Growth Characteristics and High Titer Culture of Grass Carp Hemorrhage Virus (GCHV)-873 in Vitro

FANG Qin; KE Li-Hua; CAI Yi-Quan

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June 1989

FANG Qin, KE Li-Hua and CAI Yi-Quan. Growth Characteristics and High Titer Culture of Grass Carp Hemorrhage Virus (GCHV)-873 in Vitro[J]. Virologica Sinica, 1989, 4(3).

Citation: FANG Qin, KE Li-Hua, CAI Yi-Quan. Growth Characteristics and High Titer Culture of Grass Carp Hemorrhage Virus (GCHV)-873 in Vitro .VIROLOGICA SINICA, 1989, 4(3) : 315.

草鱼出血病病毒的生长特性及高滴价培养

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

草鱼出血病病毒感染鱼肾(Clk)单层细胞后,产生明显的细胞病变,其繁殖周期为2—3天。病毒增殖的上升期在感染后的12—48小时。28℃下,当感染复数(Multiplicityof Infection简称MOI)为0.05PFU/cell时,病毒滴价可达1.78×10~(11)PFU/ml,不同温度(25℃、28℃、30℃)下通气培养,测定病毒滴价略有差异。感染病毒的细胞培养液经ELISA检测和电子显微镜观察均为阳性结果,并且能感染健康草鱼。
- 草鱼出血病病毒
- , 生长特性
- , 病毒效价

Growth Characteristics and High Titer Culture of Grass Carp Hemorrhage Virus (GCHV)-873 in Vitro

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

Some biological properties of Grass Carp Hemorrhage Virus (GCHV)-873have been studied in this report. This virus could make grass carp kidney cells(CIK) to produce a unique cytopathic effect. At 28℃ its logarithmic growthphase occured 12 to 48 hr after infection. The virus titer reached 1.78×10~(11)PFU/mlwhen the MOI was approximately 0.05PFU/cell. There were slight differencesin virus titer under incubation of 25℃, 28℃and 30℃ respectively, and thesimilar results in 5% CO_2 or in normal atmosphere.
- Grass Carp Hemorrhage Virus
- , Growth characteristic
- , Virus titer

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Growth Characteristics and High Titer Culture of Grass Carp Hemorrhage Virus (GCHV)-873 in Vitro

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: Some biological properties of Grass Carp Hemorrhage Virus (GCHV)-873have been studied in this report. This virus could make grass carp kidney cells(CIK) to produce a unique cytopathic effect. At 28℃ its logarithmic growthphase occured 12 to 48 hr after infection. The virus titer reached 1.78×10~(11)PFU/mlwhen the MOI was approximately 0.05PFU/cell. There were slight differencesin virus titer under incubation of 25℃, 28℃and 30℃ respectively, and thesimilar results in 5% CO_2 or in normal atmosphere.