生后2～3 的昆明乳鼠．每只腹腔接种0 05 mL 100十半数致死量的陈株汉坦病毒，于接种后不同时间点 处死动物，取其帖组织常规固定，石蜡包埋制备5岬连续组织切片，用免疫组化法拉测组织中的病毒抗原及热体 兜蛋白70的表造，用j 聚焦显微镜观察二者之间的关系。结果发现，感染了病毒的组织能够稳定地拉测到热休克 蛋白70的表达．而病毒 I 的组织则检测不到热休克蛋白70的表=选．且二者有共定位关系，其分布与组织病变 一 致 这与在病毒感染的VeroE6细胞及EHF病人尸检组织中得到的结果一致．说明病毒感染可诱导热休克蛋白70 的表达．后者可能具有保舟’’ill织避免或减轻损伤的作用

Sueking mice．2～3 day after birth，were intraperitoneally inoculated with 0 O5mL F1M lIl 0f Chen Strain Hantaan virus At different time point after inoculation，the brains were taken，rou— tinely fixed and embedded in paraffin for preparing 5 um serial sections Traditional and confocal im— munochemicaI detection of viral antigens and heat shock protein 70 were performed to explore the cere— hral stress response after viral infection．The results showed that HSP70 imm ur~reactivities could be stably detected in the viral antigen positive neurons，hut not in the viral antigen negative or uninfected control tissues By confocaI microscopic examination．the H趼，70 and viral antigens were colocolized in the neuronal cytoplasm ．Our result，comparable to our previous findings in human tissues and culture cells。indicated that Hantavirus infection can induce the expression of H趼，70 in the infected cells。and HSP70 expresion might he necessary hut not sufficient to keep the cell surviva1．