CHEN Dong, XIE Tian-En and JIN Feng. Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene[J]. Virologica Sinica, 1994, 9(3).
Citation:
CHEN Dong, XIE Tian-En, JIN Feng.
Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene .VIROLOGICA SINICA, 1994, 9(3)
: 217.
黄地老虎颗粒体病毒DNA片段的克隆与颗粒体蛋白基因的定位
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中国科学院武汉病毒研究所 中国科技大学生物系
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摘要
用pUC19质粒作载体,克隆了黄地老虎颗粒体病毒(Agrolissegetumgranulosisvirus,简称AsGV)DNAPstI-D.E.F.G.H.J.K.等7个片段。以[ ̄(32)P]-dCTP标记的油桐尺蠖核型多角体病毒(Buzurasuppressarianuclcarpolyhedrosisvirus简称BsNPV)多角体蛋白基因为探针,在37℃条件下对AsGV)颗粒体蛋白基因进行了定位,将其分别定位于BslⅡ-S或TPsTI-A或B和EciRI-A片段上。
Cloning of restriction fragments from Agrotis segetum granulosis virus DNA and localization of the gran ulin gene
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Abstract
Agrotis segetum granulosis virus (AsGV) is an important biolosical control agent for A.segelum.Several PstI fragments from AsGV were cloned into pUC19.Under the very low strinsency(20% formamide,37℃ ),we have leealized the granulin sene on BglII-S or T,EcoRI-A,PstI-A or B frag.ments of AsGV DNA,by using the 32P-labelled polyhedrin gene of Buzura suppressaria nuclear polyhedrosis virus (BsNPV)as a prcbe.
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References
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Proportional views
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