Epitope Analysis of Hemagglutinin Antigen with EHF Virus

WANG Ling-Shu; Ni-Da-Dan; ZHOU Ning

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April 1990

WANG Ling-Shu, Ni-Da-Dan and ZHOU Ning. Epitope Analysis of Hemagglutinin Antigen with EHF Virus[J]. Virologica Sinica, 1990, 5(2).

Citation: WANG Ling-Shu, Ni-Da-Dan, ZHOU Ning. Epitope Analysis of Hemagglutinin Antigen with EHF Virus .VIROLOGICA SINICA, 1990, 5(2) : 125.

流行性出血热病毒血凝素抗原位点分析

1.
安徽省医学科学研究所
2.
安徽省医学科学研究所合肥

摘要

用八株不同来源的流行性出血热(EHF)病毒的单克隆抗体(McAb),采用血凝抑制试验、反向间接血凝抑制试验、间接酶联免疫及阻断酶联免疫试验等,对两种方法(TE、SA)制备的血凝素抗原进行分析。根据A35、2A6McA5试验的结果,证实血凝素抗原中的核蛋白上存在有非构象依赖性的血凝结合位点;而另外5株McAb在血凝抑制活性方面,虽具有明显的株间交叉,但在反向间接血凝抑制试验、间接酶联免疫试验时则均为阴性,故认为其血凝结合位点位于病毒的膜蛋白,可能与G_2糖蛋白有关,为构象依赖性位点。有关4G6McAb,在阻断酶联免疫试验时,虽与A35、2A6一样具有较高的阻断率,但在反向间接血凝抑制试验、间接酶联免疫试验时明显有别于后二者,对其属性有待进一步确定。
- 流行性出血热病毒
- , 单克隆抗体
- , 血凝素
- , 位点分析

Epitope Analysis of Hemagglutinin Antigen with EHF Virus

1.
Anhui Institute of Medical Sciences
2.
Hefei

Abstract

Eight monoclonal antibodies(Mc Abs) from various epidemic hemorrhagic fever(EHF) virus have been used for analyzing the epitopes of SA and TE hemagglutinin antigens by hemagglutination inhibition(HI), reversed passive hemmagglutination inhibition(RPHI), indirect ELISA and blocking ELISA.It was demonstrated that there were conformation independing hemagglutination binding epitopes in nucleocapsid protein by A_(35)、2A_6 McAb. Although other five strains of McAbs possessed obvious crosses in the activity of he...
- Epidemic hemorrhagic fever virus
- , Monoclonal antibody
- , Hemagglutinin
- , Epitope analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Epitope Analysis of Hemagglutinin Antigen with EHF Virus

1. Anhui Institute of Medical Sciences
2. Hefei

Abstract: Eight monoclonal antibodies(Mc Abs) from various epidemic hemorrhagic fever(EHF) virus have been used for analyzing the epitopes of SA and TE hemagglutinin antigens by hemagglutination inhibition(HI), reversed passive hemmagglutination inhibition(RPHI), indirect ELISA and blocking ELISA.It was demonstrated that there were conformation independing hemagglutination binding epitopes in nucleocapsid protein by A_(35)、2A_6 McAb. Although other five strains of McAbs possessed obvious crosses in the activity of he...