Comparison of 3 Methods of Concentration of Poliovirus in Water Samples and Establishment of an Improved Method

YOU Feng-Xin; LIU Bing-Hui; ZHANG Ling-Mei; DING Mao-Jin

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April 1991

YOU Feng-Xin, LIU Bing-Hui, ZHANG Ling-Mei and DING Mao-Jin. Comparison of 3 Methods of Concentration of Poliovirus in Water Samples and Establishment of an Improved Method[J]. Virologica Sinica, 1991, 6(2).

Citation: YOU Feng-Xin, LIU Bing-Hui, ZHANG Ling-Mei, DING Mao-Jin. Comparison of 3 Methods of Concentration of Poliovirus in Water Samples and Establishment of an Improved Method .VIROLOGICA SINICA, 1991, 6(2) : 125.

水标本中脊髓灰质炎病毒三种浓缩方法的比较及改进方法的建立

1.
无锡市卫生防疫站
2.
无锡市卫生防疫站无锡

摘要

本文用三种浓缩方法对人工污染标本进行了病毒回收率的比较试验,三种方法结果基本相同(47—49％)。在被检脊髓灰质炎Ⅰ型病毒蒸馏水标本中加1％NaCl可提高滤膜法对水中病毒的回收率;在含脊髓灰质炎病毒的河水、污水标本中加1％NaCl及AlCl_3(河水为0.0005mol/L、污水为0.001mol/L),离心沉淀过程中,我们惊奇地发现病毒全在沉渣中,结果表明用该方法病毒回收率竟提高了一倍(92—99％)。
- 水病毒检测
- , 水中病毒浓缩
- , 脊髓灰质炎病毒

Comparison of 3 Methods of Concentration of Poliovirus in Water Samples and Establishment of an Improved Method

1.
Public Health and Preventative Station
2.
Wuxi

Abstract

This paper described the quantative eomprison of 3 methods concentrating poliovirus type 1 artificially added into the water samples, The preliminary results showed that the recovery rate of the virus in the water samples was similar (47—49％)among the 3 methods, It was discovered that the addition of 1％ NaCl into virus contaminted distilled water sample increased significantly the virus-recovery rate with milliporous membrane filtrating method and we established an improved process of virus concentration of...
- Detection of water virus
- , Concentration of water
- , Poliovirus type 1

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparison of 3 Methods of Concentration of Poliovirus in Water Samples and Establishment of an Improved Method

1. Public Health and Preventative Station
2. Wuxi

Abstract: This paper described the quantative eomprison of 3 methods concentrating poliovirus type 1 artificially added into the water samples, The preliminary results showed that the recovery rate of the virus in the water samples was similar (47—49％)among the 3 methods, It was discovered that the addition of 1％ NaCl into virus contaminted distilled water sample increased significantly the virus-recovery rate with milliporous membrane filtrating method and we established an improved process of virus concentration of...