Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization

YANG Tie-Qiu; LI Zhi-Hong; YANG Beng; LIU Wei-Qian; DIAO Wen-Xian; LI De-Xin; HANG Chang-Shou

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August 1991

YANG Tie-Qiu, LI Zhi-Hong, YANG Beng, LIU Wei-Qian, DIAO Wen-Xian, LI De-Xin and HANG Chang-Shou. Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization[J]. Virologica Sinica, 1991, 6(4).

Citation: YANG Tie-Qiu, LI Zhi-Hong, YANG Beng, LIU Wei-Qian, DIAO Wen-Xian, LI De-Xin, HANG Chang-Shou. Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization .VIROLOGICA SINICA, 1991, 6(4) : 320.

斑点杂交生物素法检测流行性出血热病毒RNA

1.
湖北医学院病毒研究所
2.
中国预防医学科学院病毒研究所
3.
中国预防医学科学院病毒研究所武汉

摘要

为寻找一种用于检测流行性出血热病毒的分子杂交方法,以生物素-7-dATP标记流行性出血热病毒(EHFV)R_(22)株M片段的cDNAR_3克隆作探针,与人源性的EHFVH-114、H-435株RNA基因组进行斑点杂交,得到阳性结果,可检出5pg的cDNA或RNA。此探针与疱疹病毒DNA不出现杂交信号。以上结果说明这种标记探针具有EHFV特异性,可以扩大应用范围,结果还表明动物源性和人源性EHFV均具有共同的保守核苷酸序列。
- 生物素
- , 出血热病毒
- , RNA基因组
- , 杂交技术

Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization

1.
Virus Research Institute
2.
Hubei Medical College
3.
Wuhan

Abstract

Dot hybridization assay was developed to detect epidemic hemorrhagic fever virus(EHFV)RNA genome using probe which biotin-7-dATP labeled R-3 cDNA clone of M fragement of EHFV R-22 strain. This probe was used to detect RNA genome of the human original EHFV H-114 and H-435 strains in cell cultures. The positive hybridization signal was obtained from cells infected EHFV H-114 and H-435 strains, 5 pg cDNA or RNA could be obtained This probe do not react with DNA of cells infected herpes simplex virus and cytome...
- Biotin Hantanvirus RNA genome Dot hybridization

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Epidemic Hemorrhagic Fever Virus (Hantanvirus) RNA Genome with Biotin-Labeled Probe by Dot Hybridization

1. Virus Research Institute
2. Hubei Medical College
3. Wuhan

Abstract: Dot hybridization assay was developed to detect epidemic hemorrhagic fever virus(EHFV)RNA genome using probe which biotin-7-dATP labeled R-3 cDNA clone of M fragement of EHFV R-22 strain. This probe was used to detect RNA genome of the human original EHFV H-114 and H-435 strains in cell cultures. The positive hybridization signal was obtained from cells infected EHFV H-114 and H-435 strains, 5 pg cDNA or RNA could be obtained This probe do not react with DNA of cells infected herpes simplex virus and cytome...