The Preparation of Monoclonal Anti-idiotypic Antibodies Against Mouse Monoclonal Anti-HBs and Their Immunogenicity Ⅱ.The Study on Immunogenicity of Ab_2-1

WANG Hai-Chao; Chen Wan-Rong; JIANG Yu-Tu

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April 1990

WANG Hai-Chao, Chen Wan-Rong and JIANG Yu-Tu. The Preparation of Monoclonal Anti-idiotypic Antibodies Against Mouse Monoclonal Anti-HBs and Their Immunogenicity Ⅱ.The Study on Immunogenicity of Ab_2-1[J]. Virologica Sinica, 1990, 5(2).

Citation: WANG Hai-Chao, Chen Wan-Rong, JIANG Yu-Tu. The Preparation of Monoclonal Anti-idiotypic Antibodies Against Mouse Monoclonal Anti-HBs and Their Immunogenicity Ⅱ.The Study on Immunogenicity of Ab_2-1 .VIROLOGICA SINICA, 1990, 5(2) : 162.

鼠单克隆抗-HBs的单克隆抗独特型抗体的制备及其免疫原性的研究 Ⅱ.Ab_2—1的免疫原性研究

1.
军事医学科学院微生物流行病研究所
2.
军事医学科学院微生物流行病研究所北京

摘要

用一株单克隆抗独特型抗体细胞株,Ab_2-1免疫4只BALB/c小鼠和2只家兔。每只小鼠每次经腹腔接种100#gAb_2-1,共接种4次;每只家兔每次经皮下注射1mgAb_2-1,共注射4次。经免疫的小鼠和家兔都产生了抗-HBs,经两种竞争性抑制试验证实,这些抗-HBs是特异性的。小鼠抗-HBs滴度为2~(-6)至2~(-9);家兔抗-HBs滴度从2~(-10)至2~(-12)。这些资料提示:单克隆抗独特型抗体能模拟HBsAg,具有类似HBsAg的免疫原性。
- 抗独特型抗体
- , 单克隆抗体
- , 乙型肝炎疫苗

The Preparation of Monoclonal Anti-idiotypic Antibodies Against Mouse Monoclonal Anti-HBs and Their Immunogenicity Ⅱ.The Study on Immunogenicity of Ab_2-1

1.
Institute of Microbiology and Epidemiology Academy of Military Medical Sciences
2.
Beijing 100071

Abstract

The Ab_2-1 was used to immunize four BALB/c mice and two rabbits. Each mouse recievcd inoculation of 100μg of Ab_2-1 for four times intraperitoneally. Each rabbit was injected with 1 mg of Ab_2-1 for four times subctaneously. All the mice and rabbits immunized with Ab_2-1 produced anti-HBs antibodies, specificity of which also was confirmed by two types of competitive inhibition test(SPRIA). The titers of mouse anti-HBs were from 2~(-6) to 2~(-9) the anti-HBs titers of rabbit sera varied from 2~(-10) to 2~(...
- Anti-idiotypic antibodies
- , Monoclonal antibodies
- , HB vaccines

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

The Preparation of Monoclonal Anti-idiotypic Antibodies Against Mouse Monoclonal Anti-HBs and Their Immunogenicity Ⅱ.The Study on Immunogenicity of Ab_2-1

1. Institute of Microbiology and Epidemiology Academy of Military Medical Sciences
2. Beijing 100071

Abstract: The Ab_2-1 was used to immunize four BALB/c mice and two rabbits. Each mouse recievcd inoculation of 100μg of Ab_2-1 for four times intraperitoneally. Each rabbit was injected with 1 mg of Ab_2-1 for four times subctaneously. All the mice and rabbits immunized with Ab_2-1 produced anti-HBs antibodies, specificity of which also was confirmed by two types of competitive inhibition test(SPRIA). The titers of mouse anti-HBs were from 2~(-6) to 2~(-9) the anti-HBs titers of rabbit sera varied from 2~(-10) to 2~(...