Studies on Liver Biopsy of Epidemic Hemorrhagic Fever by in Situ Hybridization and Immunohistochemistry

WANG Chun-Jie; DENG Beng-Fei; ZHANG Wen; LAI Jia-Qi; LIU Feng-Ge

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June 1993

WANG Chun-Jie, DENG Beng-Fei, ZHANG Wen, LAI Jia-Qi and LIU Feng-Ge. Studies on Liver Biopsy of Epidemic Hemorrhagic Fever by in Situ Hybridization and Immunohistochemistry[J]. Virologica Sinica, 1993, 8(3): 213-217.

Citation: WANG Chun-Jie, DENG Beng-Fei, ZHANG Wen, LAI Jia-Qi, LIU Feng-Ge. Studies on Liver Biopsy of Epidemic Hemorrhagic Fever by in Situ Hybridization and Immunohistochemistry .VIROLOGICA SINICA, 1993, 8(3) : 213-217.

应用原位杂交及免疫组化检测肝活检组织中流行性出血热病毒RNA及抗原

1.
中国人民解放军白求恩国际和平医院
2.
石家庄市传染病院
3.
石家庄市传染病院石家庄
4.
050082 西安第四军医大学病理教研室博士研究生
5.
050082
6.
050054

摘要

本文应用原位杂交及免疫组化技术对25例流行性出血热(EHF)患者肝活检组织进行了病毒RNA及其囊膜G2蛋白的定位检测,结合光镜,认为肝细胞的变性、胞浆疏松化、点状坏死是EHF病毒直接侵犯并在其胞浆内增殖表达所致,肝组织的灶状坏死则主要是肝窦狭窄、枯否氏细胞增生导致微循环障碍引起的缺血性梗死。急性脂褐素沉积是病毒侵犯肝细胞的间接证据。研究还发现,肝细胞内病毒的多少与病程关系不明确,而与临床分型有一定相关,为探讨EHF的发病机制提供了分子水平的依据。
- 流行性出血热
- , RNA
- , 抗原
- , 肝活检
- , 杂交
- , 免疫组化

Studies on Liver Biopsy of Epidemic Hemorrhagic Fever by in Situ Hybridization and Immunohistochemistry

1.
Bethune International Peace Hospital
2.
Shijiazhuang
3.
050082

Abstract

The EHF RNA and its G2 antigen were detected by in situ hybridization and immunohistoche- mistry on liver biopsy specimens of 25 cases of EHF patients among whom 23 are postive. Our results indicated that the degeneration and the single hepatocyte necrosis were due to the EHF virus invading hepatocytes directly and replicating in their cytoplasm; the local hepatocyte necrosis may be a kind of ischemia infarct which was mainly because of micro-circulation disturbance owing to the narrow of heaptocellular si...
- Epidemic Hemorrhagic Fever
- , RNA Antigen
- , Hybridization
- , Liver biopsy
- , Immunohistochemistry

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on Liver Biopsy of Epidemic Hemorrhagic Fever by in Situ Hybridization and Immunohistochemistry

1. Bethune International Peace Hospital
2. Shijiazhuang
3. 050082

Abstract: The EHF RNA and its G2 antigen were detected by in situ hybridization and immunohistoche- mistry on liver biopsy specimens of 25 cases of EHF patients among whom 23 are postive. Our results indicated that the degeneration and the single hepatocyte necrosis were due to the EHF virus invading hepatocytes directly and replicating in their cytoplasm; the local hepatocyte necrosis may be a kind of ischemia infarct which was mainly because of micro-circulation disturbance owing to the narrow of heaptocellular si...