Comparison and Analysis on HCV NS5 Gene Fragment between GenotypeⅡ isolates from Same Region

XU Dong-Gang; DAI Hao-Ying; NIU Jian-Zhang; SHU Ban-Lu; MENG Qiang-Hua; MENG Zong-Da

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February 1998

XU Dong-Gang, DAI Hao-Ying, NIU Jian-Zhang, SHU Ban-Lu, MENG Qiang-Hua and MENG Zong-Da. Comparison and Analysis on HCV NS5 Gene Fragment between GenotypeⅡ isolates from Same Region[J]. Virologica Sinica, 1998, 13(1).

Citation: XU Dong-Gang, DAI Hao-Ying, NIU Jian-Zhang, SHU Ban-Lu, MENG Qiang-Hua, MENG Zong-Da. Comparison and Analysis on HCV NS5 Gene Fragment between GenotypeⅡ isolates from Same Region .VIROLOGICA SINICA, 1998, 13(1) : 54.

同型HCV不同分离株NS5区基因片段的比较分析

1.
军事医学科学院基础医学研究所河北省卫生防疫站北京市佑安医院

摘要

对同一地区两例输血后丙型肝炎进行ＰＣＲ分型，结果为Ⅱ型。分别将其ＮＳ５区基因部分片段克隆到ｐＵＣ１８和Ｍ１３ｍｐ１８中，序列分析结果表明在ＮＳ５区基因相同区段，二者的核苷酸同源性为８９．０４％，氨基酸同源性为９０．７５％。而且在分离株Ｖ中第７０～７２位发现阅读框内终止密码子ＴＧＡ。与Ⅱ型代表株ＨＣＶＢＫ序列相比，相同区段的核苷酸和氨基酸同源性分别为：９１．５％，９１．９２％和９１．９１％，９１．９１％。对比分析表明，在同一地区基因型相同的不同分离株间ＮＳ５区基因仍存在较大变异，分离株Ｖ可能为缺陷性病毒
- 丙型肝炎病毒
- , 基因型
- , NS5区基因
- , 变异

Comparison and Analysis on HCV NS5 Gene Fragment between GenotypeⅡ isolates from Same Region

1.
Institute of Basic Medical Sciences．Academy 0f Military Medical Sciences,Beijing 100850

Abstract

Isolates from two patients of hepatitis C in the same region were classified as genotype Ⅱ by PCR method. NS5 gene fragments of HCV were cloned, and inserted into pUC19 and M13mp18, respectively.Sequence analysis showed that the homologies of the two isolates (NS5X and NS5V) in nucleotide and amino acid are 89.4% and 90.75%,respectively. Stop codon TGA was found in NS5V at 70～72 nt. The homologies of nucleotide and amino acid between the two isolates and typical genotype Ⅱ(HCV BK)are 91.5%and 91.92%,91.91% and 91.91%. The results indicated that there is relatively high variation in HCV NS5 region between isolates of same genotype from same area, and suggested that the isolate of NS5V may be a defective virus.
- Hepatitis C virus Genotype NS5 gene Variation

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Comparison and Analysis on HCV NS5 Gene Fragment between GenotypeⅡ isolates from Same Region

1. Institute of Basic Medical Sciences．Academy 0f Military Medical Sciences,Beijing 100850

Abstract: Isolates from two patients of hepatitis C in the same region were classified as genotype Ⅱ by PCR method. NS5 gene fragments of HCV were cloned, and inserted into pUC19 and M13mp18, respectively.Sequence analysis showed that the homologies of the two isolates (NS5X and NS5V) in nucleotide and amino acid are 89.4% and 90.75%,respectively. Stop codon TGA was found in NS5V at 70～72 nt. The homologies of nucleotide and amino acid between the two isolates and typical genotype Ⅱ(HCV BK)are 91.5%and 91.92%,91.91% and 91.91%. The results indicated that there is relatively high variation in HCV NS5 region between isolates of same genotype from same area, and suggested that the isolate of NS5V may be a defective virus.