Nucleotide Sequence of the Helicoverpa armigera Nuclear Polyhedrosis
Virus HindIll-K Fragment and Its Analysis

ZHANG Chuan-Xi; SUN Jian-Xin; JIANG Yo-Lei; HU Cui; WU Xiang-Fu

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April 1998

ZHANG Chuan-Xi, SUN Jian-Xin, JIANG Yo-Lei, HU Cui and WU Xiang-Fu. Nucleotide Sequence of the Helicoverpa armigera Nuclear PolyhedrosisVirus HindIll-K Fragment and Its Analysis[J]. Virologica Sinica, 1998, 13(2).

Citation: ZHANG Chuan-Xi, SUN Jian-Xin, JIANG Yo-Lei, HU Cui, WU Xiang-Fu. Nucleotide Sequence of the Helicoverpa armigera Nuclear Polyhedrosis Virus HindIll-K Fragment and Its Analysis .VIROLOGICA SINICA, 1998, 13(2) : 149.

棉铃虫核型多角体病毒Hind III-K片段的核苷酸序列及其分析

1.
浙江农业大学应用昆虫学研究所中国科学院上海生物化学研究所

摘要

测定了棉铃虫（Ｈｅｌｉｃｏｖｅｒｐａａｒｍｉｇｅｒａ）核型多角体病毒（ＨａＳＮＰＶ）基因组ＤＮＡ的ＨｉｎｄＩＩＫ片段核苷酸序列。该片段全长３２５５ｂｐ，含可编码大于４０个氨基酸残基的多肽的开放阅读框（ＯＲＦ）１５个，包括多角体蛋白（ｐｈ）基因编码区３′端４８９ｂｐ和蛋白激酶ＨａｖＰＫ基因编码区８０１ｂｐ。在ｐｈ和ＨａｖＰＫ两基因之间鉴定出一个可编码４１２个氨基酸残基的ＯＲＦ１２３６，转录方向与ｐｈ和ＨａｖＰＫ基因相反。同源分析表明，ＯＲＦ１２３６与谷实夜蛾（Ｈｅｌｉｃｏｖｅｒｐａｚｅａ）核型多角体病毒（ＨｚＳＮＰＶ）的ＯＲＦ８推导的蛋白氨基酸序列有９５．９％同源性，与苜蓿丫纹夜蛾（Ａｕｔｏｇｒａｐｈａｃａｌｉｆｏｒｎｉａ）核型多角体病毒（ＡｃＭＮＰＶ）ＯＲＦ１６２９只有２４．８％同源性，但三者均含有二组由多个脯氨酸残基串联而成的特征基序。
- 棉铃虫核型多角体病毒 3.2kb HindIII-K片段核苷酸序列 ORF 1236

Nucleotide Sequence of the Helicoverpa armigera Nuclear Polyhedrosis Virus HindIll-K Fragment and Its Analysis

1.
Institute of Applied Entomology，Zhejiang Agricultural University．Hangzhou 310029Shanghai Institute of Biochemistry．Academia Sinica．Shanghai 200031

Abstract

Nucleotide sequence of the Helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus (HaSNPV) HindIII K fragment has been sequenced. The fragment comprises 3255 bp, containing 15 ORFs that are at least 40 amino acids in length, including 489 bp of the 3′end of polyhedrin(ph) coding region and complete coding region (801bp) of the protein kinase (HavPk). An open reading frame, ORF 1236, encoding a 412 amino acid polypeptide was indentified between immediately downstream ph gene and upstream HavPK gene, but the transcriptional direction was opposite to them. Comparison of the nucleotide sequences and deduced amino acid sequences demonstrated that the ORF 1236 is closely similar to the ORF8 of H.zea single nuclear polyhedrosis virus (HzSNPV),with 95.9% amino acid identity, but is distantly related to the ORF 1629 of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV),with only 24.8% amino acid identity.
- Helicoverpa armigera Nuclear polyhedrosis virus 3255bp HindIII K fragment Nucleotide sequence ORF1236

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Nucleotide Sequence of the Helicoverpa armigera Nuclear Polyhedrosis Virus HindIll-K Fragment and Its Analysis

1. Institute of Applied Entomology，Zhejiang Agricultural University．Hangzhou 310029Shanghai Institute of Biochemistry．Academia Sinica．Shanghai 200031

Abstract: Nucleotide sequence of the Helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus (HaSNPV) HindIII K fragment has been sequenced. The fragment comprises 3255 bp, containing 15 ORFs that are at least 40 amino acids in length, including 489 bp of the 3′end of polyhedrin(ph) coding region and complete coding region (801bp) of the protein kinase (HavPk). An open reading frame, ORF 1236, encoding a 412 amino acid polypeptide was indentified between immediately downstream ph gene and upstream HavPK gene, but the transcriptional direction was opposite to them. Comparison of the nucleotide sequences and deduced amino acid sequences demonstrated that the ORF 1236 is closely similar to the ORF8 of H.zea single nuclear polyhedrosis virus (HzSNPV),with 95.9% amino acid identity, but is distantly related to the ORF 1629 of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV),with only 24.8% amino acid identity.