Study on the Phage Display Expressing Anti-H antavirus Capsid Protein scFv

LI Guang-Fu; TANG Jia-Qi; CHEN Yu-Ping; WANG Yan; CAO Min; LI Xian-Fu; BO Xiu-Zhen

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August 2000

LI Guang-Fu, TANG Jia-Qi, CHEN Yu-Ping, WANG Yan, CAO Min, LI Xian-Fu and BO Xiu-Zhen. Study on the Phage Display Expressing Anti-H antavirus Capsid Protein scFv[J]. Virologica Sinica, 2000, 15(4): 409-413.

Citation: LI Guang-Fu, TANG Jia-Qi, CHEN Yu-Ping, WANG Yan, CAO Min, LI Xian-Fu, BO Xiu-Zhen. Study on the Phage Display Expressing Anti-H antavirus Capsid Protein scFv .VIROLOGICA SINICA, 2000, 15(4) : 409-413.

噬菌体表面表达抗汉坦病毒衣壳蛋白单链抗体的研究

1.
南京军区军事医学研究所,南京210002

摘要

噬菌体展示表达 (Ｐｈａｇｅｄｉｓｐｌａｙｅｘｐｒｅｓｓｉｏｎ)的主要特点是将特定分子的基因型和表型统一在同一噬菌体颗粒内 ,其基因组中含有表达蛋白基因 ,在噬菌体表面进行特定的表达。该技术为研制工程抗体提供一新的途径 ,在九十年代逐渐被人们认识并得到极其广?..
- 单链抗体
- , 肾综合征出血热病毒
- , 噬菌体表面表达

Study on the Phage Display Expressing Anti-H antavirus Capsid Protein scFv

1.
Institute of

Abstract

The expression vector of anti Hantavirus(HV) capsid protein scFv was constructed by DNA recombination and PCR technique,and expressed in E.coli . The expressed scFv was fused with the phage GeneⅢ protein, and located on the surface of phage.The sequencing of scFv demonstrated that the sequence of scFv is consistent with that of cloned antibody variable region from F3 strain hybridom against HV capsid protein.The expressed scFv was proved to be able to bind HV antigen by ELISA.
- scFv
- , Hantavirus
- , Phage display expressio

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on the Phage Display Expressing Anti-H antavirus Capsid Protein scFv

1. Institute of

Abstract: The expression vector of anti Hantavirus(HV) capsid protein scFv was constructed by DNA recombination and PCR technique,and expressed in E.coli . The expressed scFv was fused with the phage GeneⅢ protein, and located on the surface of phage.The sequencing of scFv demonstrated that the sequence of scFv is consistent with that of cloned antibody variable region from F3 strain hybridom against HV capsid protein.The expressed scFv was proved to be able to bind HV antigen by ELISA.