Purification and Characterization of HFRS Virus 50K Structural Protein

XU Zhi-Kai; HONG Mei-Xian; JIANG Chao-Zhun; MA Wen-Yu; WANG Hai-Chao

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April 1988

XU Zhi-Kai, HONG Mei-Xian, JIANG Chao-Zhun, MA Wen-Yu and WANG Hai-Chao. Purification and Characterization of HFRS Virus 50K Structural Protein[J]. Virologica Sinica, 1988, 3(2).

Citation: XU Zhi-Kai, HONG Mei-Xian, JIANG Chao-Zhun, MA Wen-Yu, WANG Hai-Chao. Purification and Characterization of HFRS Virus 50K Structural Protein .VIROLOGICA SINICA, 1988, 3(2) : 124.

肾综合征出血热病毒50K结构蛋白的纯化和鉴定

1.
第四军医大学微生物学教研室
2.
第四军医大学微生物学教研室西安

摘要

以HFRS病毒感染的乳小白鼠脑为原材料,用硫酸鱼精蛋白和PEG沉淀法制备粗病毒抗原。将此粗制抗原通过抗HFRS病毒MeAb与Separose4B偶联制成的免疫吸附柱,并用3MKCNS洗脱,获得了SDS-PAGE均一纯的特异性病毒结构蛋白,分子量为50,000道尔顿,具有较高的抗原滴度和良好的免疫原性。初步认为该蛋白可能是HFRS病毒的一种囊脱蛋白。

Purification and Characterization of HFRS Virus 50K Structural Protein

1.
Department of Microbiology
2.
Fouth Military Medical College

Abstract

A method has been developed to purify HFRS virus structural protein. HFRS virus was first purified roughly by protamine sulfate and polyethylene glycol precipitation from brain tissue of suckling mice infected with HFRS virus strain 76-118 or strain Chen, and the roughly purified virus was then passed to immunoadsorbent column prepared by Sepharose 4B coupled with McAb against HFRS virus. When the column was eluted with potassium thiocyanate a viral protein, it showed only one band on SDS-PAGE and its molec...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Purification and Characterization of HFRS Virus 50K Structural Protein

1. Department of Microbiology
2. Fouth Military Medical College

Abstract: A method has been developed to purify HFRS virus structural protein. HFRS virus was first purified roughly by protamine sulfate and polyethylene glycol precipitation from brain tissue of suckling mice infected with HFRS virus strain 76-118 or strain Chen, and the roughly purified virus was then passed to immunoadsorbent column prepared by Sepharose 4B coupled with McAb against HFRS virus. When the column was eluted with potassium thiocyanate a viral protein, it showed only one band on SDS-PAGE and its molec...