PREPARATION AND PRELIMINARY APPLICATION OF CYTOMEGALOVIRUS ANTIGEN—GLASS SLIDE TO RAPID DIAGNOSIS

WANG Hui-Min; LUO Lin; GONG Zhen-Kui

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June 1986

WANG Hui-Min, LUO Lin and GONG Zhen-Kui. PREPARATION AND PRELIMINARY APPLICATION OF CYTOMEGALOVIRUS ANTIGEN—GLASS SLIDE TO RAPID DIAGNOSIS[J]. Virologica Sinica, 1986, 1(3).

Citation: WANG Hui-Min, LUO Lin, GONG Zhen-Kui. PREPARATION AND PRELIMINARY APPLICATION OF CYTOMEGALOVIRUS ANTIGEN—GLASS SLIDE TO RAPID DIAGNOSIS .VIROLOGICA SINICA, 1986, 1(3) : 13.

巨细胞病毒抗原片的制备及其在快速诊断中初步应用

1.
湖北省医学科学院
2.
湖北省医学科学院武汉

摘要

本文报道了用巨细胞病毒(CMV,AD_(169)株)感染的人胚肺细胞(HL)制备CMV抗原片。在这种抗原片上进行的间接免疫酶组化法(IPA)初步应用于CMV的快速诊断,并与补体结合试验(CFT)和免疫荧光技术(IFA)进行了比较。检测103份血清中抗CMV IgG抗体和18例双份血清中抗CMV IgG和IgM抗体的结果表明,本方法是特异的,其结果与CFT和IFA的结果一致。这种方法简便、快速、灵敏,不需要特殊的设备,可广泛用于临床诊断和流行病学调查。

PREPARATION AND PRELIMINARY APPLICATION OF CYTOMEGALOVIRUS ANTIGEN—GLASS SLIDE TO RAPID DIAGNOSIS

1.
Hubei Academy of Medical Sciences
2.
Wuhan

Abstract

Cytomegalovirus (CMV) Antigen-glass slide was prepared with human enbryonic lung cells (HL) infected with CMV(AD169 strain). An indirect immunoperoxdase technique (IPA) carried out on the glass slides was preliminary applied to rapid diagnosis of CMV. Complement fixation test (CFT) and immunofluorescence antibody technique (IFA)were run in parallel. The results of detection CMV IgG antibody in 103 sera and CMV IgG and IgM antibody in 18 paired sera showed that IPA is specific, while results of IPA were coin...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

PREPARATION AND PRELIMINARY APPLICATION OF CYTOMEGALOVIRUS ANTIGEN—GLASS SLIDE TO RAPID DIAGNOSIS

1. Hubei Academy of Medical Sciences
2. Wuhan

Abstract: Cytomegalovirus (CMV) Antigen-glass slide was prepared with human enbryonic lung cells (HL) infected with CMV(AD169 strain). An indirect immunoperoxdase technique (IPA) carried out on the glass slides was preliminary applied to rapid diagnosis of CMV. Complement fixation test (CFT) and immunofluorescence antibody technique (IFA)were run in parallel. The results of detection CMV IgG antibody in 103 sera and CMV IgG and IgM antibody in 18 paired sera showed that IPA is specific, while results of IPA were coin...