Nucleotide Sequence Analysis of the Polyhedrin Gene of Heliothis armigera Single Nucleocapsid Nucleopolyhedrovirus

CHEN Xin-Wen; HU Zhi-Hong; JustM.Vlak

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August 1997

CHEN Xin-Wen, HU Zhi-Hong and JustM.Vlak. Nucleotide Sequence Analysis of the Polyhedrin Gene of Heliothis armigera Single Nucleocapsid Nucleopolyhedrovirus[J]. Virologica Sinica, 1997, 12(4).

Citation: CHEN Xin-Wen, HU Zhi-Hong, JustM.Vlak. Nucleotide Sequence Analysis of the Polyhedrin Gene of Heliothis armigera Single Nucleocapsid Nucleopolyhedrovirus .VIROLOGICA SINICA, 1997, 12(4) : 346.

中国棉铃虫单粒包埋核型多角体病毒多角体蛋白基因的序列分析

1.
中国科学院武汉病毒研究所

摘要

以AcMNPV的多角体蛋白基因为探针，定位了中国棉铃虫单粒包理核型多角体病毒（HaSNPV）的多用体蛋白基因。序列测定表明，HaSNPV的多角体蛋白基因编码区为738个核苷酸，编码246个氨基酸，预计蛋白质分子量为29kDa。同源性分析表明，HaSNPV与美洲棉铃虫单粒包理核型多角体病毒（HzSNPV）具有最高的同源性，在整个阅读框架中只有4个碱基的差异，其中第179位碱基的变化导致唯一的氨基酸变化，这种变化并未导致其二级结构的改变。此外，两种病毒该基因的启动子结构也完全一样。HaSNPV与其它的SNPV和MNPV的同源性明显要低。结果预示HaSNPV与HzSNPV可能为同种病毒的不同变种。
- 中国棉铃虫单粒包埋核型多角体病毒
- , 多角体蛋白基因
- , 核苷酸序列

Nucleotide Sequence Analysis of the Polyhedrin Gene of Heliothis armigera Single Nucleocapsid Nucleopolyhedrovirus

1.
Wuhan Institute of Virology

Abstract

The polyhedrin gene of the Heliothis armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) was localized by Southern blot hybridization with a probe from the Autographa cali-fornica MNPV (AcMNPV) polyhedrin gene. Nucleotide sequencing showed the open reading frame of the HaSNPV polyhedrin gene was 738 nt, encoded 246 amino acids with a predicted size of 29 kDa. Alignment with polyhedrin sequences in GENE Band indicated the highest homology with the polyhedrin gene of H. zea SNPV(HzSNPV). There were fou...
- Heliothis armigera SNPV
- , Polyhedrin gene
- , Nucleotide sequence

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Nucleotide Sequence Analysis of the Polyhedrin Gene of Heliothis armigera Single Nucleocapsid Nucleopolyhedrovirus

1. Wuhan Institute of Virology

Abstract: The polyhedrin gene of the Heliothis armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) was localized by Southern blot hybridization with a probe from the Autographa cali-fornica MNPV (AcMNPV) polyhedrin gene. Nucleotide sequencing showed the open reading frame of the HaSNPV polyhedrin gene was 738 nt, encoded 246 amino acids with a predicted size of 29 kDa. Alignment with polyhedrin sequences in GENE Band indicated the highest homology with the polyhedrin gene of H. zea SNPV(HzSNPV). There were fou...