Detection of HCV-RNA in Plasmas of Blood Donors by Nested Double Polymerase Chain Reaction Method——And the Evaluation of Anti-HCV Antibody Test as a Blood Donation Screening Assay

TU Jun-Ping; SEN Tie-Nan; SHEN Tian-Qian-Er

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February 1993

TU Jun-Ping, SEN Tie-Nan and SHEN Tian-Qian-Er. Detection of HCV-RNA in Plasmas of Blood Donors by Nested Double Polymerase Chain Reaction Method——And the Evaluation of Anti-HCV Antibody Test as a Blood Donation Screening Assay[J]. Virologica Sinica, 1993, 8(1).

Citation: TU Jun-Ping, SEN Tie-Nan, SHEN Tian-Qian-Er. Detection of HCV-RNA in Plasmas of Blood Donors by Nested Double Polymerase Chain Reaction Method——And the Evaluation of Anti-HCV Antibody Test as a Blood Donation Screening Assay .VIROLOGICA SINICA, 1993, 8(1) : 65.

Nested double PCR法对献血者血浆中HCV-RNA的检出——兼评作为献血筛选试验的Anti-HCV抗体检查

1.
武汉市中心血站
2.
日本国福冈县赤十字血液中心
3.
日本国福冈县赤十字血液中心武汉
4.
日本 818

摘要

本文报道了在位于5’端非编码区的引物的诱导下用Nested double PCR技术检测献血者血浆中HCV-RNA的方法。我们发现Nested double PCR敏感性及特异性均优于单次PCR。anti-HCV(C100-3)阳性血浆样品中只有35.2％(19/54)能同时被证实为PCR阳性。由于anti-HCV(C100-3)假阳性率太高,作为献血筛选试验检测方法不能令人满意,而本文报导的Nestel double PCR方法可以弥补anti-HCV试验的不足。
- HCV
- , 献血者
- , HCV基因
- , anti-HCV抗体（C100-3）
- , Nested double PCR

Detection of HCV-RNA in Plasmas of Blood Donors by Nested Double Polymerase Chain Reaction Method——And the Evaluation of Anti-HCV Antibody Test as a Blood Donation Screening Assay

1.
Wuhan Blood Traijsfusicn Center
2.
Wuhan 430030Japanese Red Cross Fukuoka Blood Center
3.
Japan 818

Abstract

In this paper we reported the detection of HCV-RNA in plasma of blood donors by nested double polymerase chain reaction method using two pairs of primers located in the 5 -terminal non-coding region. We found that nested double PCR method is much better than single PCR method both in sensitivity and specificity. And we also found that only 35.2% (19/54)of anti-HCV(C100-3)positive plasma samples can be confirmed to be also PCR positve. We concluded that because the false positve rate of anti-HCV ELISA is too...
- HCV
- , Blood donors
- , HCV-genome
- , Anti-HCV antibody （ C100-3 ）
- , Nested double PCR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of HCV-RNA in Plasmas of Blood Donors by Nested Double Polymerase Chain Reaction Method——And the Evaluation of Anti-HCV Antibody Test as a Blood Donation Screening Assay

1. Wuhan Blood Traijsfusicn Center
2. Wuhan 430030Japanese Red Cross Fukuoka Blood Center
3. Japan 818

Abstract: In this paper we reported the detection of HCV-RNA in plasma of blood donors by nested double polymerase chain reaction method using two pairs of primers located in the 5 -terminal non-coding region. We found that nested double PCR method is much better than single PCR method both in sensitivity and specificity. And we also found that only 35.2% (19/54)of anti-HCV(C100-3)positive plasma samples can be confirmed to be also PCR positve. We concluded that because the false positve rate of anti-HCV ELISA is too...