PRODUCTION OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES TO DENGUE VIRUS TYPE Ⅰ

LIU Le-He; LI Shao-Bing; MAI Xiao-Ping; GUO Hui-Yu; CAI Shang-Da

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February 1987

LIU Le-He, LI Shao-Bing, MAI Xiao-Ping, GUO Hui-Yu and CAI Shang-Da. PRODUCTION OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES TO DENGUE VIRUS TYPE Ⅰ[J]. Virologica Sinica, 1987, 2(1).

Citation: LIU Le-He, LI Shao-Bing, MAI Xiao-Ping, GUO Hui-Yu, CAI Shang-Da. PRODUCTION OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES TO DENGUE VIRUS TYPE Ⅰ .VIROLOGICA SINICA, 1987, 2(1) : 20.

产生抗登革热Ⅰ型病毒单克隆抗体杂交瘤细胞株的建立

1.
中山医科大学
2.
中山医科大学广州

摘要

用登革热Ⅰ型病毒(夏威夷株)兔疫的小白鼠脾细胞与小鼠骨髓瘤细胞融合,获得7个产生抗登革热Ⅰ型病毒单克隆抗体的杂交瘤细胞系,这些杂交瘤细胞上清液及小鼠腹水抗体均用间接兔疫荧光法检测,其中3个杂交瘤细胞系产生的抗体对登革热Ⅰ型病毒具有型特异性。

PRODUCTION OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES TO DENGUE VIRUS TYPE Ⅰ

1.
Zhongshan Medical University
2.
Guangzhou

Abstract

Mouse lymphocyte hybridomas were prepared by polyethylene glycolmediated fusion of ceils from a mouse plasmaeytoma line (X63-Ag8-653) with lymphoeytes from Balb/c mice hyperimmunized with dengue virus type Ⅰ (Hawaii). Seven mouse lymphocyte hybridoma clones(C4, F5, F1, A2, B4, H7,D6) which can steadily produce antibodies against dengue-1 antigen were established after selecting and cloning for at least 3 times and have been kept in the laboratory for more than 5 months. Cross-immunofluorescent staining test...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

PRODUCTION OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES TO DENGUE VIRUS TYPE Ⅰ

1. Zhongshan Medical University
2. Guangzhou

Abstract: Mouse lymphocyte hybridomas were prepared by polyethylene glycolmediated fusion of ceils from a mouse plasmaeytoma line (X63-Ag8-653) with lymphoeytes from Balb/c mice hyperimmunized with dengue virus type Ⅰ (Hawaii). Seven mouse lymphocyte hybridoma clones(C4, F5, F1, A2, B4, H7,D6) which can steadily produce antibodies against dengue-1 antigen were established after selecting and cloning for at least 3 times and have been kept in the laboratory for more than 5 months. Cross-immunofluorescent staining test...