Construction of Helicoverpa armigera Library During Latent Periods by Nuclear Polyhedrovirus cDNA
Polym erase Chain Reaction

BAI Zhi-Jiang; ZHANG You-Qing

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August 2000

BAI Zhi-Jiang and ZHANG You-Qing. Construction of Helicoverpa armigera Library During Latent Periods by Nuclear Polyhedrovirus cDNAPolym erase Chain Reaction[J]. Virologica Sinica, 2000, 15(4): 346-349.

Citation: BAI Zhi-Jiang, ZHANG You-Qing. Construction of Helicoverpa armigera Library During Latent Periods by Nuclear Polyhedrovirus cDNA Polym erase Chain Reaction .VIROLOGICA SINICA, 2000, 15(4) : 346-349.

PCR介导HaNPV罹病幼虫潜伏期cDNA文库的构建

白志强 ,
张友清

1.
中国科学院武汉病毒研究所，武汉430071

摘要

以罹病棉铃虫幼虫为材料提取总RNA ,反转录合成cDNA第一链 ,加oligo(dG)同聚尾 ,PCR扩增合成双链cDNA ,克隆到 pGEM T质粒载体中。随机筛选文库中阳性克隆 ,经酶切分析 ,cDNA插入片段大小在 0 .3～ 1.1kb之间。文库中原代重组子数为 1.66 10 5,重组百分比为 87.8%。重组质粒的杂交分析表明 ,文库中HaNPV基因的cDNA克隆所占比例超过 50 %。
- 中国棉铃虫核型多角体病毒
- , 聚合酶链反应
- , 克隆
- , cDNA文库

Construction of Helicoverpa armigera Library During Latent Periods by Nuclear Polyhedrovirus cDNA Polym erase Chain Reaction

1.
Wuhan

Abstract

A general cDNA library which was from infected larvae dueing the latent periods has been constructed. The first cDNA strand was synthesized from total RNA by M MLV transcriptase. After oligo(dG) tailing the cDNA was amplified by polymerase chain reaction. The double strands cDNA were ligated to pGEM T easy vectors. Screeing of cDNA library showed that the length of inserts was from 0.3 to 1.1 kilobases. The capacity of cDNA library was 1.6610 5 clones. Moreover, the number of cDNA clones of HaNPV was more than 50%.
- Helicoverpa armigera nuclear polyhedravirus
- , Polymerase chain reaction
- , Clone cDNA library

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction of Helicoverpa armigera Library During Latent Periods by Nuclear Polyhedrovirus cDNA Polym erase Chain Reaction

1. Wuhan

Abstract: A general cDNA library which was from infected larvae dueing the latent periods has been constructed. The first cDNA strand was synthesized from total RNA by M MLV transcriptase. After oligo(dG) tailing the cDNA was amplified by polymerase chain reaction. The double strands cDNA were ligated to pGEM T easy vectors. Screeing of cDNA library showed that the length of inserts was from 0.3 to 1.1 kilobases. The capacity of cDNA library was 1.6610 5 clones. Moreover, the number of cDNA clones of HaNPV was more than 50%.