博卡病毒。最近在鼠类中也检出了ungulate bocaparvovirus 4（一种猪博卡病毒）。研究鼠类携带的博卡病毒对于确定病毒来源和疾病的预防和控制非常重要。据我们所知，至今还没有研究对鼠类肝脏样本进行博卡病毒的调查。我们于2014年至2017年期间在中国南方地区捕获了624只鼠类。使用PCR方法对鼠类肝脏和血清样本中的博卡病毒进行了检测。在肝脏和血清样本中均检测到与ungulate bocaparvovirus 4和rodent bocavirus相关的序列。有趣的是，ungulate bocaparvovirus 4（参考毒株：KJ622366.1）在肝脏（分别为2.24%和0.64%）和血清样品（分别为2.19%和0.44%）中的检出率均高于rodent bocavirus（参考毒株：KY927868.1）。ungulate bocaparvovirus 4和rodent bocavirus相关序列的NS1区域在核酸和氨基酸水平上分别有超过84%和88%的一致性。此外，这些序列具有相似的基因组结构、基因组特征和密码子使用偏好，并可能具有共同的祖先。我们的研究结果表明，ungulate bocaparvovirus 4和rodent bocavirus可能来源于大鼠，并且可能是同一博卡病毒种的不同基因型。
- Ungulate bocaparvovirus 4
- , rodent bocavirus
- , 基因
- , 鼠

Ungulate bocaparvovirus 4 and rodent bocavirus are different genotypes of the same species of virus

Wenqiao He ^a ,
Yuhan Gao ^a ,
Yuqi Wen ^a ,
Xuemei Ke ^b ,
Zejin Ou ^a ,
Jiaqi Fu ^a ,
Mingji Cheng ^a ,
Yun Mo ^a ,
Qing Chen ^a,,

a Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, Guangzhou, 510515, China
b Xiamen Center for Disease Control and Prevention, Xiamen, 361000, China

Corresponding author: Qing Chen, 18002270308@163.com
Received Date: 07 April 2021
Accepted Date: 10 February 2022

Abstract

Bocaviruses are associated with many human infectious diseases, such as respiratory tract infections, gastroenteritis, and hepatitis. Rats are known to be reservoirs of bocaviruses, including rodent bocavirus and rat bocavirus. Recently, ungulate bocaparvovirus 4, a known porcine bocavirus, has also been found in rats. Thus, investigating bocaviruses in rats is important for determining the origin of the viruses and preventing and controlling their transmission. To the best of our knowledge, no study to date has investigated bocaviruses in the livers of rats. In this report, a total of 624 rats were trapped in southern China between 2014 and 2017. Liver and serum samples from rats were tested for the prevalence of bocaviruses using PCR. Sequences related to ungulate bocaparvovirus 4 and rodent bocavirus were detected in both liver and serum samples. Interestingly, the prevalence of ungulate bocaparvovirus 4 (reference strain:KJ622366.1) was higher than that of rodent bocavirus (reference strain:KY927868.1) in both liver (2.24% and 0.64%, respectively) and serum samples (2.19% and 0.44%, respectively). The NS1 regions of ungulate bocaparvovirus 4 and rodent bocavirus related sequences displayed over 84% and 88% identity at the nucleic acid and amino acid levels, respectively. Furthermore, these sequences had similar genomic structure, genomic features, and codon usage bias, and shared a common ancestor. These viruses also displayed greater adaptability to rats than pigs. Our results suggested that ungulate bocaparvovirus 4 and rodent bocavirus may originate from rats and may be different genotypes of the same bocavirus species.
- Ungulate bocaparvovirus 4
- , Rodent bocavirus (RoBoV)
- , Genome
- , Rats

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