将草鱼出血病病毒（ＧｒａｓｓＣａｒｐＨｅｍｏｒｒｈａｇｅＶｉｒｕｓ，ＧＣＨＶ）置于还原性的溶液中，然后加入等体积的ＮａＨＣＯ３配制的异硫氰酸荧光索溶液进行多肽的标记，再经ＳＤＳ－ＰＡＧＥ分析，在紫外灯下即可检测到ＧＣＨＶ全部的１１个结构多肽的荧光带。该方法最小检测量为５００ｎｇ，由该方法回收的多肽具有抗原活性，可作为抗原进行免疫学实验。

t had been reported that the GCHV(Gtass Carp Hemorrhage Virus)is a new member.in Reoviri-dae and has 11 polypeptides by our laboratory,We did further experiments on the viral polypeptides.The virions(GCHV-875)were purified from the medium of infection culture cells by CsCl density gradient centrifugation,dissolved in the buffer containing Tris-HCl 250mmol/L,glycerin 25%,SDS86mmol/L,2-ME 320mmol/L(pH6.9)and then heated at 100℃for 3 min.After that,by addingan equal volume of FITC solution(2mg/M)in 1 mol/L NaHCO3,the viral polypeptides were labelledwith FITC,and analysed by SDS-PAGE. All the viral structural polypeptides(11 bands),namelyVP130,VP120,VP113,VP107,VP75,VP65,VP60,VP52,VP42,VP39 and VP31,wete visible under. UV light.These polypeptides were retrieved by cutting down each band from the gel and demonstrated kept immunological property.The method can determine 500ng protein band in gel under UVlight.