Cucumber Mosaic Virus Coat Protein Inside the Chloroplast of the Infected Tobacco

ZHU Shui-Fang; R.I.B. Francki

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June 1992

ZHU Shui-Fang and R.I.B. Francki. Cucumber Mosaic Virus Coat Protein Inside the Chloroplast of the Infected Tobacco[J]. Virologica Sinica, 1992, 7(3).

Citation: ZHU Shui-Fang, R.I.B. Francki. Cucumber Mosaic Virus Coat Protein Inside the Chloroplast of the Infected Tobacco .VIROLOGICA SINICA, 1992, 7(3) : 328.

黄瓜花叶病毒衣壳蛋白存在于被其侵染的烟草叶绿体中

1.
农业部植物检疫实验所
2.
澳大利亚Adelaide大学北京

摘要

用原生质体法制备出高纯度的完整叶绿体经SDS-PAGE电泳,银染后,发现黄瓜花叶病毒(CMV)侵染的烟草病叶叶绿体蛋白质图谱和健叶叶绿体相比,多出一条染色较弱的迁移率与CMV衣壳蛋白质相同的带,经Western转移,用CMV游离衣壳蛋白亚基抗血清进行斑点酶联(Immunoblot)检测,证明这条带就是CMV衣壳蛋白质。健康叶绿体中加入去掉叶绿体的病叶汁液而制备出的叶绿体中无CMV衣壳蛋白质,说明这不是在叶绿体提纯过程中得到的假象,即衣壳蛋白质存在于被CMV侵染的完整叶片叶绿体中。这个结果否认了以往报道的CMV衣壳蛋白质不存在于烟草叶绿体中的结论。另外还发现,叶绿体中的衣壳蛋白质浓度与叶片症状严重程度呈正相关。
- 黄瓜花叶病毒
- , 衣壳蛋白质
- , 叶绿体
- , 烟草
- , 斑点酶联

Cucumber Mosaic Virus Coat Protein Inside the Chloroplast of the Infected Tobacco

1.
Plant Quarantine Inst. Beijing 100026Adelaide Univ. Australia 5064

Abstract

The intact chloroplasts were made through protoplasts. In the SDSPAGE-silver stained gel, one extra protein band, which had the same mobility and molecular weight as the cucumber mosaic virus coat protein (CMV-cp), was found in the diseased chloroplast comparative to the health chloroplast. In the immunoblot testing, the band was highly reactive to the antiserum against the free CMV-cp, while the healthy and diseased sap treated healthy chloroplasts had no reaction. The result provides an evidence for the C...
- CMV Coat protein Chloroplast Tobacco Immunoblot

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cucumber Mosaic Virus Coat Protein Inside the Chloroplast of the Infected Tobacco

1. Plant Quarantine Inst. Beijing 100026Adelaide Univ. Australia 5064

Abstract: The intact chloroplasts were made through protoplasts. In the SDSPAGE-silver stained gel, one extra protein band, which had the same mobility and molecular weight as the cucumber mosaic virus coat protein (CMV-cp), was found in the diseased chloroplast comparative to the health chloroplast. In the immunoblot testing, the band was highly reactive to the antiserum against the free CMV-cp, while the healthy and diseased sap treated healthy chloroplasts had no reaction. The result provides an evidence for the C...