Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus (RMVsh)

TU Zheng-Jin; XU Shan-Qian; ZHANG Re-Ping; WANG Ming-Qi

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August 1988

TU Zheng-Jin, XU Shan-Qian, ZHANG Re-Ping and WANG Ming-Qi. Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus (RMVsh)[J]. Virologica Sinica, 1988, 3(4).

Citation: TU Zheng-Jin, XU Shan-Qian, ZHANG Re-Ping, WANG Ming-Qi. Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus (RMVsh) .VIROLOGICA SINICA, 1988, 3(4) : 364.

长叶车前花叶病毒上海分离株单克隆抗体的制备及其免疫学特性——Ⅰ单克隆抗体制备、亚类测定及免疫沉淀反应

1.
复旦大学生物系
2.
复旦大学生物系上海

摘要

采用杂交瘤技术,以长叶车前花叶病毒上海分离株(RMVsh)为扎原免疫BALB/c小鼠,取脾脏细胞与骨髓瘤SP2/O细胞融合,经筛选克隆,共获得1H2、7H1、10H1、11H2、12H3、17H6、29H17株分泌RMVsh单克隆抗体的杂交瘤细胞株。以双抗夹心ELISA方法测定亚类;1H2属IgG_3,7H1属IgG_(2b),其余均为IgC_1。7个杂交瘤植入鼠体内均产生腹水。在免疫双扩散反应中1H2、12H3能与RMVsh产生免疫沉淀线,而其余5个单克隆抗体均不能与RMVsh产生沉淀线。制备的单克隆抗体可用于病毒病害的诊断鉴定,也可用于进一步分析病毒的抗原特性。

Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus (RMVsh)

1.
Department of Biology
2.
Fudan University

Abstract

Using hybridoma techniques, seven hybridoma cell lines: 1H2, 7H1, 10H1, 11H2, 12H3, 17H6, 29H1, secreting monoclonal antibodies to Shanghai Isolate of Ribgrass Mosaic Virus(RMVsh)were prepared by fusion on myeloma cells SP2/O with spleen cells of BALB/c mouse immunized with RMVsh. The subclasses of these monoclonal antibodies were determinated by double antibody sandwich ELISA. 1H2, 7H1 and other monoclonal antibodies belong to IgG_3, IgG_(2b) and IgG_1 subclass, respectively. The ascitic fluids were obtain...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preparation and Immunological Properties of Monoclonal Antibodies to Ribgrass Mosaic Virus (RMVsh)

1. Department of Biology
2. Fudan University

Abstract: Using hybridoma techniques, seven hybridoma cell lines: 1H2, 7H1, 10H1, 11H2, 12H3, 17H6, 29H1, secreting monoclonal antibodies to Shanghai Isolate of Ribgrass Mosaic Virus(RMVsh)were prepared by fusion on myeloma cells SP2/O with spleen cells of BALB/c mouse immunized with RMVsh. The subclasses of these monoclonal antibodies were determinated by double antibody sandwich ELISA. 1H2, 7H1 and other monoclonal antibodies belong to IgG_3, IgG_(2b) and IgG_1 subclass, respectively. The ascitic fluids were obtain...