Detection and Significance of Antiidiotype Antibodies against Anti-HBs in Immune Complexes of Patients with Hepatitis B

WANG Ai-Li; WU Jian-Guo

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August 1992

WANG Ai-Li and WU Jian-Guo. Detection and Significance of Antiidiotype Antibodies against Anti-HBs in Immune Complexes of Patients with Hepatitis B[J]. Virologica Sinica, 1992, 7(4).

Citation: WANG Ai-Li, WU Jian-Guo. Detection and Significance of Antiidiotype Antibodies against Anti-HBs in Immune Complexes of Patients with Hepatitis B .VIROLOGICA SINICA, 1992, 7(4) : 414.

乙肝患者抗HBs独特型抗体特异性免疫复合物的检测与意义

王艾丽 ,
武建国

1.
南京军区南京总医院临床免疫科
2.
南京军区南京总医院临床免疫科南京

摘要

根据用终浓度分别为35.0g/L和17.5g/L聚乙二醇沉淀循环免疫复合物,去除游离抗HBs-Ab_2,再以胰蛋白酶解离复合物的原理,建立了检测抗HBs-Ab_2-ICs的ELISA法。结果表明,38例急性乙型肝类和83例慢性活动性乙肝患者的IgG、IgM类抗HBs-Ab_2-ICs总阳性率分别为13.2％(5/38)和18.1％(15/83)。IgG、IgM类抗HBs-Ab_2-ICs检出率无显著差异(P>0.05)。实验证实乙肝患者体内存在含抗HBs-Ab_2-ICs。提示抗HBs-Ab_2尚可与抗HBs结合,抑制其中和HBV的作用而利于HBV复制。
- 抗HBs-Ab
- , 免疫复合物
- , 乙肝患者
- , PEG Trypsin ELISA

Detection and Significance of Antiidiotype Antibodies against Anti-HBs in Immune Complexes of Patients with Hepatitis B

WANG Ai-Li ,
WU Jian-Guo

1.
Department of Clinical Immunology
2.
Naniing Hospital of Nanjing Command

Abstract

An enzyme linked immunosorbent assay for detecting antiidiotype antibodies against anti-HBs-containing immune complexes (antiHBs-Ab_2IC)in hepatitis B patients has been established, which was based on precipitating IG by 35.0g/L and 17.5g/L polyethyene glycol(PEG, MW 6000) and separating IC by trypsin, Our results showed that the positive rates of antiHBs-Ab_2-IC in the patients with hepatitis B(n=38)and chronic hepatitis B(n=83)were 13o2% (5/38)and 18.1% (15/83)respectively. There was on statistical differ...
- AntiHBs-Ab
- , Hepatitis B patients
- , PEC Trypsin ELISA

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection and Significance of Antiidiotype Antibodies against Anti-HBs in Immune Complexes of Patients with Hepatitis B

WANG Ai-Li ,
WU Jian-Guo

1. Department of Clinical Immunology
2. Naniing Hospital of Nanjing Command

Abstract: An enzyme linked immunosorbent assay for detecting antiidiotype antibodies against anti-HBs-containing immune complexes (antiHBs-Ab_2IC)in hepatitis B patients has been established, which was based on precipitating IG by 35.0g/L and 17.5g/L polyethyene glycol(PEG, MW 6000) and separating IC by trypsin, Our results showed that the positive rates of antiHBs-Ab_2-IC in the patients with hepatitis B(n=38)and chronic hepatitis B(n=83)were 13o2% (5/38)and 18.1% (15/83)respectively. There was on statistical differ...