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Volume 16 Issue 4
August 2001

    WU Dong, HU Zhihong, Just M and CHEN Xinwen. Sequence Analysis of Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus odv-e66 Gene[J]. Virologica Sinica, 2001, 16(4): 330-337.
    Citation: WU Dong, HU Zhihong, Just M, CHEN Xinwen. Sequence Analysis of Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus odv-e66 Gene .VIROLOGICA SINICA, 2001, 16(4) : 330-337.
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    棉铃虫单核衣壳核多角体病毒囊膜蛋白odv．e66基因 及其邻近区域的序列分析

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      1中国科学院武汉病研究所无脊推动物病毒学联台开放宴验室，湖北武汉430071~ 2．1aboratory 0f Virology，Wagemngen University，Bennenhaven II，6709PD Wageningen，the Nethedands

    • 杆状病毒ODV．E66蛋白是包涵体来源病毒(ceclusion-de~ved viIll8，0DV)囊膜的结构蛋白，ODV囊膜对0DV的 稳定性和感染性具有重要作用。本文报道了H且sNPv。 66基因及其邻近区域共4 2376v的核苷酸序列及其分析 结果。HaSNPV的odv-e66基因编码区全长2 019bp，推测编码一个由672个氨基酸残基组成的，分子量为74．51~D的蛋白质。在起始密码子m 上游具有杆状病毒晚期转录起始信号ATAAG。与其他杆状病毒ODV-E66的氪基酸序列比较分显示HaSNPVODV-E66蛋白具有多个保守区域，包括N末端的强疏水功能区、序列中部的一个可能的棱定位信号RⅪ ，两个1．eu—zipper以及5个跨膜区。~ -e66基因上游的两个0RF分别与AddNPV的o,Do~和0r九09具有同源性．下游的ORF与LsNPV的p13基因具有同源性。 ODV E66 is the major protein of occlusion derived virions envelope of baculovirus.An odv e66 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified and sequenced.The HaSNPV odv e66 gene had a size of 2 109 bp and potentially coding a protein of 672 amino acids,with a molecular weight of 74.5 kD.A late transcriptional motif,ATAAG,was found at 20～ 24nt uppstream of the translational start codon ATG.There was a TATA box,a GATA box and a CAGT motif at 13nt, 92nt, 141nt upstream of ATG,respectively.Amino acid sequences alignment with so far identified baculovirus’ODV E66 had revealed a conserved hydrophobic region at the N terminal of ODV E66 proteins.A potential nuclear locating signal RR(K)IW was found conserved in all ODV E66 proteins.Two leu zipper motifs were found in the middle of ODV E66 proteins.Six potential transmembrane regions were found in HaSNPV ODV E66 protein.Two of them were conserved in the other baculoviruses’.Analysis with computer shown

    Sequence Analysis of Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus odv-e66 Gene

    • 1.

      1中国科学院武汉病研究所无脊推动物病毒学联台开放宴验室，湖北武汉430071~ 2．1aboratory 0f Virology，Wagemngen University，Bennenhaven II，6709PD Wageningen，the Nethedands

    • ODV E66 is the major protein of occlusion derived virions envelope of baculovirus.An odv e66 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified and sequenced.The HaSNPV odv e66 gene had a size of 2 109 bp and potentially coding a protein of 672 amino acids,with a molecular weight of 74.5 kD.A late transcriptional motif,ATAAG,was found at 20～ 24nt uppstream of the translational start codon ATG.There was a TATA box,a GATA box and a CAGT motif at 13nt, 92nt, 141nt upstream of ATG,respectively.Amino acid sequences alignment with so far identified baculovirus’ODV E66 had revealed a conserved hydrophobic region at the N terminal of ODV E66 proteins.A potential nuclear locating signal RR(K)IW was found conserved in all ODV E66 proteins.Two leu zipper motifs were found in the middle of ODV E66 proteins.Six potential transmembrane regions were found in HaSNPV ODV E66 protein.Two of them were conserved in the other baculoviruses’.Analysis with computer shown
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      Sequence Analysis of Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus odv-e66 Gene

      • 1. 1中国科学院武汉病研究所无脊推动物病毒学联台开放宴验室，湖北武汉430071~ 2．1aboratory 0f Virology，Wagemngen University，Bennenhaven II，6709PD Wageningen，the Nethedands

      Abstract: ODV E66 is the major protein of occlusion derived virions envelope of baculovirus.An odv e66 gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus was identified and sequenced.The HaSNPV odv e66 gene had a size of 2 109 bp and potentially coding a protein of 672 amino acids,with a molecular weight of 74.5 kD.A late transcriptional motif,ATAAG,was found at 20～ 24nt uppstream of the translational start codon ATG.There was a TATA box,a GATA box and a CAGT motif at 13nt, 92nt, 141nt upstream of ATG,respectively.Amino acid sequences alignment with so far identified baculovirus’ODV E66 had revealed a conserved hydrophobic region at the N terminal of ODV E66 proteins.A potential nuclear locating signal RR(K)IW was found conserved in all ODV E66 proteins.Two leu zipper motifs were found in the middle of ODV E66 proteins.Six potential transmembrane regions were found in HaSNPV ODV E66 protein.Two of them were conserved in the other baculoviruses’.Analysis with computer shown

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