Study on the Feline Caticivirus Infected Tiger

FAN Quan-Shui; JIA Xian-Zhu; QIU Wei; HUANG Geng; HE Hong-Ban; ZHANG Jian-Bin; JIAO Jun; TU Chun; JU Chi-Huan; LI Jin-Zhong; WU Yin-Lian; YAN Shen-

PDF
Cite
Share

facebook

twitter

google

linkedin

August 2000

FAN Quan-Shui, JIA Xian-Zhu, QIU Wei, HUANG Geng, HE Hong-Ban, ZHANG Jian-Bin, JIAO Jun, TU Chun, JU Chi-Huan, LI Jin-Zhong, WU Yin-Lian and YAN Shen-. Study on the Feline Caticivirus Infected Tiger[J]. Virologica Sinica, 2000, 15(4): 373-378.

Citation: FAN Quan-Shui, JIA Xian-Zhu, QIU Wei, HUANG Geng, HE Hong-Ban, ZHANG Jian-Bin, JIAO Jun, TU Chun, JU Chi-Huan, LI Jin-Zhong, WU Yin-Lian, YAN Shen-. Study on the Feline Caticivirus Infected Tiger .VIROLOGICA SINICA, 2000, 15(4) : 373-378.

老虎感染猫传染性鼻-结膜炎病毒的研究

1.
中国人民解放军农牧大学军事兽医研究所，长春130062

摘要

用猫肾传代细胞从发病虎的病料中分到了一株杯状病毒粒子样病毒。该病毒大小为 35～ 39nm、无囊膜、在胞浆中病毒前体呈晶格状排列 ,抗乙醚、不能被 5 IUDR所抑制 ,能被来源于标准疫苗的猫传染性鼻结膜炎病毒 (或杯状病毒Felinecalicivirus ,FCV)抗血清所中和 ,人工感染猫出现典型的FCV感染症状。RT PCR能扩增出与设计值相符的电泳带 ,PCR产物直接测序结果与Genebank发表的FCV序列比较 ,具有较高的同源性。系统鉴定证明所分离的这株病毒为FCV强毒株 ,从老虎中分到FCV在世界上尚属首次报道。
- 虎
- , 猫
- , 猫杯状病毒

Study on the Feline Caticivirus Infected Tiger

1.
The VeterinaryI nstitute，Uniwersity of Agriculture and Animal Science，Changchun 130062，China

Abstract

A virus strain like calicivirus was isolated from organs of tiger with feline kidney (FK) cell. The viruses were nonenveloped, 35～39 nm in diameter, multiplied in cytoplasm and put in crystal order. The viruses multipied easily in feline kidney cell cultures and produced cytopathic effects (CPE). Virus cross neutralization (VN) showed that the virus is closely relation with standard vaccine virus strain. Felines infected with FK cell cultures viruses clinically appeared FCV signs. The RT PCR amplified product around 540 bp which was the same as result of Designed. The PCR products were directly sequenced. The gene sequences were compared with FCV strains extracted from the GenBank database and showed 87% identity. Systematical identification has proved that the viruse isolated is feline calicivirus of tiger. This is the first report of feline calicivirus infected tiger in China.
- Tiger Feline
- , Feline
- , caliciviruX

References
Proportional views

PDF

Article Metrics

Article views(3801) PDF downloads(769) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on the Feline Caticivirus Infected Tiger

1. The VeterinaryI nstitute，Uniwersity of Agriculture and Animal Science，Changchun 130062，China

Abstract: A virus strain like calicivirus was isolated from organs of tiger with feline kidney (FK) cell. The viruses were nonenveloped, 35～39 nm in diameter, multiplied in cytoplasm and put in crystal order. The viruses multipied easily in feline kidney cell cultures and produced cytopathic effects (CPE). Virus cross neutralization (VN) showed that the virus is closely relation with standard vaccine virus strain. Felines infected with FK cell cultures viruses clinically appeared FCV signs. The RT PCR amplified product around 540 bp which was the same as result of Designed. The PCR products were directly sequenced. The gene sequences were compared with FCV strains extracted from the GenBank database and showed 87% identity. Systematical identification has proved that the viruse isolated is feline calicivirus of tiger. This is the first report of feline calicivirus infected tiger in China.