Surface Contamination of Soybean Pollen by SMV and Effect of Virus on Pollen Activity

TAO Ting-Dian; PU Zu-Qin; PU Zu-Mao

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June 1991

TAO Ting-Dian, PU Zu-Qin and PU Zu-Mao. Surface Contamination of Soybean Pollen by SMV and Effect of Virus on Pollen Activity[J]. Virologica Sinica, 1991, 6(3).

Citation: TAO Ting-Dian, PU Zu-Qin, PU Zu-Mao. Surface Contamination of Soybean Pollen by SMV and Effect of Virus on Pollen Activity .VIROLOGICA SINICA, 1991, 6(3) : 232.

SMV对大豆花粉表面的污染及对花粉活力的影响

1.
南京农业大学植物病理教研室
2.
中国药科大学电镜室南京 210014上海动植物检疫所

摘要

应用PALLAS将病毒粒体转化为乳胶颗粒通过扫描电镜直接观察,成功地检测到了大豆花粉表面污染的大豆花叶病毒(SMV)。田间春大豆宁镇1号的重花叶、轻花叶及无症状植株花粉所吸附的乳胶颗粒依次递减,而健株花粉上极少观测到乳胶颗粒,花粉数量测定及萌发试验表明,SMV的侵染对大豆花粉形成及活力都有明显的影响。
- 大豆花叶病毒
- , 花粉
- , PALLAS
- , 扫描电镜
- , 花粉萌发

Surface Contamination of Soybean Pollen by SMV and Effect of Virus on Pollen Activity

1.
Department of Plant Protection
2.
Nanjing Agricultural University

Abstract

The particles of soybean mosaic virus on the surface of soybean pollen were detected by protein A-linked latex antiserum(PALLAS)with scanning microscope When soybean variety of Ning Zhen -1 was used as material, the latex beads on pollen from severe mosaic, light mosaic and symptomless soybean plants were about 1000, 200 and 15 respectively, but no latex beads were observed on the surface of pollen from the healthy one. Pollen germination experiment shows that the pollen activity is obviously reduced becaus...
- Soybean mosaic:virus Pollen PALLAS Scanning electron microscopy Pollcn germination

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Surface Contamination of Soybean Pollen by SMV and Effect of Virus on Pollen Activity

1. Department of Plant Protection
2. Nanjing Agricultural University

Abstract: The particles of soybean mosaic virus on the surface of soybean pollen were detected by protein A-linked latex antiserum(PALLAS)with scanning microscope When soybean variety of Ning Zhen -1 was used as material, the latex beads on pollen from severe mosaic, light mosaic and symptomless soybean plants were about 1000, 200 and 15 respectively, but no latex beads were observed on the surface of pollen from the healthy one. Pollen germination experiment shows that the pollen activity is obviously reduced becaus...