Studies on WMV-2 aud CMV Detection Using Protein A Sandwich ELISA Method

CHEN Yong-Xuan; XUE Bao-Di; LIU Feng-Quan

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August 1988

CHEN Yong-Xuan, XUE Bao-Di and LIU Feng-Quan. Studies on WMV-2 aud CMV Detection Using Protein A Sandwich ELISA Method[J]. Virologica Sinica, 1988, 3(4).

Citation: CHEN Yong-Xuan, XUE Bao-Di, LIU Feng-Quan. Studies on WMV-2 aud CMV Detection Using Protein A Sandwich ELISA Method .VIROLOGICA SINICA, 1988, 3(4) : 357.

A蛋白间接酶联免疫吸附法检测二种西瓜花叶病毒病(WMV-2和CMV)的研究

1.
南京农业大学植病组
2.
南京农业大学植病组南京

摘要

本文用一种改进了的间接酶联免疫吸附法(PAS-ELISA)对二种西瓜上的病毒(WMV-2和CMV)进行检测。结果证明,利用A蛋白顶包被和酶联A蛋白的间接ELISA方法具有高度的灵敏度和特异性。

Studies on WMV-2 aud CMV Detection Using Protein A Sandwich ELISA Method

1.
Nanjing Agricultural University
2.
Dept. of Plant Protection

Abstract

A new form of indirect enzyme-linked immunosorbent assay (ELISA) has been used for detection of WMV-2 and CMV in watermelon plants. The method uses protein A in two applications to direct ELISA method. The first layer of protein A is a precoating layer for the coating antibody. The second layer of protein A is conjugated enzyme for detecting the second antibody layer. In practical application, Protein A sandwich ELISA (PAS-ELISA) was more sensitive and specific than the direct double antibody sandwich form ...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on WMV-2 aud CMV Detection Using Protein A Sandwich ELISA Method

1. Nanjing Agricultural University
2. Dept. of Plant Protection

Abstract: A new form of indirect enzyme-linked immunosorbent assay (ELISA) has been used for detection of WMV-2 and CMV in watermelon plants. The method uses protein A in two applications to direct ELISA method. The first layer of protein A is a precoating layer for the coating antibody. The second layer of protein A is conjugated enzyme for detecting the second antibody layer. In practical application, Protein A sandwich ELISA (PAS-ELISA) was more sensitive and specific than the direct double antibody sandwich form ...