Genotype distribution and evolutionary analysis of rotavirus associated with acute diarrhea outpatients in Hubei, China, 2013–2016

Ting Zhang; Jing Li; Yong-Zhong Jiang; Jun-Qiang Xu; Xu-Hua Guan; Li-Qiang Wang; Jie Chen; Yi Liang

doi:10.1016/j.virs.2022.05.005

August 2022

Ting Zhang, Jing Li, Yong-Zhong Jiang, Jun-Qiang Xu, Xu-Hua Guan, Li-Qiang Wang, Jie Chen and Yi Liang. Genotype distribution and evolutionary analysis of rotavirus associated with acute diarrhea outpatients in Hubei, China, 2013–2016[J]. Virologica Sinica, 2022, 37(4): 503-512. doi: 10.1016/j.virs.2022.05.005

Citation: Ting Zhang, Jing Li, Yong-Zhong Jiang, Jun-Qiang Xu, Xu-Hua Guan, Li-Qiang Wang, Jie Chen, Yi Liang. Genotype distribution and evolutionary analysis of rotavirus associated with acute diarrhea outpatients in Hubei, China, 2013–2016 .VIROLOGICA SINICA, 2022, 37(4) : 503-512. http://dx.doi.org/10.1016/j.virs.2022.05.005

2013-2016年湖北省急性腹泻门诊患者轮状病毒基因型分布及进化分析

张婷 ^a ,
李静 ^b ,
江永忠 ^b ,
徐军强 ^b ,
官旭华 ^b ,
王利强 ^a ,
陈杰 ^a ,
梁毅 ^a,,

武汉大学生命科学学院

通讯作者： 梁毅, liangyi@whu.edu.cn
收稿日期： 2021-03-26
录用日期： 2022-05-17

摘要

A组人类轮状病毒（RVA）每年在全球造成215，000名婴幼儿死亡，对人类健康构成严重威胁。我们对中国湖北省RVA的流行状况进行了哨点监测，以便掌握RVA的流行病学特征及遗传进化。2013年至2016年间，我们采集了湖北省4个地市轮状病毒监测网中的2007份急性胃肠炎门诊患者粪便样本，并通过实时RT-PCR对轮状病毒进行鉴定。我们分析了湖北省轮状病毒季节、年龄、性别特征和基因型分布。湖北省RVA感染主要发生在秋冬季。2007份样本中，RVA检测阳性率为7.62%。轮状病毒感染的重点人群为1～2岁门诊患者，检测阳性率最高，为16.97%。女性和男性间RVAs的检测阳性率没有显著差异。我们根据RVA的部分VP7/VP4基因序列对G/P基因型进行了系统发育分析。总共148个（96.7%）轮状病毒阳性标本被鉴定出G和P基因型：我们检测到5种G型、3种P型和7种G-P组合；其中G9基因型占整体G基因型的67%；G2、G1、G3、G4和未鉴定的G基因型合计占整体的33%。P[8]基因型占整体P基因型的78%；P[4]、P[6]和未鉴定的P基因型合计占22%。RVA最常见的基因型组合是G9P[8]（66%）。我们重构了湖北省RVA的进化时间尺度，发现RVA毒株的所有G9基因型具有不同的进化时间节点，2013-2016年间，同一基因型的RVA毒株有不同的进化来源。了解湖北省婴幼儿RVA的分布将有助于了解中国RVA的流行病学特征和遗传进化。
- A组人类轮状病毒
- , 流行病学
- , 系统发育分析
- , 进化
- , 湖北省

Genotype distribution and evolutionary analysis of rotavirus associated with acute diarrhea outpatients in Hubei, China, 2013–2016

a Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, 430072, China;

Corresponding author: Yi Liang, liangyi@whu.edu.cn
Received Date: 26 March 2021
Accepted Date: 17 May 2022

Abstract

Group A human rotaviruses (RVAs) annually cause the deaths of 215,000 infants and young children. To understand the epidemiological characteristics and genetic evolution of RVAs, we performed sentinel surveillance on RVA prevalence in a rotavirus-surveillance network in Hubei, China. From 2013 to 2016, a total of 2007 fecal samples from hospital outpatients with acute gastroenteritis were collected from four cities of Hubei Province. Of the 2007 samples, 153 (7.62%) were identified positive for RVA by real-time RT-PCR. RVA infection in Hubei mainly occurred in autumn and winter. The highest detection rate of RVA infection was in 1–2 years old of outpatients (16.97%). No significant difference of RVA positive rate was observed between females and males. We performed a phylogenetic analysis of the G/P genotypes based on the partial VP7/VP4 gene sequences of RVAs. G9P[8] was the most predominant strain in all four years but the prevalence of G2P[4] genotype increased rapidly since 2014. We reconstructed the evolutionary time scale of RVAs in Hubei, and found that the evolutionary rates of the G9, G2, P[8], and P[4] genotypes of RVA were 1.069×10^-3, 1.029×10^-3, 1.283×10^-3 and 1.172×10^-3 nucleotide substitutions/site/year, respectively. Importantly, using a molecular clock model, we showed that most G9, G2, P[8], and P[4] genotype strains dated from the recent ancestor in 2005, 2005, 1993, and 2013, respectively. The finding of the distribution of RVAs in infants and young children in Hubei Province will contribute to the understanding of the epidemiological characteristics and genetic evolution of RVAs in China.
- Group A human rotavirus (RVA)
- , Prevalence
- , Phylogenetic analysis
- , Evolution
- , Hubei Province

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