Construction of Pseudorabies Virus Genomic Library and Analysis of Physical Mapping

WANG Ming-Shu; GUO Wan-Zhu; LOU Gao-Ming; BI En-Ge; XUAN Hua

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August 1994

WANG Ming-Shu, GUO Wan-Zhu, LOU Gao-Ming, BI En-Ge and XUAN Hua. Construction of Pseudorabies Virus Genomic Library and Analysis of Physical Mapping[J]. Virologica Sinica, 1994, 9(4).

Citation: WANG Ming-Shu, GUO Wan-Zhu, LOU Gao-Ming, BI En-Ge, XUAN Hua. Construction of Pseudorabies Virus Genomic Library and Analysis of Physical Mapping .VIROLOGICA SINICA, 1994, 9(4) : 347.

伪狂犬病毒闽A株基因文库的构建及物理图谱分析

1.
四川农业大学中国人民解放军农牧大学

摘要

本文报道以质粒ｐＢＲ３２２作载体，用鸟枪法克隆出了ＰＲＶ闽Ａ株除ＢａｍＨＩ－１，２外的所有酶切片段，构建了ＰＲＶ闽Ａ株基因文库，并以克隆出的ＢａｍＨＩ片段用光生物素标记作探针，应用分子杂交法确定了ＰＲＶ闽Ａ株绝大部分限制性内切酶位点的位置。
- 伪狂犬病毒
- , 基因文库
- , 物理图谱

Construction of Pseudorabies Virus Genomic Library and Analysis of Physical Mapping

1.
Sichuan Agricultural University

Abstract

The Restriction endonuclease fragments except the ones from BamH I-1,2 were inserted into plasmid pBR322.The genomic library of Pseudurabies virus Min-A was also constructed.The Cloned frag-ment degested by BamH I were ldbeled with pliotobiotin,With molecular hybridization,the maincleavage site of Pseudorabies virus Min-A strain were determined.
- Pesudurables virus Genomic library Physical mapping

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction of Pseudorabies Virus Genomic Library and Analysis of Physical Mapping

1. Sichuan Agricultural University

Abstract: The Restriction endonuclease fragments except the ones from BamH I-1,2 were inserted into plasmid pBR322.The genomic library of Pseudurabies virus Min-A was also constructed.The Cloned frag-ment degested by BamH I were ldbeled with pliotobiotin,With molecular hybridization,the maincleavage site of Pseudorabies virus Min-A strain were determined.